成都地区汉族Gc亚型的分布及血痕中Gc亚型的检测

作者用免疫固定薄层聚丙烯酰胺凝胶等电聚焦(PAGIF)技术,调查了成都地区无关的125名健康汉族人血清Gc亚型分布。其6种亚型频率(%)分别为:Ge1F=20.8,Ge1S=8.0,Gc1F-1S=18.4,Gc2-1F=30.4,Gc2-1S=16.0和Gc2=6.4。Gc的基因频率为:Cc~(1F)=0.452,Gc~(1S)=0.252和Gc~2=0.296。对保存于室温条件下20周的陈旧血痕进行了Gc亚型定型,获得满意结果。

Gc SUBTYPING IN THE HAN POPULATION IN CHENGDU AND PHENOTYPING OF Gc IN HUMAN BLOODSTAINS

We succesfully examined the Gc subtypes both in the human sera and in the dried bloodstains using PAGIE(pH4～6)followed by immunofixation method with rabbit anti-human Gc antiserum.Gc phenotyping of 125 serum samples from unrelated Chinese blood donors in Chengdu were carried out.The dis- tribution of six Gc subtypes were:Gc1F 20.8%;Gc1S 8.0%;Gc1F—1S 18.4%; Gc2—1F 38.4%;Gc2-1S 16.0%;Gc2 6.4%.The gene frequencies were Gc~(1F) 0.452;Gc~(1S) 0.252;Gc~2 0.296 respectively.The observed and expected values provided a good fit to the Hardy—Weinberg equilibrium(P>0.05).The exclusion probability of Gc is 34.8% and discrimination probability of Gc is 79.44%.There are not any significant difference of the distribution of Gc subtypes between the Hun population in Chengdu and those in Hong Kong and Japanese.The difference of the distribution of Gc subtypes between the Han population in Chengdu and those in Malaysia,Indonesia,India as well as the American caucasians,Belgians,Icelander and West German are sig- nificant. The phenotyping of Gc in 11 bloodstain samples kept in room temperature for twenty weeks were carried out successfully also using PAGIF followed by immunofixation method.