Developmental Validation of the Quantifiler® Duo DNA Quantification Kit for Simultaneous Quantification of Total Human and Human Male DNA and Detection of PCR Inhibitors in Biological Samples*

Abstract: The Quantifiler^® Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. Pooled human male genomic DNA is used to generate standard curves for both human (ribonuclease P RNA component H1) and human male (sex determining region Y) specific targets. A shift in the cycle threshold (C_T) values for the internal positive control monitors the presence of PCR inhibitors in a sample. The assay is human specific and exhibits a high dynamic range from 0.023 to 50 ng/μL. In addition, the multiplex assay can detect as little as 25 pg/μL of human male DNA in the presence of a 1000-fold excess of human female DNA. The multiplex assay provides assessment of the DNA extract and guidance for the selection of the appropriate AmpFℓSTR^® Amplification Kit to obtain interpretable short tandem repeat profiles.     

政府数字化转型影响因素分析

我国正处于推进国家治理现代化和新一代信息技术革命的交汇期,推动政府数字化转型是实现国家治理体系和治理能力现代化的必然要求.本文借鉴既有研究,借助TOE框架,运用层次分析法和模糊综合评价法,从技术、组织以及环境层面对我国政府数字化转型的影响因素进行了分析和评价.结果 显示,政府数字化转型战略规划是推动政府数字化转型进程最...     

Test of chlorine wipes for efficient removal of DNA from forensic genetics laboratories

Sodium hypochlorite is an efficient reagent for removal of unwanted DNA from laboratory surfaces. Here, we tested two different chlorine wipes and compared their performance to a 0.9–1.8% hypochlorite solution. WipeClean Chlorine Disinfection wipes contain > 0.1 g sodium hypochlorite/kg, whereas WetWipe Chlorine Desinfection wipes contain > 1000 ppm active chlorine. Clean surfaces were contaminated with 10 µL 0.5 ng/µL of massively parallel sequencing libraries. The DNA was dried and left for 45 min before any treatment. The surfaces were cleaned using either 1) a 0.9–1.8% hypochlorite solution and clean wipes, 2) a WipeClean wipe, 3) a WetWipe, or 4) the surface was not cleaned. All experiments were repeated three times. Subsequently, the surfaces were swabbed using cotton swabs. DNA was extracted from the swabs and the DNA concentrations were determined in quadruplicates by real-time PCR. This protocol was repeated after the soft plastic wrapping around the wipes were left open or closed for several weeks. The results showed that the WipeClean wipes efficiently removed DNA for up to four weeks after the box with the wipes were opened, whereas the WetWipe wipes dried faster and gradually lost their cleaning effect.     

美国自由贸易协定中数字产品贸易的规制研究

在WTO关于数字产品贸易的谈判中,美国一直主张适用GATT和零关税,但遭到了欧盟等的坚决反对。之后,美国通过自由贸易协定的形式推行其政策立场。在自由贸易协定中设立＂电子商务章＂,以有别于货物贸易和服务贸易;在跨境服务章中采用限制性清单的方法,力争为数字产品贸易提供较大程度的自由化。美国通过自由贸易协定规制数字产品的模式尽管值得商榷,但确实为WTO规制数字产品贸易进行了先行的尝试,拓展了其国内政策的国际空间。美国这种竭力将其国内政策推行为国际共同立场的做法,对于经常主张与国际接轨并被动接受国际规则的中国来说,无疑值得借鉴。     

利用aroA基因建立鸡传染性鼻炎PCR诊断方法

利用aroA基因设计了1对引物,分别对10株标准副鸡嗜血杆菌(HPG)菌株、14株分离的HPG菌株进行PCR扩增,结果均得到了与预期大小一致的片段,而对10株非HPG菌株和3株病毒进行扩增则无相应片段产生;该PCR能检测出10个菌细胞.与常规PCR方法相比,aroA-PCR的敏感性更高.     

数字政府发展的国际新趋势：理论预判和评估引领的综合

＂数字政府＂是同数字时代相适应的公共治理方式与形态。总体上讲,研究国际上数字政府发展趋势的路径有三种,即理论预判、评估引领和实践梳理。本文综合运用理论预判和评估引领两种路径,从技术逻辑、管理逻辑和政策逻辑三个层面对数字政府的新趋势进行提炼和总结。     

多路数码管显示仿真系统懿设计与实现

文章介绍了基于单点单路I／O仿真系统的多路数码显示仿真实现的分析与设计。     

电子商务立法刍议

我国的电子商务立法 ,可先采用条例形式 ,在今后实践中再充实完善。电子商务法应设置总则 ;未满十周岁的儿童不能成为电子商务的主体 ,所产生的法律后果由监护人承担 ;认证机构应具有电子商务的主体资格。对签署、证据、成立等行为应作出效力规定 ;各级工商行政管理局是电子商务的行政管理机关 ,各级邮电管理局也应成为管理机关。当事人在交易中违反民事法律 (规 )、合同约定的 ,均应承担民事责任。     

数字图书馆初探

数字图书馆是21世纪全球文化科技竞争的焦点之一,发展数字图书馆对于一个国家的经济和社会发展具有重要意义.数字图书馆具有众多说法,无一定论.数字图书馆与传统图书馆具有非常大的差别,它具有独特的特征、体系结构、工作模式.目前,数字图书馆还存在许多急需解决的难题,如技术、版权等.     

数字图像处理技术的优势

随着计算机应用软件多种类的开发 ,利用计算机来处理图像已成现实。计算机图像处理技术(也称数字图像处理技术)相对于传统的暗房技术 ,不但具备传统暗房技术制作影像的所有的技巧 ,而且还有许多传统暗房技术不可比拟的优势。数字图像处理技术是摄影艺术再创造的技术 ,可制作完美无暇的照片 ,照片质量比传统暗房技术制作的质量更高,对环境无污染 ,出片速度快、省时、省财、省力、省地方。数字图像处理技术必将取代传统的暗房技术