Neonatal Limb Amputation—An Unusual Form of Postmortem Canine Predation

Cases of postmortem canine predation often involve elderly recluses and their dogs. The face, head, and genitalia are targeted. Two unusual cases of postmortem canine predation of abandoned newborns are described to demonstrate an unusual alternative pattern of mutilation related to the small size of the decedents, marked decomposition, and canine scavenging behavior. Both bodies were abandoned/concealed soon after birth and were subsequently disturbed by dogs. Both were markedly decomposed with absent arms. Other injuries included skin and soft tissue defects of the torsos, with loss of distal portions of the right foot and the left lower leg in one case. No interstitial hemorrhage was observed in any of the exposed soft tissue wounds. There were no significant head or neck injuries. These cases show that patterns of postmortem canine predation will vary depending on the age, physical characteristics, degree of decomposition, and location of decedents.     

White‐tailed Deer as a Taphonomic Agent: Photographic Evidence of White‐tailed Deer Gnawing on Human Bone

Ungulate gnawing on bone has been reported in the taphonomic and zooarchaeological literature, but there are no known reports of ungulates altering human remains. Herein, we report on the first known photographic evidence of deer gnawing human remains. As described in nonhuman scavenging literature, forking of the bone characterizes the taphonomic effect of deer gnawing in this case, which is distinct from the effect caused by other scavengers. This type of osteophagia during the winter season is consistent with previously documented behavior of deer gnawing on nonhuman bone, possibly to obtain minerals absent in their diet. In this study, we briefly discuss the distinguishing features of ungulate gnawing, the reasons for this behavior, and possible confusion with other common types of scavenging and modification. This report contributes to taphonomic literature covering the range of animal interactions with human skeletal remains.     

Multi-agent scavenging patterns in Hawai‘i: A forensic archaeological and skeletal case study

Knowledge of the behavior of local fauna can aid forensic investigators in developing awareness of site formation processes. In Hawai‘i, little has been published on the effects of feral domestic pig (Sus scrofa) and feral domestic dog (Canis familiaris) scavenging and bone dispersal on field recovery and laboratory observations. In this Pacific tropical setting, the most consequential terrestrial taphonomic agents are pigs and dogs, both in terms of hard tissue modification and dispersal of remains across the landscape. In 2017, an archaeologist discovered the remains of an unidentified decedent on the island of Kauaʻi, State of Hawai‘i during a cultural resource management survey. Subsequently, a forensic recovery team in conjunction with Kaua‘i police and crime scene investigators used archaeological techniques, including pedestrian survey, tape-and-compass, and GPS mapping, to map and recover the remains. A feral pig trail transected various areas of the recovery site and corresponded with the distribution pattern of recovered skeletal material, including both the main concentration more broadly dispersed skeletal elements. While much of the skeleton was present, missing or unrecovered skeletal elements are consistent with expectations based on existing literature. Much of the postmortem bone deformations were characteristic of marks related to feral dog and/or feral pig scavenging. These results assisted local investigators in deciding the manner of death, as well as providing the family with an accounting of the decedent’s remains for burial. Thus, forensic anthropologists and archaeologists need to understand and develop knowledge of local animal behavior to recover and interpret human remains of medicolegal significance.     

Canid scavenging/disarticulation sequence of human remains in the Pacific Northwest

Greater understanding of animal scavenging of human remains can assist forensic science investigators in locating and recovering dispersed skeletal elements, in recognizing damage produced by scavengers, and in making more informed estimates of the postmortem interval. The pattern of skeletal damage can indicate whether the body was scavenged while intact or at some time after other natural processes of disarticulation had begun. This study analyzed thirty partially to fully skeletonized human remains with respect to scavenging at the time of body discovery in order to determine if a patterned consumption sequence existed. The scavengers were primarily coyotes (Canis latrans) and domestic dogs (C. familiaris). Sixteen non-carnivore-scavenged remains were also examined and contrasted with the carnivore-scavenged sample. Observed postmortem intervals from death to recovery ranged from 4 h to 52 months. Results demonstrate that canid scavenging of human remains takes place in sequential stages: Stage 0 = no bony involvement; Stage 1 = ventral thorax damaged and one or both extremities removed; Stage 2 = lower extremity involvement; Stage 3 = only vertebral segments remain articulated; and Stage 4 = total disarticulation. Results revealed a clear correspondence between stages of disarticulation and the postmortem interval.     

