共查询到17条相似文献,搜索用时 296 毫秒
1.
成都地区汉族Gc亚型的分布及血痕中Gc亚型的检测 总被引:2,自引:1,他引:2
作者用免疫固定薄层聚丙烯酰胺凝胶等电聚焦(PAGIF)技术,调查了成都地区无关的125名健康汉族人血清Gc亚型分布。其6种亚型频率(%)分别为:Ge1F=20.8,Ge1S=8.0,Gc1F-1S=18.4,Gc2-1F=30.4,Gc2-1S=16.0和Gc2=6.4。Gc的基因频率为:Cc~(1F)=0.452,Gc~(1S)=0.252和Gc~2=0.296。对保存于室温条件下20周的陈旧血痕进行了Gc亚型定型,获得满意结果。 相似文献
2.
Gc(维生素D结合蛋白)的遗传多态性由Hirschfeld首次发现。用免疫电泳或聚丙稀胺凝胶园盘电泳可检查Gc三种表型:Gc1-1、Gc2-1、与Gc2-2,它们受一对等位基因Gc~1与Gc~2的控制。本文调查了四川省茂汶县羌族三种Gc表型的分布,算出其基因频率,作为羌族人群亲子鉴定的理论根据。 相似文献
3.
采用超薄层聚丙烯酰胺凝胶等电聚焦法对血痕Tf亚型进行分型,并调查北京地区223名无血缘关系汉族人群Tf亚型的分布。其基因频率:TfC~10.7354,TfC~20.2377 TfDchi 0.0269。经Hardy-Weinberg吻合度检验,观察值与期望值无显著性差异(ΣX~2=0.9905 df=3 p>0.50)。Tf的个人识别率为0.4020。非父排除率为0.1813。讨论了汉族群体Tf亚型及不同地区,不同人种间Tf分布的情况。到目前为止,室温下保存8个月的血痕仍可进行Tf亚型分型。对实验中质量的控制作了探讨,采用伏时控制电泳条件。 相似文献
4.
5.
目的探讨建立Gc亚型检测的复合MS-PCR法及其应用价值。方法根据Gc基因中的2处点突变,设计2对片段相差5bp的等位基因特异性引物和1条公共引物进行复合MS-PCR,分析Gc多态性,并调查武汉地区218例汉族无关个体Gc多态性和鉴定10例亲子关系。结果复合MS-PCR检测的Gc基因型,与AmpliTypePM试剂盒的分型结果一致;武汉地区汉族人群Gc基因的3个常见等位基因Gc1F、Gc1S、Gc2的基因频率分别为0.4816、0.2592、0.2592,观察杂合度(Hobs)、期望杂合度(Hexp)、多态性信息含量(PIC)、个人识别能力(DP)、非父排除率(PE)分别为0.6193、0.6359、0.6253、0.7974、0.3480,基因型分布符合Hardy-Weinberg平衡;真三联体和非真三联体亲子鉴定各5例,前者不排除父子关系,与常规STR分型一致,后者经Gc-MS-PCR分型排除2例。结论建立复合MS-PCR法检测Gc亚型在法医物证鉴定中有实用价值。 相似文献
6.
7.
8.
本文采用薄层聚丙烯酰胺凝胶等电聚焦电泳检测红细胞及血痕酸性磷酸酶表型,并对不同条件下的血痕标本进行检测,发现室温下(15~33℃)保存的110例纱布血痕7周内可全部正确分型,21例磁板血痕9周内均可正确分型;含血量≥5λl 的血痕可被正确检出 EAP 表型;日晒、水洗、发霉等因素可影响血痕 EAP 型的正确检出。同时调查了广东人群的 EAP 表型分布,基因频率为 p~a=0. 2338,p~b=0. 7662,发现 EAP 基因频率分布存在着地区差异。 相似文献
9.
<正> 在60年代初,有人尝试用免疫电泳技术对人血痕进行 Gc 定型,均以血痕中的Gc 蛋白在电泳时,向阳极飘移而告失败。Shinomiya 和 Baelen 发现 Gc 蛋白飘移的原因是与其血痕中破碎细胞释放出来的肌动蛋白结合形成复合物所致。用4M 盐酸胍处理血痕,可使复合物解聚,恢复 Gc 的正常电泳迁移率。 相似文献
10.
11.
