The use of the Foster & Freeman Fx5 Fibre Finder in forensic textile examinations.

The Fx5 Fibre Finder has been developed by Foster & Freeman Ltd to automatically search tape lifts for coloured fibres specified by the operator. Experiments and casework studies have been conducted to assess primarily the performance of the instrument but also, where possible, to compare it with manual searching. Tape lifts have always been manually searched at this laboratory and it is a laborious task. The use of a machine such as the Fx5 would release a scientist from many hours of low power microscopy to be free for other duties and therefore could be a valuable labour saving device in forensic textile examination.     

Is it a modacrylic fibre?

This study describes the characteristics of modacrylic fibres and includes over 80 samples (previous and current) representing 15 trade names. Fibre morphology was examined using brightfield microscopy. Signs of elongation were determined using polarised light microscopy. Fibre cross sections were also examined. The generic class of fibre was divided into sub groups using polymer composition as determined by FTIR-microscopy. Microscopically, some modacrylic fibres cannot be distinguished from acrylic fibres. Others display unusual optical and morphological features which are a strong indication of their generic class. The infrared spectra provide information about the co-monomer, termonomers added to produced dye sites, the presence of solvent residue, dyes, and additives, e.g. flame retardant material. The infrared spectra should always be recorded before and after any thin layer chromatographic examination of the dye, otherwise peaks attributable to dyes, which may be a valuable comparative feature in casework will be lost.     

The transfer and persistence of automotive carpet fibres on shoe soles

The transfer and persistence of automotive carpet fibres to shoe soles was investigated. It was found that fibres were transferred with the normal activity of a car passenger. Carpet type and shoe sole parameters were significant determinants in the number of fibres that transferred. The average number of fibres was between about one and 33 per sole. Fibres that had been transferred after normal activity only persisted for a few minutes after walking. A survey of the shoe soles of people about to leave their car showed that fibres were usually present. The majority of shoe soles surveyed had less than five fibres with the greatest number of fibres found being 14. The likelihood of finding a large number of fibres on such soles is rare. Fibre composition of automotive carpets showed a high degree of variation. Grey was seen to be a common colour irrespective of the colour of the vehicle body.     

Potentiality of 3D laser profilometry to determine the sequence of homogenous crossing lines on questioned documents

The determination of the sequence of line crossings is still a current problem in the field of forensic documents examination. Optical examination, lifting technique, ESDA technique, and electron microscopy are the most widely used methods for the determination of the writing order of crossing texts. However, at present many examinations of intersecting lines result in an inconclusive opinion, particularly if the same type and colour of ink is involved. This paper presents the potentiality of the 3D laser profilometry, which has been to determine the chronological sequence of homogenous "crossing lines". The laser profilometry, illustrated in this paper, has been developed on a conoscopic holography based system. It is a non-contact three-dimensional measuring system that allows producing holograms, even with incoherent light, with fringe periods that can be measured precisely to determine the exact distance to the point measured. This technique is suitable to obtain a 3D micro-topography with high resolution also on surfaces with unevenness reflectivity (usual for the paper surface). The proposed technique is able to obtained 3D profile in non-invading way. Therefore, the original draft are not physically or chemically modified, allowing a multi-analysis in different times. The experiments performed with line crossings database show that the proposed method is able of "positive identification" of writing sequence in the majority of the tests. In absence of a positive identification, the result has been "inconclusive" (no false determination did occur in this work).     

Isoelectric focusing of human hair keratins: correlation with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns and effect of cosmetic treatments.

A new isoelectric focusing (IEF) technique in polyacrylamide gels with 6M urea and 1.5% Nonidet P40 has been developed to characterize human hair samples. The phenotypes demonstrated with this procedure has been correlated with the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns described by other authors. The method described can be applied in the forensic science analysis of a single human hair. Using the same IEF technique we have studied the changes in electrophoretic patterns of cosmetically treated hair. The characteristics of the modifications observed and its utility in forensic science work are also discussed in this paper.     

