骨骼CT三维重建图像性别判定和年龄推断研究

目的通过计算骨骼计算机断层扫描(Computed Tomography,CT)三维重建图像判定性别和推断年龄的准确率,评价利用骨骼CT三维重建图像判定性别和推断年龄的可行性。方法将法医人类学教材中性别判定和年龄推断的方法应用于539例骨骼CT三维重建图像样本,判定245例骨盆的性别,推断100例男性耻骨联合、100例男性胸骨和94例男性锁骨的年龄,计算准确率。结果骨盆性别判定准确率为98.4%,耻骨下角和坐骨大切迹是区分性别最好的两项指标。男性耻骨联合、胸骨、锁骨的年龄推断准确率在45%以上,其中锁骨双侧方程准确率最高为77%。结论利用骨骼CT三维重建图像判定性别和推断年龄具有可行性。使用大体标本建立的骨盆形态学性别判定方法可直接应用于骨骼CT三维重建图像。使用大体标本建立的男性耻骨联合、胸骨、锁骨形态学年龄推断指标、分级和评分标准以及方程需要修改和补充。     

Metric Assessment of the Pubic Bone Using Known and Novel Data Points for Sex Estimation,

Biological sex estimation of skeletal remains is essential in forensic and archaeological analyses. Anthropologists most often use the pelvis, which is the most sexually dimorphic element both morphologically and metrically. While nonmetric pubic bone features have been studied extensively, few metric studies have examined this individual bone for dimorphism. For this study, three observers examined three previously identified and ten novel measurements of the pubic body on a modern sample of isolated pubic bones from the Maricopa County Forensic Science Center (FSC), in Phoenix, Arizona (n = 400). A relationship between pubic body measurements and biological sex was demonstrated, with significant correlations. Discriminant function analyses found that five measurements, four of which were novel, discriminated between males (89%) and females (86%). Observer experience level did not significantly impact the results. These five measurements were reliable and show promise for inclusion in metric methods for assessment of sex.     

重建耻骨联合三维结构用于活体年龄推断的技术方法

目的建立基于MRI成像的快速耻骨联合三维结构重建技术方法。方法利用3.0 T超导磁共振仪对成年男性志愿者骨盆进行T1小角度快速激发3D梯度回波序列扫描,进行二值化阈值分割、区域增长提取骨盆软组织图像;运算获得耻骨联合三维结构模型雏形,经逆向工程软件降噪处理建立耻骨联合三维结构模型,并与耻骨CT扫描三维结构重建模型进行比较。结果耻骨联合面沟嵴、下端、腹侧缘(斜面)、背侧缘(斜面)、耻骨结节等结构显示完整,无明显噪点,与CT扫描三维重建模型吻合较好。结论 MRI扫描成像可实现快速有效的耻骨联合三维结构重建,可为法医活体年龄推断提供安全无辐射作用的三维可视化影像学技术方法。     

Metric assessment of race from the pelvis in South Africans

It is well known that there are measurable differences between whites and blacks in the size and proportions of skeletal components. There are also metric differences among populations within these racial phenotypes. The population specific quantification of this variation can be used to aid in the identification of racial affinity in the absence of a more racially definitive skeletal element like the skull. Therefore, the purpose of this research is to quantify these differences in the pelvis of South African whites and blacks. A sample of 400, ostensibly healthy known sex/race os coxae was examined. Skeletal material was obtained from the Pretoria and Dart collections. A series of 13 measurements were taken Data were subjected to SPSS stepwise and direct discriminant function analysis. Pubic length was chosen as best for discriminating between races for males and iliac breadth as best in females. Highest average accuracies (Function 1) were 88% for males (pubic length, greater sciatic notch posterior width, acetabulum diameter, total height) and 85% for females (pubic length, greater sciatic notch posterior width, acetabulum diameter, iliac breath). In conclusion, this research resulted in the development of standards of identification tailored to this population and unquestionably demonstrates that race differences in the skeleton are highly significant and must be considered in all skeletal analyses.     

Evaluation of the Suchey-Brooks method for aging skeletons in the Balkans

This study has been carried out to examine whether the Suchey-Brooks (S&B) methods could be successfully applied in age assessment of populations from the Balkans. The known-age sample consists of 33 females and 52 males pairs of pubic bones collected from the autopsy cases. Age estimation by S&B method showed an accuracy of 89.74% in males and 72.0% in females. Statistical analysis showed a positive correlation between the actual age of the investigated individuals and age phases obtained by the S&B method, although the mean values of the sixth age category differed significantly compared with the original model. The most reliable indicators in both sexes were the relief of the symphyseal surface, lipping, symphyseal rim, and dorsal margin. The discriminating power of these indicators was the least reliable in distinguishing S&B phases 2 and 3. Based on these results, the appropriate recommendations for aging Serbian populations are made. There was a good agreement between two observers (kappa=0.726).     

