猪瘟野毒E2基因同源性比较

以人工感染发病猪的脾为材料提取总RNA ,根据已报道的猪瘟病毒基因组序列 ,设计合成了 2对引物 ,以总RNA为模板 ,利用反转录聚合酶链式反应 (RT PCR)、套组聚合酶链式反应(nPCR)及测序技术 ,对流行于我国甘肃省、陕西省、宁夏和广西自治区的 5株野毒株的主要外膜糖蛋白E2基因的核苷酸序列进行了测定 ,用DNAstar软件比较分析了 5株野毒之间及其与猪瘟兔化弱毒株 (C ST株 )E2基因的核苷酸序列和推导的氨基酸序列的同源性。结果发现 ,5株野毒与C ST株核苷酸序列的同源性为 81.7%～ 83.1% ,氨基酸同源性为 87.3%～ 88.6 % ,表明它们之间存在较大的差异 ;但 5株野毒之间核苷酸序列的同源性高达 98.8%～ 99.3% ,氨基酸同源性为96 .6 %～ 99.2 % ,表明它们之间的差异较小     

论非法进行节育手术罪的主体及其立法完善

关于非法进行节育手术罪的主体问题,刑法理论界一直存在争议。我们认为,刑法设置 非法进行节育手术罪的目的主要是为了防范和打击各类严重破坏国家计划生育立法、危害妇女生 命健康的犯罪行为。以此为基点,对非法进行节育手术罪的主体,我们应当做宽泛的理解。我国现 行刑法对非法进行节育手术罪主体的规定存在着明显缺陷,在今后我国的刑事立法过程中,应考虑 修改刑法对非法进行节育手术罪的规定。     

环境法与主流法的规范衔接和协调问题

环境法规范分为基础性规范和派生性规范 ,其效力要么来源于主流法规范 ,要么能被主流法的价值目标所阐释。为了适应不断发展的客观形势 ,解决现实的环境问题 ,基于调整和保护对象的共同性或相关性及在调整机制方面的同一性或衔接性 ,环境法规范需要在技术层面甚至原理层面与主流法规范通过派生或阐释的方式衔接和协调。这两个层面的派生或阐释工作不仅促进了主流法规范的丰富和发展 ,还夯实了环境部门法的独立基础。     

跨文化传播时代的广告

广告文化作为文化传播的一种形式,必须为弘扬民族文化、实现民族文化的合理拓展作出承诺。从广告传播的角度来看,广告若欲将信息传递给目标受众,就必须采用本民族容易接受的文化形式和文化价值观。因此,在广告传播中采用民族题材、弘扬民族文化价值观的广告作品十分多见。这些广告表现了同一文化的亲和力,极大的唤起了消费者的文化认同,从而导致其购买行为,而客观上它们又弘扬了民族传统。     

论林业物权客体的确定——中国法律经验的总结与评析

我国林业法律中的林业物权客体相当宽泛，包括森林资源、森林、林木、林地、野生动物、野生植物等，但根据物权法的原理和立法，它们并不完全符合物权的特定性等要求。根据物权客体的标准和类型，林业物权的客体宜限定为林木、林地、野生动植物。     

国外老年服务一瞥

联合国秘书长安南说过:"我们目前所生活的时代,人们赋予它各种各样的名称……今天,请允许我再增加一个名称,即:长寿时代."在这样一个长寿的时代里,人生70已不再是古来稀.为更好地应对老龄化挑战,增强老龄化社会的关注意识,推动个人晚年生活的成功,现代化社会赋予了老年人以更多的生活选择,各类养老机构的出现代表着养老社会化的趋势.近年来,国外一些地区为了提高老年人的生活质量,使他们愉快地安度晚年,开办了各种有趣的老年服务项目.在此,我们作以简单介绍.     

Characterization of the N+3 stutter product in the trinucleotide repeat locus DYS392

Stutter products generated during DNA amplification by the polymerase chain reaction (PCR) may complicate mixture interpretation. The PCR amplification of the DYS392 locus typically results in three distinct detectable PCR products: the true allele product (N), a stutter product three bases smaller (N-3), and a reproducible low-level product, three bases larger (N+3). Sequence analysis of the N+3 product demonstrated that its sequence is one TAT repeat longer than the true allele product. Our experiments demonstrated that the quantity of both N-3 and N+3 stutter increased as the allele number increased. The percent stutter also increased as the magnesium concentration was increased in the reaction, as well as when the amount of input DNA was decreased. As both stutter products behave in a similar and reproducible fashion, the same rules that apply to the interpretation of N-3 stutter products in short tandem repeat analysis, can be applied to N+3 stutters. The characterization of the DYS392 N+3 product is the first detailed published study of a stutter product larger than the true allele.     

活体鉴定400例放射学特点分析──兼论法医临床学与放射学的关系

为探讨法医临床学鉴定与放射学的关系，本文对４００例活体鉴定就放射学检查的有关问题进行回顾性研究。结果表明：２６６例进行了放射学检查（占６６．５％），其中Ｘ线１７６例（６６．２％），ＣＴ４３例（１６．１％），两者均有的４７例（１７．７％）。放射学检查部位中，Ｘ线以四肢为多，ＣＴ以颅脑为多。误诊率为９．０２％，放射学检查与致伤原因之间有一定关系，轻微伤中不必要的ＣＴ检查多见。并就放射学误诊的法律后果及不必要的放射学检查问题进行了讨论。本文从法医临床学的角度对放射学进行了评价，指出在活体鉴定中可以应用放射学知识协助证明损伤、推断损伤形成机制、个人识别及研究损伤时间。因此法医学鉴定人必须有放射学理论和技术方面的知识。作者还试图提出法医临床放射学。     

Possibilities of the positive following violence and trauma: informing the coming decade of research

The effects of trauma and violence may be better understood by taking a broader perspective that includes resilience and recovery as well as damage and symptomatology. Based on this broader view, this article describes three interrelated, cutting-edge trends in mental health research: (a) the positive psychology movement, (b) the recognition of the role of spirituality and religion in health and well-being, and (c) stress-related growth. The integration of these trends into mainstream studies of trauma and violence will provide a counterbalance to the predominant orientation of victimization and pathology currently evidenced in the literature. All three have important implications for survivors of violence and trauma.     

Determining the Refractive Index Variation within Panes of Vehicle Windshield Glass

Refractive indices of seven double‐paned vehicle windshields were measured to assess the variation across the pane of glass and to evaluate collection techniques for known glass standards by comparing false negative rates. Measurements were made using a Foster and Freeman GRIM3 instrument, and a minimum of 240 measurements were made per pane. The mean SD of the windshields was 0.00004. It was further determined that collecting a known sample from two different sections of a shattered windshield gave the lowest rate of false negatives when using ± 2 standard deviations to estimate the RI variation of the known glass. Additionally, refractive indices often were highest in the center of the windshield and decreased toward the left and right edges; in approximately half the sample set, the two panes of a single windshield were differentiated by one or more properties.