The forensic analysis of soils and sediment taken from the cast of a footprint

The routine production of a cast of a shoe-print taken in soil provides information other than shoe size and gait. Material adhering to the surface of the cast represents the preservation of the moment of footprint impression. The analysis of the interface between the cast and soil is therefore a potentially lucrative source of information for forensic reconstruction. These principles are demonstrated with reference to a murder case which took place in the English Midlands. The cast of a footprint provided evidence of a two-way transfer of material between the sole of a boot and the soil of a recently ploughed field. Lumps of soil, which had dried on a boot, were deposited on the field as the footprints were made. Pollen analysis of these lumps of soil indicated that the perpetrator of the imprint had been standing recently in a nearby stream. Fibre analysis together with physical and chemical characteristics of the soil suggested a provenance for contamination of this mud prior to deposition of the footprint. Carbon/nitrogen ratios of the water taken from the cast showed that distilled water had been used thus excluding the possibility of contamination of the boot-soil interface. It was possible to reconstruct three phases of previous activity of the wearer of the boot prior to leaving the footprint in the field after the murder had taken place. This analysis shows the power of integrating different independent techniques in the analysis of hitherto unrecognised forensic materials.     

ORO. The Physical Developer replacement?

In the process of fingerprint development Physical Developer has been largely the method of choice on porous surfaces after coming into contact with wet environments. It is only recently that a new technique has been identified which could replace this standard technique. This study aims to build on previous research and expand knowledge regarding the technique. The study built on previous research and compared Physical Developer to Oil Red O, testing both on four paper types, while being placed in three different water types and an accelerant for various amounts of time. Marks were placed with both heavily ‘loaded’ sebaceous fingers and ‘normal’ un-washed fingers. Results show that Oil Red O consistently produced clearer more detailed marks from the ‘loaded’ fingers, but neither technique proved to work better on the ‘normal’ marks. Neither technique developed any prints from the accelerant.     

The importance of thin layer chromatography and UV microspectrophotometry in the analysis of reactive dyes released from wool and cotton fibers

Samples of reactively-dyed wool and cotton were obtained from a range of dye manufacturers, dye distributors and the Forensic Science Service (FSS) Fibre Data Collection. The wool fibers were red in color and had previously been compared using comparison microscopy (CM), visible range microspectrophotometry (VS) and thin layer chromatography (TLC). The cotton fibers were blue and black in color and had not been previously compared. Red, blue and black fibers were chosen because they are often encountered in casework. The usage of reactive dyes to color fibers has increased over the last 10-15 years and these are often seen in casework. Before techniques were available that allowed reactively-dyed fibers to be compared using TLC only CM and microspectrophotometry were routinely carried out. Many laboratories, who had a microspectrophotometer, only had a visible range instrument. It was therefore important to see which techniques provide additional information, that gives greater individuality to fibers, to that obtained from CM. The color was released from the wool and cotton fibres using alkaline hydrolysis and a cellulase enzyme respectively. Many of the red wool samples were differentiated from each other using CM. More differentiation was found using VS and even more when ultraviolet range microspectrophotometry (UV) or TLC was used. Two samples could only be differentiated using TLC because CM, VS and UV failed to separate them. The black cotton samples were predominately differentiated using CM but VS allowed for further differentiation. With the samples used in this project UV and TLC failed to separate the samples further. The blue cotton samples benefited from the use of CM, VS and either UV or TLC to reduce the number of matching pairs. All techniques aided differentiation although with this set TLC and UV proved to be complementary techniques. Results demonstrate that TLC and UV both yield important information over and above that obtained from CM and VS. Although in some parts of the project TLC and UV are complementary if the concentration of the dye in the fiber is not sufficient for TLC or the scientist doesn't wish to 'destroy' the fiber UV would be of more use than TLC.     

Correlation of age and incremental lines in the cementum of human teeth

It has been long recognized that cementum thickness increases with age. Much literature has recently been devoted to utilizing incremental lines in cementum as an aging criterion in animals, but only one study has been done suggesting this technique in humans. Thirty-one teeth of known age were histologically stained and sectioned to observe incremental lines. Direct predictions of age based on these lines underestimated the age of older specimens. However, there was a correlation between number of lines and age, and with a large enough specimen size, a computer-generated formula for age prediction may be possible.     