Quantitative Analysis of the Morphological Changes of the Pubic Symphyseal Face and the Auricular Surface and Implications for Age at Death Estimation

Age estimation methods are often based on the age‐related morphological changes of the auricular surface and the pubic bone. In this study, a mathematical approach to quantify these changes has been tested analyzing the curvature variation on 3D models from CT and laser scans. The sample consisted of the 24 Suchey–Brooks (SB) pubic bone casts, 19 auricular surfaces from the Buckberry and Chamberlain (BC) “recording kit” and 98 pelvic bones from the Terry Collection (Smithsonian Institution). Strong and moderate correlations between phases and curvature were found in SB casts (ρ 0.60–0.93) and BC “recording kit” (ρ 0.47–0.75), moderate and weak correlations in the Terry Collection bones (pubic bones: ρ 0.29–0.51, auricular surfaces: ρ 0.33–0.50) but associated with large individual variability and overlap of curvature values between adjacent decades. The new procedure, requiring no expert judgment from the operator, achieved similar correlations that can be found in the classic methods.     

中国汉族人耻骨性别判定的研究

目的为了探讨耻骨性别判定的更为简便可靠的方法。方法对96副已知生前确切性别的耻骨进行了性别差异的研究,其中男性耻骨73副,女性耻骨23副。测量项目有:缘枝角,耻骨宽,耻骨联合面的高及厚。结果将耻骨的测量数据输入电脑,用SPSS软件,对耻骨的测量数据进行了统计分析,建立了耻骨性别判定的判别方程。结论其性别判别率最高可达95.5%,该方法可以用于法医学实践。     

A test of the phenice method for the estimation of sex

The Phenice method for the estimation of sex of an individual from the pelvis was tested on 198 individuals of known sex from the Terry collection by an individual with training in the technique, but no additional training in forensic anthropology. Sex was correctly estimated in 88.4% of all individuals and more accurately estimated in females than in males. In a subsequent test of the same individuals by the same investigator utilizing additional nonmetric pelvic indicators, overall accuracy increased to 96.5%, with slightly greater accuracy for males. The study suggests that experience likely contributes to the accuracy of the Phenice method and that for inexperienced investigators, consultation of all pelvic indicators offers an advantage over using just the three variables in the Phenice method.     

Effects of Parturition on Pelvic Age Indicators

This research assessed whether parity has a differential effect on age indicators of the pelvis. The data were collected from the William M. Bass Donated Skeletal Collection. Age indicators of the pubic symphysis and auricular surface were assessed using the Todd, Suchey–Brooks, Lovejoy et al., and Buckberry and Chamberlain scoring systems. A transition analysis was conducted, and scoring systems of the pubic symphysis showed the parous female group transitioning between eight and 14 years earlier than the nulliparous female and male groups, and 1 and 7 years earlier using the auricular surface methods. A likelihood ratio test produced significantly different results between parous and nulliparous females using the pubic symphysis (p < 0.01) but not the auricular surface (p > 0.05). Although parous females were inaccurately assessed more often than nulliparous females, no correction of methods is necessary at this time as current standards take parity into account by utilizing a lumped female sample.     

Pubic Symphyseal Age Estimation from Three‐Dimensional Reconstructions of Pelvic CT Scans of Live Individuals

Skeletal age estimation using the pubic symphysis is a reliable and popular practice among forensic anthropologists. In instances in which accessing the skeleton is impossible, such as in the case of living individuals, three‐dimensional (3D) reconstructions of computed tomography (CT) scans can be created and analyzed for aspects of the biological profile. This study examined 3D reconstructions of pelvic CT scans of a sample of 44 live adults to estimate age using the Suchey–Brooks J Hum Evol 1990;5(3):227‐38 method. Images were created using OsiriX software (Pixmeo, Geneva, Switzerland). Estimated age ranges based on the 3D‐CT images captured the true age of the individual at the time of the CT scan 79.5% of the time with good intra‐observer reliability. The features best visualized on the 3D images included the ossific nodule and the pubic symphyseal rim. This study shows that clinical CT scanning parameters are adequate for creating 3D images to be analyzed for age using the pubic symphysis.     