Prisoners,pups, and PTSD: the grass roots response to veterans with PTSD

As the human–animal bond is increasingly recognized as therapeutic, the role of animals, most frequently canines, grows. A contemporary pairing of animals and humans can be found inside prisons. While the dogs trained by inmates are most frequently adopted out to the community, today dogs are being trained to assist veterans with post-traumatic stress disorder (PTSD) as part of a grass roots effort to fulfill the mental health needs of veterans. The growing number of veterans with PTSD and concomitant issues including addiction, unemployment, homelessness, and crime, makes the mental healthcare issues of veterans a social problem. The civilian-led effort to provide veterans with dogs is a continuation of the public’s involvement with the proliferation of prison-based animal programs. While this next generation of animal programs has overwhelming community support, it also suffers from similar limitations as its predecessors. With no universal agreement as to the training methods or level of skills needed by the dogs, their efficacy at lessening the symptoms of veterans’ PTSD remains largely unknown.     

Scavenging behavior of Lynx rufus on human remains during the winter months of Southeast Texas

Animal-scavenging alterations on human remains can be mistaken as human criminal activity. A 32-day study, documenting animal scavenging on a human cadaver, was conducted at the Southeast Texas Applied Forensic Science facility, Sam Houston State University, Huntsville, Texas. A Stealth Cam Rogue IR was positioned near the cadaver to capture scavenging activity. An atypical scavenger, the bobcat, Lynx rufus, was recorded feeding on the cadaver. Scavenging by bobcats on human remains is not a predominant behavior and has minimal documentation. Scavenging behaviors and destruction of body tissues were analyzed. Results show that the bobcat did not feed on areas of the body that it does for other large animal carcasses. Results also show the bobcat feeds similarly during peak and nonpeak hours. Understanding the destruction of human tissue and covering of the body with leaf debris may aid forensic anthropologists and pathologists in differentiating between nefarious human activity and animal scavenging.     

The Red Swamp Crayfish Procambarus Clarkii (the Louisiana Crayfish) as a Particular Scavenger on a Human Corpse

An 85-year-old man was found deceased floating in an irrigation ditch 18 days after his disappearance. During crime scene investigation, specimens of the red swamp crayfish Procambarus clarkii were found in proximity of the body. The feeding activity of these nonendemic crayfishes contributed to the formation of specific injuries on the body and in the production of a large substance defects inside the corpse. The aim of this paper was to illustrate the scavenging activity of P. clarkii on a human body and highlight the potential postmortem artefacts caused by this species. This is the first report on a real case of postmortem injuries produced by P. clarkii crayfishes on a submerged human body. So far, crustaceans are not considered useful for the estimation of the minimum postmortem interval. However, the important modifications on the corpses deriving from the activity of these animals should be kept in consideration.     

Skeletal Indicators of Shark Feeding on Human Remains: Evidence from Florida Forensic Anthropology Cases

This research examines a series of six Florida forensic anthropology cases that exhibit taphonomic evidence of marine deposition and shark‐feeding activities. In each case, we analyzed patterns of trauma/damage on the skeletal remains (e.g., sharp‐force bone gouges and punctures) and possible mechanisms by which they were inflicted during shark predation/scavenging. In some cases, shark teeth were embedded in the remains; in the absence of this evidence, we measured interdental distance from defects in the bone to estimate shark body length, as well as to draw inferences about the potential species responsible. We discuss similarities and differences among the cases and make comparisons to literature documenting diagnostic shark‐inflicted damage to human remains from nearby regions. We find that the majority of cases potentially involve bull or tiger sharks scavenging the remains of previously deceased, adult male individuals. This scavenging results in a distinctive taphonomic signature including incised gouges in cortical bone.     

