Application of plant DNA markers in forensic botany: genetic comparison of Quercus evidence leaves to crime scene trees using microsatellites

As highly polymorphic DNA markers become increasingly available for a wide range of plant and animal species, there will be increasing opportunities for applications to forensic investigations. To date, however, relatively few studies have reported using DNA profiles of non-human species to place suspects at or near crime scenes. Here we describe an investigation of a double homicide of a female and her near-term fetus. Leaf material taken from a suspect's vehicle was identified to be that of sand live oak, Quercus geminata, the same tree species that occurred near a shallow grave where the victims were found. Quercus-specific DNA microsatellites were used to genotype both dried and fresh material from trees located near the burial site and from the material taken from the suspect's car. Samples from the local population of Q. geminata were also collected and genotyped in order to demonstrate that genetic variation at four microsatellite loci was sufficient to assign leaves to an individual tree with high statistical certainty. The cumulative average probability of identity for these four loci was 2.06x10(-6). DNA was successfully obtained from the dried leaf material although PCR amplification was more difficult than amplification of DNA from fresh leaves. The DNA profiles of the dried leaves from the suspect's car did not match those of the trees near the crime scene. Although this investigation did not provide evidence that could be used against the suspect, it does demonstrate the potential for plant microsatellite markers providing physical evidence that links plant materials to live plants at or near crime scenes.     

Pollen on grass clippings: putting the suspect at the scene of the crime.

In a case of alleged sexual assault, the pollen content of samples of grass clippings and soil from the suspect's clothing and shoes was compared to that of a sample of grass clippings from the alleged crime scene (a grassy area) to determine whether or not the suspect had been at the scene. The clothing and shoe samples showed a very strong correlation with each other and with the sample from the alleged crime scene in the combination of the different types of pollen present, very strongly supporting the contention that the suspect had been at the scene.     

系列杀人案件的成因、特点及侦查对策

发生系列杀人案件的原因取决于作案人的犯罪心理,谋财害命、报复杀人、滥杀无辜和杀人灭口是导致系列杀人案件的主要成因。系列杀人案件的特点体现在作案人的行为特征、作案人的个性特征和犯罪现场的痕迹物证三个方面。在侦查过程中,应引进利用“软件”串并案件的理念以拓展串并案途径,过细勘查犯罪现场,建立失踪人口管理机制以及时发现和串并系列杀人隐案,严格遵循公安部“侦破系列杀人案件工作机制”的要求规范侦查工作,引入和加强犯罪心理画像、犯罪现场重建和辩证侦查思维等现代侦查和取证方法。     

植物DNA检验技术在命案现场重建中的应用策略

植物DNA检验技术是利用植物遗传性状的稳态性对关联植物物证进行检验鉴定的手段。将该技术应用于现场重建,应基于植物物证与犯罪嫌疑人、被害人及其活动环境具有＂重大关联性＂。从命案现场重建的需求上看,应围绕犯罪嫌疑人及其可控物品中附着植物与现场植物的种属同一性判断、被害人尸体（尸块或尸骸）及其随附物品中附着植物与中心现场植物的种属同一性判断、疑似侵害物及其附着植物与嫌疑人行为关联植物的种属同一性判断等三个角度或层次进行检验和综合分析。植物DNA检验技术可阐明物证的时空运行停顿规律,为命案现场重建工作提供一种参考性解决方案。     

Piquerism in homicide: A knife wound analysis of a sexually motivated serial killer

As part of a wider research project into paraphilic disorders and homicide, a case is presented of a piquerist who achieved sexual satisfaction through the infliction of stabbing wounds. Against a background of sexual domination with willing and unwilling sexual partners, the offender stabbed four men to death. A study of the resulting autopsy reports, in association with the offender's testimony and crime scene documentation, was undertaken to discern a piquerist crime scene signature. An excessive number of stabbing wounds, the targeting of sexual areas, and a variation in wound size and depth were found to be evidence of piquerism. Although there was opportunity to commit a sexual assault upon victims, the preference was to penetrate them with a knife. It is contended that, even without any evidence of sexual assault at a crime scene, a knife wound analysis can reveal a sexual motivation in some cases of homicide.     

Use of fecal material to associate a suspect with a crime scene: report of two cases

Fecal matter analyses were applied to two cases: a homicide and a robbery. Scrapings of fecal matter removed from samples of clothing obtained from a homicide suspect were examined for their plant cell and cell fragment content and compared with fecal matter from a rape-homicide victim and scrapings from her clothing. Scrapings were hydrated and examined microscopically. Types of food plants were identified from the observed cells by comparison with known food plants. A similar analysis was conducted on the clothing of a robbery suspect and compared with fecal material left at the crime scene. The results showed that, respectively in the two cases, the reference samples were remarkably similar, if not identical, to those from the suspects' clothing.     