P Cortivo M Biasiolo L Caenazzo C Scorretti P Benciolini G Ongaro 《Zeitschrift für Rechtsmedizin》1984,93(4):311-315
The distribution of Gc phenotypes in the population of Veneto was investigated by ultrathin-layer isoelectric focusing. In our sample (n = 732) the six common phenotypes, Gc 1S, 1F, 1S1F, 2, 2-1S, 2-1F and a further phenotype, GC 1S1C3, were observed and the following frequencies calculated: Gc 1S = 0.560792; GC 1F = 0.159153; Gc2 = 0.277323; Gc 1C3 = 0.002732. Our gene frequencies have been compared with those found in other populations. 相似文献
12.
13.
The limits of determination of Gc subtypes in bloodstains were compared between the immunofixation method and the sulfosalicylic acid precipitation method using isoelectric focusing on polyacrylamide gel. By the immunofixation method Gc subtyping in bloodstains was successfully made at 37 degrees C after 7 weeks, at room temperature after 17 weeks and at 4 degrees C even after 25 weeks storage. By the sulfosalicylic method Gc subtypes were no longer able to be determined a few weeks after stain formation. The superiority of the results obtained by the immunofixation method makes it the recommended method for the Gc subtyping from bloodstains in medicolegal practice. 相似文献
14.
A Alonso 《Journal of forensic sciences》1988,33(5):1267-1272
The identification of group specific component (Gc) subtypes derived from blood-stains by separator isoelectric focusing in micro-ultrathin polyacrylamide gels (interelectrode distance: 50 mm) containing 4.5 to 5.4 pharmalytes is described. The separation achieved between Gc 1F and Gc 1S bands is compared favorably with that obtained using separator isoelectric focusing in conventional polyacrylamide gels dimensions (interelectrode distance: 110 to 120 mm). The technique is rapid and economical, and the immunoblotting method described is more sensitive than immunofixation followed by silver staining. 相似文献
15.
Allele and genotype frequencies for the five PCR-based loci were analyzed in 157 unrelated Turkish individuals. The five PCR-based loci included LDLR, GYPA, HBGG, D7S8, and Gc. The results of the chi-square and exact tests showed that the genotype distribution at the LDLR, GYPA, D7S8, and Gc loci did not significantly differ from the Hardy-Weinberg Expectation (HWE). However, the genotype distribution at the HBGG locus did not conform to HWE. Moreover, the genotype frequencies calculated in this study were compared with the published genotype frequencies of US African American and US Caucasian populations. The Turkish population was significantly different at the HBGG locus from the US Caucasian population. However, there were highly significant differences at the LDLR, HBGG, and Gc loci between the Turkish and African American populations. 相似文献
16.
S A Westwood P J Seaman C O'Brien L J Thorogood 《Forensic science international》1987,35(2-3):197-207
The phenotypic frequencies of group-specific component (Gc) and alpha-2-HS-glycoprotein (A2HS) were determined in White European, Asian and Afro-Caribbean populations. Typical allele frequencies were observed for Gc, with Gc 1S being the major allele for the first two groups and Gc 1F being the major allele for Afro-Caribbeans. For all groups the dominant A2HS allele was A2HS 1, although Asians had a significantly higher proportion of this allele than the White Europeans. Gc and A2HS either singly or in combination with other blood grouping systems provide good discriminating potential. The A2HS 10 allele was detected with a very low frequency in the White European group (A2HS 10 = 0.0013) and was not detected in the Asian group, while the Afro-Caribbean group had a relatively high frequency of this allele (A2HS 10 = 0.0966). The different distribution of the Gc 1F and A2HS 10 alleles in White Europeans and Asians compared with Afro-Caribbeans, can be used to determine the likelihood of blood coming from an Afro-Caribbean. 相似文献
17.
B Budowle 《Forensic science international》1987,33(3):187-196
A method is described for subtyping group-specific component (Gc) derived from human bloodstains. Bloodstained cuttings were extracted in 6 M urea. The extracts were subjected to ultrathin-layer polyacrylamide gel isoelectric focusing in the pH 4.5-5.4 range. After isoelectric focusing, Gc was detected by immunofixation in cellulose acetate membranes. This method permitted the successful typing of Gc in at least four-month-old bloodstains maintained at room temperature. Bloodstains from 266 liquid blood samples of known origin were subjected to both this method and immunofixation conventional agarose gel electrophoresis with no phenotypic discrepancies observed. The Gc population data for Whites from Baltimore, Maryland, were homogeneous with white sample populations from other geographical locations within the U.S.A.; while Gc data from northern U.S.A. black sample populations appeared to be heterogeneous compared with a southern United States black sample population. 相似文献