The use of cadaver dogs in locating scattered, scavenged human remains: preliminary field test results

Specially trained air scent detection canines (Canis familiaris) are commonly used by law enforcement to detect narcotics, explosives or contraband, and by fire investigators to detect the presence of accelerants. Dogs are also used by police, military, and civilian groups to locate lost or missing persons, as well as victims of natural or mass disasters. A further subspecialty is "cadaver" searching, or the use of canines to locate buried or concealed human remains. Recent forensic investigations in central Alberta demonstrated that the use of cadaver dogs could be expanded to include locating partial, scattered human remains dispersed by repeated animal scavenging. Eight dog-and-handler teams participated in a two-month training program using human and animal remains in various stages of decay as scent sources. Ten blind field tests were then conducted which simulated actual search conditions. Recovery rates ranged between 57% and 100%, indicating that properly trained cadaver dogs can make significant contributions in the location and recovery of scattered human remains.     

Muti murders form South Africa: a case report

In Southern Africa, human body parts are sometimes used for medicinal (muti) purposes. The human body is seen as being very powerful. Although ritual killing for this purpose is not common, some cases have been reported. This paper reports on an unusual muti murder, where the remains of two individuals were found in a medicine man's house. Three pots were found, two of which were constructed around human skulls. Various objects, such as coins, bullets, stones, human and animal bones were found inside the pots. They were decorated with beadwork, whistles, skin bangles, etc. Osteological analysis revealed that the human remains probably belonged to a young, white adult male individual, and a juvenile individual of Negroid descent. All associated objects probably have ritual significance, and some of these are discussed.     

Animal Scavenging and Scattering and the Implications for Documenting the Deaths of Undocumented Border Crossers in the Sonoran Desert,

Since 1998, over 5500 people have died while attempting to cross the U.S.–Mexico border without authorization. These deaths have primarily occured in the Arizona desert. Despite the high volume of deaths, little experimental work has been conducted on Sonoran Desert taphonomy. In this study, pig carcasses were used as proxies for human remains and placed in different depositional contexts (i.e., direct sunlight and shade) that replicate typical sites of migrant death. Decomposition was documented through daily site visits, motion-sensitive cameras and GIS mapping, while skeletal preservation was investigated through the collection of the remains and subsequent faunal analysis. Our results suggest that vultures and domestic dogs are underappreciated members of the Sonoran scavenging guild and may disperse skeletal remains and migrant possessions over 25 m from the site of death. The impact of scavengers and the desert environment on the decomposition process has significant implications for estimating death rates and identifying human remains along the Arizona/Mexico border.     

An Investigation of Red Fox (Vulpes vulpes) and Eurasian Badger (Meles meles) Scavenging,Scattering, and Removal of Deer Remains: Forensic Implications and Applications

Within northwest Europe, especially the United Kingdom, the red fox (Vulpes vulpes) and the Eurasian Badger (Meles meles) are the largest wild scavengers capable of modifying a set of remains through scavenging. Knowledge of region-specific and species-typical scavenging behaviors of scavengers within the crime scene area and surroundings can aid in more efficient and accurate interpretations. The scavenging behaviors of captive and wild foxes and badgers were recorded and compared through actualistic methods and direct observation. The scavenging by wild foxes and badgers of surface-deposited baits and whole deer (Cervus nippon; Capreolus capreolus) in a woodland was observed and analyzed. Wild foxes were found to scavenge deer more frequently than badgers. The scavenging of deer remains by foxes was also compared with forensic cases. The scavenging pattern and recovery distances of deer and human remains scavenged by foxes were similar but were potentially affected by the condition and deposition of a body, and the presence of clothing.     