试述杀人案中熟人关系的刻画

现场分析在命案侦破过程中起着重要作用,案犯刻画是现场分析内容之一,现场分析时如能刻画为熟人作案,无疑对确定侦查方向具有重要价值。以往有学者从不同角度论述过如何判断犯罪加害人与被害人之间的关系,而随着社会的不断发展,人们获取各类知识和能力的途径也在不断增加,犯罪形式和作案手段也呈多样化改变,有些案件加害人的反侦查意识也在不断提高,传统的经验和判断指标在现有的案件中需要加以补充和修正。因此,有必要对如何判断熟人作案进行重新梳理,以适应现场分析的需要。     

Crime Scene Staging in Homicide

A nonrandom national U.S. sample of 946 homicide crime scenes--supplied by the FBI Behavioral Science Unit for purposes of research--was studied to delineate the prevalence, types, levels, and motives for staging in domestic, nonserial sexual, serial sexual, and general felony homicides. Stagers were found to be a relatively small group who employ a variety of methods to alter the crime scene in an attempt to redirect the investigation away from themselves as logical suspects. Results also suggest that different types of homicides have different staging rates based primarily on the relationship (or connection) between offender and victim. Implications for investigations and understanding this type of crime scene behavior are discussed.     

疑罪问题研究

疑罪是指在刑事诉讼活动中,因证据不足而对犯罪嫌疑人、被告人是否构成犯罪以及罪行轻重、此罪彼罪与一罪数罪等方面难以作出正确判断的情况。疑罪的基本特征是证据不足。疑罪可分为罪与非罪之疑罪、情节轻重之疑罪、此罪彼罪之疑罪和一罪数罪之疑罪。疑罪产生的原因是多方面的。认定疑罪要注意与疑难刑事案件相区别,把握好疑罪的认定标准,掌握好疑罪的处理原则:罪与非罪之疑适用疑罪从无;情节轻重之疑、此罪彼罪之疑和一罪数罪之疑适用疑罪从宽。贯彻好疑罪的处理原则有着重要的理论和实践意义。文章还就刑事诉讼各阶段如何具体贯彻疑罪的处理原则提出了建设性的意见。     

Suicide by driving an awl into the brain

In this report, we describe an unusual method of suicide. A 35-year-old man with a history of manic-depressive psychosis and alcohol abuse killed himself by driving an awl into his brain. A theory of the reason for using this bizarre method is discussed, and the well-known question of homicide versus suicide in crime scene investigation is illustrated.     

A case of suicidal hanging staged as homicide

Suicides staged as homicides are rarely encountered by crime scene investigators. The case of one such staged homicide is presented in which the victim used restraints during a hanging. No other cases of suicidal hangings staged as homicides could be found in the forensic literature. Similar cases should be reported so additional data can be gained from these deaths to help reveal indicators of suicide rather than homicide.     

Immunohistochemical analysis of forensic evidence from a double homicide

We report the use of immunohistochemical staining for analysis of forensic evidence from a double homicide. A 38-year-old woman and her 7-year-old daughter were murdered by multiple blows to the head and face with a tomahawk, resulting in multiple fragments of brain tissue scattered about the murder scene. The victims' husband and father was the main suspect, who maintained that he was out of town on business during the evening of the murders. However, a shirt taken from the suspect's car on the morning after the murders (secured by the police before the suspect visited the murder scene) was found to have two small stains. DNA analysis on the stains showed the presence of the deceased wife's DNA, and immunohistochemical stains on shirt fragments conclusively documented the presence of deep central nervous system tissue, providing the critical piece of evidence needed to arrest and prosecute the suspect. This report demonstrates that shirt or similar cloth fragments can be processed into paraffin blocks and subsequently immunostained to search for and classify types of tissue fragments that may be present on the fabric.     

采用纯CO气体杀人案件的分析

一氧化碳(CO)气体中毒致人死亡多见于自杀和意外事件,采用纯CO气体杀人的案例国内罕有报道。本文报道了一起采用瓶装纯CO气体致人中毒死亡的案例,作案方式隐蔽,作案手段鲜见,且伪造了作案现场,给侦查带来一定困难。经刑事技术人员认真细致地现场勘查、尸体检验和物证提取检验,结合调查访问,最终明确了案件性质,认定了犯罪嫌疑人,全面还原了其作案过程。笔者总结分析了该案刑事技术工作中的要点和难点,希望能对同行们有所帮助。     

故意杀人罪的手段残忍及其死刑裁量——以刑事指导案例为对象的研究

故意杀人罪在死刑适用案件中占有较大比重，其死刑裁量对于减少和控制死刑具有重要意义。而手段残忍是司法实践中故意杀人罪死刑裁量的重要因素，并且独立于故意杀人罪的情节严重、情节恶劣以及后果严重等评价性用语。通过对十个被司法机关认定为故意杀人手段残忍的典型案例的探讨，可以认为故意杀人罪的手段残忍是指，在杀人过程中，故意折磨被害人，致使被害人死亡之前处于肉体与精神的痛苦状态。司法实践中存在着将故意杀人罪的手段残忍与情节严重、情节恶劣以及后果严重等评价性用语相混淆的现象，致使故意杀人罪的手段残忍内容宽泛，沦为一句法律套语。故意杀人罪的死刑裁量应当主要从案件性质、犯罪情节、犯罪后果、主观恶性和人身危险性等四个方面进行考量。     

An analysis of Korean homicide crime-scene actions

Recent studies have focused on how different styles of homicides will be reflected in the different types of behaviors committed by offenders at a crime scene. It is suggested that these different types of behaviors best be understood using two frameworks, expressive/instrumental aggression and planned/unplanned violence, to analyze the way the offender acts at the crime scene. Multidimensional analysis is carried out on the crime-scene actions of 70 Korean homicides. The proposed frameworks are found to be a useful way of classifying homicide offenses, assigning 80% of homicides to a dominant theme. Results also indicate that behavioral differences can be related to the differences in the offender-victim relationship. Finally, implications and suggestions for future research are discussed.     

