小鼠死后脑RNA降解与死亡时间的相关性

目的 探讨小鼠死后不同时间和温度下脑组织β-actin mRNA、18S rRNA的降解规律与死亡时间的关系.方法 将24只健康成年C57BL/6小鼠随机分为2组,每组12只,断颈法处死后分别置于不同的环境温度中(4℃、37℃),恒定环境湿度为80%.分别于死后即刻和0.5、2、6、24、48h取小鼠脑组织进行总RNA...

Relationship between RNA degradation and postmortem interval in mice

Objective To investigate the degradation changes of β-actin mRNA and 18S rRNA in different time points and temperature after death,and to explore the relationship between the changes and postmortem interval（PMI） in the brain of mice.Methods Twenty-four health adult C57BL/6 mice were randomly divided into two groups（12 each group）.They were sacrificed by cervical dislocation and placed in chamber with two different temperature（4 ℃ and 37 ℃,humidity was 80%）.The mice brains were sampled at 6 different time points（immediately,0.5 h,2 h,6 h,24 h,48 h）,and total brain RNA were extracted.Ct value of each sample was obtained using RT-PCR and real-time PCR technology,and β-actin mRNA and 18S rRNA content ratio was calculated.The correlation between the content ratio and PMI was expressed using statistical regression analysis.Results At 37 ℃,RNA degradation rate was faster than 4 ℃,which showed that there was correlation between temperature and RNA degradation.Comparing with the stability of β-actin mRNA,18S rRNA was more stable.Conclusion The study on degradation of β-actin mRNA and 18S rRNA in mice brain using real time PCR technology could provide a new theoretical basis for estimation of PMI and would be supplementary to the traditional methods.