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DNA-based identification of big cats and traditional Chinese medicine artifacts in the Czech Republic
Affiliation:1. Forensic DNA Service, Budinova 2, 180 81 Prague 8, Czech Republic;2. Bulovka University Hospital, Prague, Czech Republic;3. Charles Univ Prague, Institute for Environmental Sciences, Prague, Czech Republic;4. Department of Forensic Medicine, Second Faculty of Medicine, Charles University, Czech Republic
Abstract:The aim of this study is to provide an overview of ongoing research on and the development of identification tools for big cats (Panthera tigris, Panthera leo, Panthera pardus, …). The set of tools includes a species-specific RTsingle bondPCR quantitation system (nuclear and mitochondrial), STR multiplexes, a rapid system for big cat species determination, and a database solution.
Keywords:Animal DNA typing  DNA quantitation  STR multimix  Barcoding  Pantheridae  Traditional Chinese medicine
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