Cadaver dog and handler team capabilities in the recovery of buried human remains in the southeastern United States

The detection of human remains that have been deliberately buried to escape detection is a problem for law enforcement. Sometimes the cadaver dog and handler teams are successful, while other times law enforcement and cadaver dog teams are frustrated in their search. Five field trials tested the ability of four cadaver dog and handler teams to detect buried human remains. Human and animal remains were buried in various forested areas during the summer months near Tuscaloosa, Alabama. The remains ranged in decomposition from fresh to skeletonized. Cadaver dogs detected with varying success: buried human remains at different stages of decomposition, buried human remains at different depths, and buried decomposed human and animal remains. The results from these trials showed that some cadaver dogs were able to locate skeletonized remains buried at a significant depth. Fresh and skeletonized remains were found equally by the cadaver dogs along with some caveats. Dog handlers affected the reliability of the cadaver dog results. Observations and videotape of the cadaver dogs during field trials showed that they were reliable in finding buried human remains.     

Development of a Nomenclature System for a Canine STR Multiplex Reagent Kit*†

Abstract: Despite the popularity of dogs in US households, canine DNA evidence remains largely untapped in forensic investigations partially because of the absence of well‐defined forensic short tandem repeats (STRs), lack of standardized and validated PCR protocols, STR reagent kits, and poorly developed nomenclature. A nomenclature system was established based on internationally recognized recommendations for human forensic STRs for a recently developed canine STR reagent kit. Representative alleles were sequenced from each of the 18 STRs and the sex‐typing marker included in the kit. This study also reflects on the impact of point mutations, insertions, and deletions within and outside the STR core repeat structures. An understanding of the STRs’ sequence and repeat structures will enable development of a robust and reliable allele nomenclature and improve the accuracy and precision of allele fragment sizing in canine forensic profiling. The expected allele sizes have been calculated, and their repeat stuctures defined based on sequence information.     

Scavenger Species-typical Alteration to Bone: Using Bite Mark Dimensions to Identify Scavengers

Scavenger-induced alteration to bone occurs while scavengers access soft tissue and during the scattering and re-scavenging of skeletal remains. Using bite mark, dimensional data to assist in the more accurate identification of a scavenger can improve interpretations of trauma and enhance search and recovery methods. This study analyzed bite marks produced on both dry and fresh surface deposited remains by wild and captive red fox (Vulpes vulpes) and Eurasian badger (Meles meles), as well as domestic dog (Canis familiaris). The bite marks produced by foxes were distinguishable from those made by badgers and dogs based on ranges of mean length and breadth of pits. The dimensional data of bite marks produced by badgers and dogs were less discernible. Bone modifications vary due to a variety of factors which must be considered, such as scavenger species-typical scavenging behavior, scavenger species' dentition, condition and deposition of remains, and environmental factors.     

Spatial patterning of vulture scavenged human remains

This article presents the results of a pilot study on the effects of vulture modification to human remains. A donated body from the Willed Body Donation Program was placed at the Forensic Anthropology Research Facility (FARF), an outdoor human decomposition laboratory located at Texas State University-San Marcos. The effects of vulture scavenging on the timing and sequence, and the rate of skeletonization, disarticulation, and dispersal were observed via a motion sensing camera and direct observation. Using GIS (Geographic Information Systems) and GPS (Global Positioning System) technologies and spatial analytical methods, the transport of skeletal elements was mapped in order to analyze dispersal and terrain-influenced patterns of active vulture scavenging. Results showed that the initial scavenging took place 37 days after placement at FARF. This delay in scavenging differs from previous research. After the initial appearance of the vultures, the body was reduced from a fully-fleshed individual to a skeleton within only 5h. This underscores the potential for errors in postmortem interval estimations made at vulture scavenged scenes. Additionally, spatial analysis showed that skeletal elements were dispersed by vultures to lower elevations, and that the disarticulation and dispersal of the skeletal elements occurs early in the scavenging sequence.     

