运用二代测序技术拆分混合STR分型

目的探讨二代测序技术在混合STR分型拆分中的应用。方法对一例强制猥亵妇女案中受害人颈部的拭子和血样作DNA提取,以Precision ID GlobalFiler^TM NGS STR Panel v2试剂盒制备文库,经Ion S5测序仪测序,运用Torrent_Suite_v5.2.1软件进行数据分析后,将检测基因座的序列多态STR分型与长度多态STR分型进行比较。结果在D8S1179、D21S11、D2S441、D2S1338、D10S1248五个基因座发现存在序列特异的等位基因亚型,利用这些亚型对混合STR分型进行了成功拆分。结论二代测序技术提供的等位基因序列信息可对混合STR分型的拆分起到帮助作用。     

Belarusians between East and West

Belarus has a divided identity that reflects its complex history and culture. A mixed-methods investigation incorporating focus groups and national representative surveys conducted over a decade or more suggests that Belarusians themselves are more likely to regard themselves as “European” than their counterparts in Ukraine and Russia, but less likely to do so than in other European countries. There is substantial support for a hypothetical European Union membership, particularly among younger respondents, but there is also strong and widely distributed support for a closer association with the other members of the Commonwealth of Independent States. Consistently, it is the “multidirectional” foreign policy promoted by the current leadership, which seeks closer relations with East and West at the same time, that finds the greatest support. But a “Slavic choice” is also popular, and much more so than a “Western choice” or isolationism.     

A Social Movement Theory Typology of Militant Organisations: Contextualising Terrorism

Typologies are ubiquitous in terrorism studies, illustrating their continued appeal as a tool to further our understanding of this form of political violence. Despite this, to date, the promise of an empirically derived typology has largely been neglected. In addressing this gap, this article sets out a typology developed from Social Movement Theory. Using a novel statistical technique to derive a three-dimensional framework for categorising militant groups, the typology incorporates both organisational characteristics and the wider political context. The result is a typology defined by three conceptual constructs: political capacity, war-making capacity, and network capacity. Alongside these organisational features, imposing measures of the wider political opportunity structure reveals eight types of militant organisation. To explore the utility of the framework, a preliminary analysis interprets the typology in light of the presence of wider conflict. That a robust relationship is found between the various types and whether groups were operating in peacetime, civil war, or low-intensity conflict, goes some way to demonstrating its utility as an analytical tool. Conclusions draw attention to the importance of contextualising militant groups in their socio-political setting, and the benefits of combining theory alongside empirical analysis to develop robust characterisations of violent organisations.     

The Importance of Improving Implementation Research for Successful Interventions and Adaptations

This special issue explores the theoretical underpinnings, triumphs, and challenges of implementing four early childhood education interventions. In doing so, each article highlights the importance of studying the implementation context as part of the evaluation process. This commentary reflects on the entire issue, ultimately arguing that future evaluations must continue to conduct—and improve on—implementation research. Specifically, to understand evaluation findings and scale up or adapt interventions effectively, researchers must examine implementation processes systematically, using both quantitative and qualitative methods. This includes: explaining how interventions were designed, theorizing the relationships between implementation processes and outcomes, defining the implementation phase under study, examining the validity and reliability of implementation measures, and using accessible language in reports.     

Sexual Harassment in the Norwegian Theatre World

ABSTRACT

The aim of this article is to provide insight into the phenomenon of sexual harassment in the theatre world. A survey among Norwegian actors is presented showing that sexual harassment is much more prevalent in the theatre world than in Norwegian work life in general. Further, the article aims at understanding why the prevalence is as high as it is. Based on qualitative interviews, the article points out some risk factors that shed light on the high prevalence of sexual harassment. Lastly, the risk factors are related to charismatic authority as an important power base in the theatre world.     

Choosing your own Boss: Variations of Representation Foci in Mixed Electoral Systems

Members of parliament are privileged agents. They can choose who to regard as their principal: the entire nation, a particular electoral district or a political party. Focusing on two countries with mixed electoral systems, Romania and Hungary, the article documents the dominance of the electoral logic of role-formation over the constraints of legislative organisation and the influence of socialisation. The focus of representation is found to be only modestly influenced by the degree of the embeddedness of MPs in political structures and hierarchies. The association of the seat with a particular territorial unit, on the other hand, has a robust effect even when this association originates in the political environment and not in formal rules. Finally, the psychological effect of losing a Single Member District is also found to influence how MPs perceive their representative role.     

Durable peace and sustainable development in Africa

Zimbabwean migration into South Africa represents a challenge to standard global models for responding to large-scale migration flows. South Africa's existing legal and institutional infrastructure for migration management and refugee protection is not adequate for responding to this challenge and yet the country has not planned or implemented alternative responses. The paper outlines the complex ‘mixed’ nature of current Zimbabwean migration to South Africa and describes how South Africa's range of legal and institutional responses are ineffective in protecting both South Africa's national interests and the rights of Zimbabwean migrants. After presenting a series of factors against which future responses should be evaluated, it lists various possible forms of response, including legal and humanitarian interventions, and discusses their respective appropriateness in the South African context. Finally, the paper suggests six possible policy scenarios, taking into account institutional and political realities on the ground in South Africa.     

混合STR分型分析方法研究进展

混合STR分型的解释及分析一直是国际上法医物证领域研究的热点和难点。随着DNA检验技术的发展,案件中检出的混合STR分型呈现出模板量降低以及混合组分数增加的趋势,其解释变得愈加复杂,传统的人工分析方法已难以满足现实要求。近几年国外基于统计算法模型的自动化软件解析方法渐趋成为混合STR分型分析的主要方法。本文综述了混合STR分型分析方法的相关研究进展,包括人工为主的定性分析方法以及基于统计模型的基因型概率分析方法;讨论了混合STR分型分析方法的未来发展趋势以及人工智能技术的应用前景。     

PCR-RFLP法检验混合斑中精斑ABO血型及基因型

运用PCR-RFLP检验方法,对21份精斑样品、5份混合斑检材进行了检验,结果表明该方法能够很好地检测混合斑中精斑的ABO血型及基因型,有效地解决了非分泌型精斑的ABO血型及基因型,拓宽了混合斑中精斑的检验范围.     

An electoral calculus? Dual incentives and committee assignment in the UK’s mixed-member legislatures

Although mixed-member electoral systems offer an apparent opportunity to observe how different rules shape politicians’ behaviour, ‘contamination’ between the SMD and PR-list tiers has frequently confounded academic work. Investigating Scotland and Wales’ mixed-member legislatures by exploiting their different chamber sizes and an unusual dual candidacy prohibition in Wales, modelling of committee assignments uncovers a split finding. Controlling for membership of the lead governing party, list members have a higher committee workload than their constituency colleagues, and members with previous employment experience in justice and health are more likely to be assigned to the corresponding subject committee once elected. Elsewhere, expectations that members might seek assignments that best suit theorized re-election interests are not found. The hypothesized influence of electoral rules is strongly conditioned by the small size of the legislature in Wales.