Photographs of the human face and broken projective symmetry

The exact projective symmetry manifested in photographs is broken if the object is not flat. It has been found that front view perspectives of the human face, photographed with limited variations in camera angle (within 30 degrees) show a breaking projective symmetry sufficiently small to be considered for accurate comparison. As a practical implementation of this new approach, a computer-controlled video camera superimposition technique has been developed and demonstrated in the identification of a disguised human face.     

DNA分析技术在法科学中的应用及展望

本综述对DNA指纹、PCR -VNTR、PCR -STR、PCR -mtDNA测序等技术的发展 ,以及其在法科学中的应用领域和发展前景作了系统的阐述。认为由于DNA分析技术所具有的特点 ,使之已成为现今法科学生物检材检验的主要手段之一。阐述了现阶段DNA分析技术已向标准化、自动化和高鉴别机率方向发展 ,以及建立DNA罪犯数据库的必要性和应用价值     

Interpreting small quantities of DNA: the hierarchy of propositions and the use of Bayesian networks

The dramatic increase in the sensitivity of DNA profiling systems that has occurred over recent years has led to the need to address a wider range of interpretational problems in forensic science. The issues surrounding questions of the kind "whose DNA is this?" have been the subject of considerable controversy but now it is clear that the emphasis is shifting to questions of the kind "how did this DNA get here?" Such issues are discussed in this paper and new insights are provided by two particular recent developments. First, the notion of the "hierarchy of propositions" that has arisen from a project called Case Assessment and Interpretation (CAI) that has been running in the British Forensic Science Service (FSS). Second, a technique for drawing inferences in the face of many interacting considerations, known as "Bayesian networks"--or "Bayes' nets" for short--that has been the subject of an earlier paper in this journal (1). The discussion is carried out by means of case studies, based on actual cases. It is clear that, whereas the inference in relation to the source of the DNA in a crime sample might be overwhelmingly strong, the inference in relation to the propositions that a jury must consider relating to the identity of the actual offender may be much more tentative.     

The application of infrared chemical imaging to the detection and enhancement of latent fingerprints: method optimization and further findings

Fourier transform infrared (FTIR) chemical imaging allows the collection of fingerprint images from backgrounds that have traditionally posed problems for conventional fingerprint detection methods. In this work, the suitability of this technique for the imaging of fingerprints on a wider range of difficult surfaces (including polymer banknotes, various types of paper, and aluminum drink cans) has been tested. For each new surface, a systematic methodology was employed to optimize settings such as spectral resolution, number of scans, and pixel aggregation in order to reduce collection time and file-size without compromising spatial resolution and the quality of the final fingerprint image. The imaging of cyanoacrylate-fumed fingerprints on polymer banknotes has been improved, with shorter collection times for larger image areas. One-month-old fingerprints on polymer banknotes have been successfully fumed and imaged. It was also found that FTIR chemical imaging gives high quality images of cyanoacrylate-fumed fingerprints on aluminum drink cans, regardless of the printed background. Although visible and UV light sources do not yield fingerprint images of the same quality on difficult, nonporous backgrounds, in many cases they can be used to locate a fingerprint prior to higher quality imaging by the FTIR technique. Attempts to acquire FTIR images of fingerprints on paper-based porous surfaces that had been treated with established reagents such as ninhydrin were all unsuccessful due to the swamping effect of the cellulose constituents of the paper.     

CT技术的应用对鼻区骨折法医学鉴定意见的影响

目的 探讨CT技术的应用对鼻区骨折法医学鉴定的影响,发现现行鉴定标准存在的问题提出修订意见.方法 运用x2检验比较两个不同时期分别以X线和CT为主要鉴定依据评定为轻伤的案件,探究医学放射影像检查技术对鼻区骨折鉴定意见的影响程度.结果 发现CT技术对鼻区骨折的鉴定结果的影响,与X线比较,其差异有统计学意义,并暴露出基于X线影像技术的鼻骨损伤鉴定标准条款不再适应目前鉴定实践状况的问题.结论 在CT技术广泛应用后,现行的鼻骨损伤鉴定标准条款不能很好地适应医学发展,有必要重新制定基于CT技术的鼻区骨折鉴定标准.     

