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中国汉族人群 8个 STR位点荧光标记同步检测及其频率分布
作者姓名:Jiang XH  Li J  Yu J  Huang B
作者单位:辽宁省刑事科学技术研究所,
摘    要:目的 对血液等微量生物学检材进行 8个 STR多态性位点及一个性别鉴定位点的复合检测 ,并调查了 350名中国汉族无关个体上述基因位点等位基因分布情况。方法 所选位点为 vWA、 TH01、 TPOX、 CSF1PO、 D5S818、 D13S317、 D7S820、 D16S539及性别鉴定位点 Amelogenin,应用荧光染料标记引物,利用 PE- 377 DNA片段分析仪对扩增产物进行基因分型。结果 共检出 63个等位基因及两个性别决定基因 ,总鉴别机率( TDP)值达 99.999 999 98%,对法医学常见极微量生物学检材的检测获得成功, DNA模板需要量为 0.5~ 1.0ng,通过家系调查,证明上述位点遗传稳定,符合孟德尔遗传规律。结论 上述 8个 STR多态性位点具备了个体认定能力 ,是对微量生物学检材进行个体识别鉴定的理想方法。

关 键 词:STR位点复合检测  荧光标记  微量生物学检材
文章编号:1004-5619(2001)02-0089-04
修稿时间:2000年5月22日

Chinese Hans population study of fluorescent labelled 8 STR multiplex system
Jiang XH,Li J,Yu J,Huang B.Chinese Hans population study of fluorescent labelled 8 STR multiplex system[J].Journal of Forensic Medicine,2001,17(2):89-92.
Authors:Jiang X H  Li J  Yu J  Huang B
Institution:Liaoning Criminal Science & Technology Institue, Shenyang 110032.
Abstract:OBJECTIVE: Chinese Hans population study of 8 STR loci was carried out in 350 unrelated individuals. METHODS: The 8 STR loci(vWA, TH01, TPOX, CSF1PO, D5S818, D13S317, D7S820, D16S539) and a sexual distinction locus Amelogenin, were amplified simultaneously in one PCR reaction tube, the products were separated by electrophoresis with PE-377 DNA sequencer, and analyzed by Genescan analysis software, then the accurate PCR fragment length and designation of genotypes were finished automatically by the sequencer. RESULTS: 63 alleles and 2 sex determination alleles were found in Hans population altogether; the minimum allele frequency in Chinese Hans population at these loci was 0.17%, the maximum was 50.17%; the fragments were 119-327 base pairs long; the TDP of this system reached 99.99999998%; this system inherited steadily, and was accord with Mandel inheritance rule, no gene mutation was found, all loci met Hardy-Weinberg equilibrium, but the frequencies of Hans population differed from those of African-Americans, Caucasion-Americans and Hispanic-Americans. CONCLUSION: This method is good for identification of little amount and degraded DNA samples from blood stain, semen stain mixed with vaginal excretion, hair root, cigarette root, muscle tissue and ancient bone. It can also make a foundation of establishing the National DNA Database.
Keywords:STR multiplex  population study  forensic application
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