Using PMCT of Individuals of Known Age to Test the Suchey–Brooks Method of Aging in Victoria,Australia

The estimation of an individual's age at death plays a critical role in the investigation of unidentified human remains. The Suchey–Brooks method, which involves the analysis of degenerative morphological changes in the pubic symphysis, is currently widely used to estimate age in adults. This study tested the applicability of the Suchey–Brooks method on a contemporary adult Australian (Victorian) subpopulation by observing three‐dimensional volume‐rendered postmortem computed tomographic (PMCT) reconstructions of the pubic symphysis of 204 individuals aged 15–100 years. Results showed the method was reliable for females (85% correct allocations) but not for males (67% correct allocations). It is therefore recommended that extreme caution be used when applying the Suchey–Brooks method for estimating age at death of an individual in this subpopulation from PMCT images. The results suggest further investigation into alternate age estimation methods is required.     

Some medicolegally important aspects of symphyseal injuries due to trauma

A total of 58 cases of fatal trauma (forensic autopsies) were investigated for lesions of the pelvic girdle, the pubic symphysis, and for injuries distant from the pelvis. Traumata of the pubic symphysis were present in 30% (n = 17) of the cases. The symphyses were examined by x-ray and macroscopically related to the main violence (Abbreviated Injury Scale [AIS]). A correlation between the latter and the severity of the resulting injuries could be deducted. Characteristic patterns of symphyseal injuries included small hemorrhages, partial and complete ruptures, and also ruptures of the ligamentary insertions, which have not yet been described. The kind and severity of these lesions related to the intensity/direction of the violence are demonstrated and discussed.     

A Test of the DSP Sexing Method on CT Images from a Modern French Sample

The hip bone is considered to be one of the most reliable indicators in sex determination. The aim of this study was to test the reliability of the DSP method for the hip bone proposed by Murail et al. (Bull Mem Soc Anthropol Paris, 17, 2005, 167) on a sample from a present‐day population in France (52 males and 54 females). Ten linear measurements were collected from three‐dimensional models derived from computed tomography images (CTI). To quantify the proportions of correct sex determinations, a more rigorous posterior probability threshold of 0.95 was applied. Using all 10 measurements, 92.3% of males and 97.2% of females were sexed correctly. The percentage of undetermined specimens varied depending on the used combination of measurements; however, all sexes were assigned with a 100% accuracy. This study proves that DSP is an appropriate and reliable tool for sex determination, based on dimensions obtained from CTI.     

Graffiti vandalism: Frequency and context differences between the sexes

This paper explores a popular yet highly destructive form of property crime — graffiti vandalism. The localities of such destruction selected for study were male and female restrooms. Three hundred and ninety-two cases were collected and analyzed. Prior research theorized the existence of a significant difference in the amount of graffiti produced between the sexes. (It was believed that males produce more graffiti than females). Analysis, however, revealed equal production of graffiti by males and females.Content differences between the sexes were hypothesized. The content categories included homosexual, heterosexual, nonsexual (humorous and political statements) and racial graffiti. The findings as they relate to content differences between the sexes are presented and discussed relative to sex-role socialization theory.     

Sex determination using nonmetric characteristics of the mandible in Koreans

The mandible is the largest and hardest facial bone and retains its shape better than other bones in the forensic and physical anthropologic field. The mandible can be used to distinguish among ethnic groups and between sexes. We examined the morphological characteristics of the mandibles of 102 Koreans of either sex. Of 13 nonmetric items of the mandible, the characteristic that best allowed the sexes to be distinguished was the contour of the lower border of the mandible: rocker-shaped mandibles predominated in males (68.1%), whereas most females (84.6%) exhibited a straight mandible. In addition, the mental region was shaped differently between the sexes: the shape of the chin in most males was generally bilobate or square (91.7%), whereas the chin in females was either square (45.5%) or pointed (54.5%). In this study, the positive predict values of male and female were 92.5% and 73.7%, respectively. Therefore, the nonmetric method used to analyze the mandible in this study can be used for sex discrimination.     

Comparison of methods to determine sex by evaluating the greater sciatic notch: Visual, angular and geometric morphometrics

Sex estimation is the first step for biological profile reconstruction of an unknown skeleton (archaeological or contemporary) and consequently for positive identification of skeletal remains recovered from forensic settings. Several tools have been developed using different osseous structures. With the intention to provide an objective method comparison, we reported the analysis of three different methods (visual, metric and geometric morphometrics) for sex assessment of the greater sciatic notch. One hundred and thirty pelvic bones (45.4% females and 54.6% males) from the National Autonomous University of Mexico Skeletal Collection pertaining to the contemporary Mexican population were analyzed. We used the ROC-analysis to test between desired false positive thresholds (1-specificity) and expected true positive rates (sensitivity) in order to predict the best approach to sex assessment. The comparison of the area under the ROC-curves shows significant differences among visual and metric methods. At the same time, the analysis suggested that higher morphological variation among the sexes is independent of the methodological approach. The results indicate that the metric (angle), with a high percent of indeterminate cases (34.6%), and visual, with 26.2% of the cases allocated as intermediate cases, were poorly accurate; we cannot recommend these techniques for sexing an unknown specimen. On the other hand, the geometric morphometrics approach improves sex estimation in 82.3% of correctly classified individuals with more than 95% of posterior probability. In addition to the method comparison, the major sexual variation of the greater sciatic notch was determined to be located on its posterior border.     