Comparison of the elemental composition of office document paper: evidence in a homicide case

Fraudulent substitution of a page within a multiple page document such as a will or business contract, the counterfeit manufacture of paper banknotes, and linking ransom or extortion notes have all been the focus of criminal investigations at one time or another. In a recent homicide investigation, document examiners were requested to compare a threatening letter received by a business partner of the deceased with paper samples seized under warrant from a suspect's house. Through a quantitative elemental analysis of the concentrations of nine elements (Na, Mg, Al, Mn, Sr, Y, Ba, La, and Ce) within the questioned and specimen documents, determined by inductively coupled plasma-mass spectrometry (ICP-MS), it was concluded that (i) the paper of the threatening letter originated from a different source to that of the paper seized from the suspect's house and (ii) all six pages of paper seized from the suspect's house originated from the same source. This discrimination of paper is presented as both a statistical t-test analysis (99.9% confidence limit) as well as construction of an elemental fingerprint for individual replicates within the questioned and specimen sample populations. This is the first reported use of the comparison of the elemental composition of document paper, determined by ICP-MS, to assist in a criminal investigation.     

Intentional Homicide: A Fifteen-Year Study (2000–2014) at the Department of Forensic Medicine,University of Rome “Sapienza”

This study analyzes postmortem records from 260 homicide cases autopsied by the Department of Forensic Medicine in Rome from 2000 to 2014. The victims were mainly males (74%) and young (61% aged from 21 to 50 years). Although the victims were mostly Italians, the number of foreign victims (33%) has increased since 1990, primarily due to immigration. The offenders frequently used firearms (39%), particularly in multiple murders. An increase in blunt (20%) and sharp force (32%) weapons was also seen. The primary crime scene was residential (42%), and the head was the most frequently injured body region. Male victims occurred frequently in the context of organized crime (7.6%). In family or intimate-sexual relationships, women were the majority of victims (8%). Forensic pathologists play an important role during investigation. They should consider all the information available to them, including autopsy information, crime scene information, and crime investigation data.     

Impairment based legislative limits for driving under the influence of non-alcohol drugs in Norway

Bloodstains at crime scenes are among the most important types of evidence for forensic investigators. They can be used for DNA-profiling for verifying the suspect's identity or for pattern analysis in order to reconstruct the crime. However, until now, using bloodstains to determine the time elapsed since the crime was committed is still not possible. From a criminalistic point of view, an accurate estimation of when the crime was committed enables to verify witnesses' statements, limits the number of suspects and assesses alibis. Despite several attempts and exploration of many technologies during a century, no method has been materialized into forensic practice. This review gives an overview of an extensive search in scientific literature of techniques that address the quest for age determination of bloodstains. We found that most techniques are complementary to each other, in short as well as long term age determination. Techniques are compared concerning their sensitivity for short and long term ageing of bloodstains and concerning their possible applicability to be used on a crime scene. In addition, experimental challenges like substrate variation, interdonor variation and environmental influences are addressed. Comparison of these techniques contributes to our knowledge of the physics and biochemistry in an ageing bloodstain. Further improvement and incorporation of environmental factors are necessary to enable age determination of bloodstains to be acceptable in court.     

Real-time forensic DNA analysis at a crime scene using a portable microchip analyzer

An integrated lab-on-a-chip system has been developed and successfully utilized for real-time forensic short tandem repeat (STR) analysis. The microdevice comprises a 160-nL polymerase chain reaction reactor with an on-chip heater and a temperature sensor for thermal cycling, microvalves for fluidic manipulation, a co-injector for sizing standard injection, and a 7-cm-long separation channel for capillary electrophoretic analysis. A 9-plex autosomal STR typing system consisting of amelogenin and eight combined DNA index system (CODIS) core STR loci has been constructed and optimized for this real-time human identification study. Reproducible STR profiles of control DNA samples are obtained in 2 h and 30 min with ≤0.8 bp allele typing accuracy. The minimal amount of DNA required for a complete DNA profile is 100 copies. To critically evaluate the capabilities of our portable microsystem as well as its compatibility with crime scene investigation processes, real-time STR analyses were carried out at a mock crime scene prepared by the Palm Beach County Sheriff's Office (PBSO). Blood stain sample collection, DNA extraction, and STR analyses on the portable microsystem were conducted in the field, and a successful “mock” CODIS hit was generated on the suspect's sample within 6 h. This demonstration of on-site STR analysis establishes the feasibility of real-time DNA typing to identify the contributor of probative biological evidence at a crime scene and for real-time human identification.