犬冠状病毒TN449株M基因的克隆与转移载体的构建

用蔗糖密度梯度离心纯化犬冠状病毒TN449株病毒液,提取总RNA并反转录,同时设计1对5′端加有BamHⅠ和HindⅢ内切酶位点的引物,对病毒cDNA进行了PCR扩增,回收PCR产物将其连接入pGEM-TEasy载体并转化大肠埃希氏菌。并对阳性重组质粒菌测序和序列分析。结果,犬冠状病毒与牛冠状病毒、猫冠状病毒、猫传染性腹膜炎病毒、人冠状病毒、鸡传染性支气管炎病毒、鼠传染性肝炎病毒、猪呼吸道冠状病毒、人重症急性呼吸道综合征病毒和猪胃肠炎病毒的同源性分别是39.39%、85.41%、83.98%、36.80%、26.64%、37.23%、93.51%、29.00%和94.81%;犬冠状病毒M蛋白有3个疏水性结构域。同时构建了pET28a-CCV-M表达质粒。     

讲史的文体形式及其在章回小说生成史上的重要作用

讲史是我国历史上的一种重要文学艺术形式.从文体角度而言,它一方面承继了史传的若干特点,另一方面又以民间的说书艺术点化了史传,使之向章回小说方向发生了决定性的转变.这些转变主要包括叙述者的现身、创作目的的由垂戒到娱人、创作方法的由实向虚以及故事形态的确立等.由于讲史以及这些特点的存在,史传向章回小说的过渡才得以完成.     

浅析网络小说中关于社会公德意识缺失的问题

网络小说作为当代网络文学中重要组成部分之一,其发展受到社会各界的关注,由于刚刚起步,发展中难免存在着各种严峻的问题,而当前发展中的一个重要问题既是网络小说中社会公德意识缺失问题,本着治病救人的原则,笔者着重分析了这种道德现象的成因以及解决问题的一些有益的思考,希望对解决此种问题有所帮助。     

长篇历史小说《孙中山》的纪实风格与艺术品位

长篇历史小说《孙中山》将在纪实基础上的合理想象与在艺术品位中遵循史实有机地结合起来,构成此部作品宏大气场。作品具有穿越时空的力量,将历史文献考据与合理的逻辑演绎精心对接,让历史人物及场景得以真实地再现。小说创作坚持严肃谨慎的态度,不断挖掘细节,丰富精神骨架。既重整体构思,也精细节刻画,注重艺术性和纪实性的高度统一。把历史巨人写成邻家少年,让他从神坛上真正走下来,走到读者中间,走到民众中间,给读者以历史时空的无限想像和智慧启迪。     

诗意红楼－－试论《红楼梦》的诗性本质

小说<红楼梦>是中国诗性思维在小说中的集中体现,与诗呈现出一种血缘的关系.<红楼梦>的叙事艺术中表现出来的抒情性、感伤气质和典雅风格,无不体现出与诗的内在一致性和诗化的美感追求.     

Dystopian Eugenics and Mestizo Futurisms in Eduardo Urzaiz's Eugenia

Set in 2218, the novel Eugenia envisions a contradictory utopian and dystopian future premised upon eugenical engineering. Here, I analyse Urzaiz's vision of the beginnings of eugenics in Mexico. Ultimately, I argue that Villautopia is presented as ever-vigilant and always responding to threats of degeneracy and social disorder, which accurately the reflect eugenicist underpinnings of Urzaiz's time. An analysis of Urzaiz himself and the Yucatán context is key as it envisions, translates and produces new eugenic ideas that deterritorialise eugenics and portrays it both in a ‘positive’ way while providing a series of ‘negative’ possibilities as a probable outcome.     

互动中的叙事模式--中国20世纪20年代雅俗小说形态研究

20世纪20年代中国雅俗小说在叙事模式方面存在较为明显的互动现象,具体表现在讲听模式与写读模式、情节模式与情调模式的转向、互动.叙事模式的互动不仅加速了通俗小说借鉴现代新小说的过程,促使了通俗小说由传统向现代的转变,而且促进了新小说的民族化叙事风格的形成,从而对现代小说格局的多元构建具有重要的价值和意义.     

n‐Ethyl Pentylone‐Related Deaths in Alabama

n‐Ethyl pentylone (NEP) is a chemical substance derived from cathinone. Synthetic cathinones are an evolving group of drugs with stimulating, mind‐altering effects sometimes referred to as novel or new psychoactive substances (NPS). There is scarce information in the medical literature regarding forensic cases in which NEP is detected in toxicological testing. We present four fatalities involving NEP from Alabama in 2017. Deaths were attributed to NEP toxicity in two cases (peripheral blood concentrations of 0.121 and 0.953 mg/L) and injuries caused by gunshot wounds in two cases (peripheral blood concentrations of 0.045 and 0.031 mg/L). One case involving NEP described an individual who exhibited classic CNS‐stimulant induced erratic behavior before being found dead. These cases enhance the forensic literature regarding specific NPS like NEP and provide contextual reference for professionals considering the significance of NEP in toxicological interpretation.     

网络小说对大学生思想政治教育的挑战及应对策略

网络小说在大学生中间的盛行,对大学生思想政治教育带来了巨大的挑战。它不仅极大地冲击了大学生正在形成的正确价值观念、思想观念,弱化了教育者的主导作用,而且也在一定程度上抵消了学校思想道德教育的作用。作为构成思想政治教育系统的要素,社会、学校、教育者和大学生,都应该发挥自己的作用,以实际行动来抵制网络小说对大学生的消极影响,推进网络时代大学生思想政治教育的新发展。     

Secret hideouts and the adolescent experience: Hubert on the art and politics of Adrian and the Tree of Secrets

The following interview examines Hubert's creative process for his graphic novel Adrian and the Tree of Secrets (Arsenal Pulp Press, 2014). It explores his thinking about gender and the adolescent experience in the context of a range of social issues including his own Catholic upbringing. This interview culminates with a discussion about homosexuality in contemporary France, Charlie Hebdo, and larger extremism.