Sexual dimorphism of the hip joint in Greeks

The objective of the present study was to report our measurements of hip bones within the Greek population and review the possible implications of these differences in their health as well as in social life. For this purpose the remains of 100 male and 100 female pelvic and femoral bones were studied. The distance from the pubic tubercle to the anterior rim of the acetabulum, the acetabulum diameter between its rims, their ratio, the depth of the acetabulum, the diameter of the femoral head and the ratio between the femoral head and the diameter of the acetabulum were measured. It was found that in males, in comparison to the females, the distance from the pubic tubercle to the anterior rim of the acetabulum was smaller while the acetabulum diameter and its depth, the diameter of the femoral head and the ratio between femoral head and the acetabulum diameter were larger. The above differences reached strong statistical significance. Of the two ratios used only the first one reached statistical significance. Using this ratio alone offered the best discrimination rate of up to 99% and should be the preferred choice when available.     

Identification and analyses of female civilian victims of the 1991 war in Croatia from the Glina and Petrinja areas

Introduction

The process of recovering and identifying human remains from individual and mass graves has proven to be the most effective method of resolving the fate of missing individuals in the former Yugoslavia. These efforts have two primary objectives: medicolegal—to identify the cause of death, and humanitarian—to bring closure to living family members, thus supporting the human rights of both the living and the deceased. From 1996 to the present, the remains of 388 individuals killed during the 1991 War in Croatia were recovered from the Glina and Petrinja areas (Sisa?ko–Moslova?ka County). The purpose of this paper is to report on the demographic and taphonomic characteristics of the recovered remains, and identify factors responsible for the discrepancy in the identification ratios.

Methods

To identify factors potentially responsible for the noted discrepancy, data were collected on the taphonomic characteristics of the recovered remains, the types of burials, as well as the factors responsible for positive identification. These factors included: forensic DNA testing, dental criteria, special signs (healed fractures, etc.), clothing and personal artifacts.

Results

The age distributions between males and females in the sample are significantly different. The mean age-at-death for males is 51.6 years, for females 67.0 years. In both sexes the majority of identifications (62.9% in females and 45.9% in males) were established through a combination of biological and non-biological features. In terms of the types of graves from which the bodies were recovered, positive identification in both sexes was lowest in mass graves (61.2% for males, and 84.2% for females). Females from mass graves were, however, better preserved than males—36.6% of males recovered from mass graves were completely skeletonized, while the same was true for only 19.3% of females (χ² = 7.06, P < 0.029). Completely skeletonized remains were identified in only 65.1% of cases, while partially skeletonized and saponified bodies were identified in 80.1% of cases.

Conclusion

Since positive identification in both sexes is strongly correlated with preservation of the bodies, the reason why female identification frequencies in the Glina and Petrinja regions are higher than male frequencies lies in the fact that females recovered from mass graves were better preserved than males recovered from mass graves. Identification of the factors responsible for this is a challenge that needs to be resolved in future studies.     

Secular Change in Morphological Pelvic Traits used for Sex Estimation

This research evaluates secular change in Phenice's (Am J Phys Anthropol, 30, 1969 and 297) three morphological traits of the pubis, as described by Klales et al. (Am J Phys Anthropol, 149, 2012 and 104): medial aspect of the ischio‐pubic ramus, subpubic contour, and ventral arc. Ordinal scores were collected for these traits and compared between a sample of innominates from the historical Hamann–Todd Collection (n = 170) and modern Bass Donated Collection (n = 129). Using the Freeman–Halton test, significant differences between temporal sample score frequencies were found for all traits in females and for the subpubic contour and ventral arc in males. Despite these findings, classification accuracy using logistic regression between the temporal periods remained low (68.7%). These results suggest that secular changes in trait expression are occurring; however, sex estimation methods using these traits and created with historical samples are still applicable to modern forensic cases. In fact, the secular changes occurring in these traits contribute to better classification accuracy between sexes in modern populations.