Human being eaten by his own dogs: Genetic confirmation through analysis of bones recovered in a dog's stomach content

A part of a decomposed human body from an individual was found at his home, together with the decomposed bodies of his 3 dogs. The disappearance of half of the human body was hardly explained. Hypothetically the dogs, starving, could have eaten their owner after his death. All the bodies were recovered for autopsy.Small bone fragments were recovered in one dog's stomach and identified as human by anthropological analysis. DNA was extracted and cytochrome b gene analysis was made in order to determine their origin, confirmed as human.Genetic identification allowed achieving an eight mini-STR profile with MiniFiler (Applied Biosystems) identical to the bone material collected at the victim's autopsy, confirmed that the dogs had effectively eaten their owner.The results showed that it is possible to obtain nuclear DNA in samples subjected to gastric acids and the combination of different techniques allowed us to determine, in each step, the most convenient workflow for the remains identification.     

Mitochondrial Genome DNA Analysis of the Domestic Dog: Identifying Informative SNPs Outside of the Control Region*

Abstract: While the mitochondrial control region has proven successful for human forensic evaluations by indicating ethnic origin, domestic dogs (Canis lupus familiaris) of seemingly unrelated breeds often form large groups based on identical control region sequences. In an attempt to break up these large haplotype groups, we have analyzed the remaining c. 15,484 base pairs of the canine mitochondrial genome for 79 dogs and used phylogenetic and population genetic methods to search for additional variability in the form of single nucleotide polymorphisms (SNPs). We have identified 356 SNPs and 65 haplotypes in the remainder of the mitochondrial genome excluding the control region. The exclusion capacity was found to be 0.018. The mitochondrial control region was also evaluated for the same 79 dogs. The signals from the different fragments do not conflict, but instead support one another and provide a larger fragment of DNA that can be analyzed as forensic evidence.     

Creation of training aids for human remains detection canines utilizing a non-contact, dynamic airflow volatile concentration technique

Human remains detection (HRD) canines are trained to locate human remains in a variety of locations and situations which include minimal quantities of remains that may be buried, submerged or extremely old. The aptitude of HRD canines is affected by factors such as training, familiarity with the scent source and environmental conditions. Access to appropriate training aids is a common issue among HRD canine handlers due to overly legal restrictions, difficulty in access and storage, and the potential biological hazards stemming from the use of actual human remains as training aids. For this reason, we propose a unique approach of training aid creation, utilizing non-contact, dynamic air-flow odor concentration onto sorbent materials. Following concentration, the sorbent material retains the odor from the scent source composed of volatile organic compounds. The sorbent material containing the odor can then be utilized as a canine training aid. Training materials prepared in this manner were tested under a variety of conditions with many HRD canines to demonstrate the efficacy of the new training aids. A high level of correct canine responses to the new training aids was achieved, approaching 90%, with minimal false positives.     

Copycatting the smell of death: Deciphering the role of cadaveric scent components used by detection dogs to locate human remains

Human remains detection dogs (HRDD) are commonly used by law enforcement agencies to search for cadavers. Biological material is typically used as a training stimulus, also called aids, to train dogs to recognize the smell of cadavers. While HRDD training approaches have received extensive attention, information remains limited on the olfactory cues used to train them. Here, we aimed to decipher the chemical basis of detection dog olfaction. Five specific objectives were explored to precise whether the composition or the concentration of the training aids drives the HRDDs responses. We recorded the behavioral responses of four HRDDs exposed to different cadaveric-like smells. We found that HRDDs recognized a simplified synthetic aid composed of cadaveric compounds. The lowest concentration at which HRDDs continued to perceive the cadaveric smell was determined. HRDDs were not impacted by slight modifications to the chemical composition of a blend of odors that they have been trained with. HRDDs associated sulfur and nitrogen compounds as human cadaver. Our findings highlight a lack of specificity of HRDDs to cadaveric compounds, which could lead to error of detection. Moreover, all dogs did not positively respond to the same blends, despite being trained with the same aids and procedure. However, we confirmed that dogs could be trained with a simplified blend of molecules. The chemical composition of a training aid has, therefore, high consequences on the performance of the trained animal, and this conclusion opens additional questions regarding olfaction-based detection animals.