Investigation into the usefulness of DNA profiling of earprints

DNA profiling of biological trace evidence has been used for many years. The application of this technique specifically to the DNA profiling of earprints has not to date been thoroughly investigated. This report presents the results of 60 earprints collected from three healthy adult volunteers under controlled laboratory conditions. DNA profile analysis revealed that high levels of non-donor alleles are observed when earprints are collected for DNA profiling. The source of these non-donor alleles is investigated and the impact that their presence within the profile may have on the use of this technique is discussed.     

Scanning memory with Yara

Memory analysis has been successfully utilized to detect malware in many high profile cases. The use of signature scanning to detect malicious tools is becoming an effective triaging and first response technique. In particular, the Yara library and scanner has emerged as the defacto standard in malware signature scanning for files, and there are many open source repositories of yara rules. Previous attempts to incorporate yara scanning in memory analysis yielded mixed results. This paper examines the differences between applying Yara signatures on files and in memory and how yara signatures can be developed to effectively search for malware in memory. For the first time we document a technique to identify the process owner of a physical page using the Windows PFN database. We use this to develop a context aware Yara scanning engine which can scan all processes simultaneously using a single pass over the physical image.     

Provenance determination of oriental porcelain using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS)

The sale of fraudulent oriental ceramics constitutes a large proportion of the illegal artifact and antique trade and threatens to undermine the legitimate international market. The sophistication and skill of forgers has reached a level where, using traditional appraisal by eye and hand, even the most experienced specialist is often unable to distinguish between a genuine and fraudulent piece. In addition, current provenancing techniques such as energy-dispersive X-ray fluorescence (EDXRF) spectrometry and thermoluminescence (TL) dating can result in significant damage to the artifact itself. Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), a relatively nondestructive analytical technique, has been used for the provenance determination of materials based on geographical origin. The technique requires the production of a laser crater, c. 100 microm in diameter, which is essentially invisible to the naked eye. Debris from this crater is analyzed using ICP-MS, with the results forming the basis of the provenance establishment protocol. Chinese, Japanese, and English porcelain shards have been analyzed using this protocol and generic isotopic distribution patterns have been produced that enable the provenance establishment of porcelain artifacts to their country of production. Minor variations between elemental fingerprints of artifacts produced in the same country also indicate that it may be possible to further provenance oriental ceramics to a specific production region or kiln site.     

Sex differences and aging of the vertebral column

Morphological changes in the adult human skeleton have been recognized as useful for estimating the age at death. In the vertebral column, the development of osteophytes has been shown to be a general indicator of age, although substantial variation has been documented. The technique used for estimating age from osteophyte development is based exclusively on males and it is unknown whether patterns of osteophyte development are comparable between the sexes. This study examines sex differences in osteophyte development in the thoracic and lumbar regions of 384 individuals from the Terry Collection. Males and females in this sample show remarkably similar patterns of age-related changes in osteophyte development; however, females show greater variability in osteophyte stage for a given age. This was confirmed with age-matching a subsample of 128 individuals. Therefore, slightly larger confidence intervals should be used when assessing age from the vertebral column in females.     

ASTM standards for fire debris analysis: a review

The American Society for Testing and Materials (ASTM) recently updated its standards E 1387 and E 1618 for the analysis of fire debris. The changes in the classification of ignitable liquids are presented in this review. Furthermore, a new standard on extraction of fire debris with solid phase microextraction (SPME) was released. Advantages and drawbacks of this technique are presented and discussed. Also, the standard on cleanup by acid stripping has not been reapproved.Fire debris analysts that use the standards should be aware of these changes.     

The detection and analysis of ignitable liquid residues extracted from human skin using SPME/GC

A simple, fast, inexpensive, and sensitive technique for the detection and identification of flammable or combustible liquid residues on the skin of arson suspects is presented. The use of solid phase microextraction (SPME) for the analysis of ignitable liquid residues has been demonstrated and it is shown in this work that this technique is effective in extracting these liquid residues at extremely low quantities. Microliter quantities of controlled spikes of gasoline, diesel fuel, and charcoal lighter fluid were deposited on the hands of a volunteer and extracted after several time intervals. The SPME technique can recover very small amounts of liquid deposits on skin up to 3.5 h after exposure, depending on the class of the ignitable liquid residue used.