Recovery of Environmental Human DNA by Insects*,†

Abstract: We tested the hypotheses that foraging insects can acquire human DNA from the environment and that insect‐delivered human DNA is of sufficient quantity and quality to permit standard forensic analyses. Houseflies, German cockroaches, and camel crickets were exposed to dusty surfaces and then assayed for human mitochondrial and nuclear loci by conventional and qPCR, and multiplex STR amplification. Over two experiments, 100% of insect groups and 94% of dust controls tested positive for human DNA. Of 177 individuals, 33–67% tested positive and 13 yielded quantifiable human DNA (mean = 0.022 ± 0.006 ng; mean dust control = 2.448 ± 0.960 ng); four had at least one positive allele call for one or more locus; eight others showed multiple peaks at some loci. Results imply that application to routine forensic casework is limited given current detection methodology yet demonstrate the potential use of insects as environmental samplers for human DNA.     

刑法上的因果关系探究

对刑法中的因果关系的判断应着眼于本国的司法实践。由于我国耦合式的犯罪构成模式,使得我国在刑法因果关系的判断上不可能仅是事实层面的判断,而应是事实层面和价值层面的统一。只有将这两个层面之间的关系理顺,才能对刑法上的因果关系做出合理的判断。     

3g运营商的法律风险分析——以3g产业链为视角

随着3g通信技术的深度发展,3g产业链呈现出多维互动格局.运营商将在更多环节、更广泛区域内与更多主体形成复杂和敏感的法律关系,从而增大了运营商所承担的法律风险.运营商必须强化对3g产业链的控制、协调、整合,合理有效地防范3g运营的法律风险.     

生产-流通关系协调发展：理论与对策研究

在流通产业对现代生产发展的意义日益加大的同时,我国传统流通产业发展滞后于生产发展的问题也日益暴露。模块化时代的到来,生产、流通环节的专业化分工要求有更多的协作,分工的协调效率受到更多的关注。目前我国生产-流通关系不协调,原因主要在于流通产业发展滞后于生产需要,借助于产业链整合实现流通产业的规模化发展,提升专业化流通渠道的效率功能,是中国产业系统健康发展的有效途径。      

犯罪心理测试反测试及其对策

犯罪心理测试技术是在甄别嫌疑人过程中使用的测谎方法.但由于测试中的原理机制等问题,反测试手段可以以多种方式对测过程和测试结果进行干扰.加强犯罪心理测试中的监控并提高反测试识别技术,可以较好地应对测试中出现的反测试.反测试的出现本身就是逃避测试的表现,一旦发现被测人存在反测试行为或心理,则应重点对其进行调查.随着新技术的发展,脑电测试结合测谎范式进行检测有了一定的研究基础,为今后的测谎方式提供了更多的选择.     

Ultrafast Amplification of DNA on Plastic Microdevices for Forensic Short Tandem Repeat Analysis

The majority of microfluidic devices used as a platform for low‐cost, rapid DNA analysis are glass devices; however, microchip fabrication in glass is costly and laborious, enhancing the interest in polymeric substrates, such as poly (methyl methacrylate) (PMMA), as an inexpensive alternative. Here, we report amplification in PMMA polymerase chain reaction (PCR) microchips providing full short tandem repeat profiles (16 of 16 loci) in 30–40 min, with peak height ratios and stutter percentages that meet literature threshold requirements. In addition, partial profiles (15 of 16 loci) were generated using an ultrafast PCR method in 17.1 min, representing a ~10‐fold reduction in reaction time as compared to current amplification methods. Finally, a multichamber device was demonstrated to simultaneously amplify one positive, one negative, and five individual samples in 39 min. Although there were instances of loci dropout, this device represents a first step toward a microfluidic system capable of amplifying more than one sample simultaneously.     

High‐Throughput STR Analysis for DNA Database Using Direct PCR,

Since the Korean criminal DNA database was launched in 2010, we have focused on establishing an automated DNA database profiling system that analyzes short tandem repeat loci in a high‐throughput and cost‐effective manner. We established a DNA database profiling system without DNA purification using a direct PCR buffer system. The quality of direct PCR procedures was compared with that of conventional PCR system under their respective optimized conditions. The results revealed not only perfect concordance but also an excellent PCR success rate, good electropherogram quality, and an optimal intra/inter‐loci peak height ratio. In particular, the proportion of DNA extraction required due to direct PCR failure could be minimized to <3%. In conclusion, the newly developed direct PCR system can be adopted for automated DNA database profiling systems to replace or supplement conventional PCR system in a time‐ and cost‐saving manner.     

A Molecular Key for the Identification of Blow Flies in Southeastern Nebraska*

Abstract: Immature blow flies (Calliphoridae) are typically the first colonizers of cadavers. Identification of the early instars using traditional, morphology‐based keys is difficult because of their small size, similarity, and simplicity in external morphology. Information derived from molecular genetic data would augment the accurate identification of immature flies. Nine species of blow flies commonly found in southeastern Nebraska were used to examine the utility of molecular‐based keys. Polymerase chain reaction–restriction fragment length polymorphisms (PCR–RFLP) were investigated with 10 common, inexpensive, restriction enzymes from an amplicon of approximately 1500 bp spanning the mitochondrial cytochrome oxidase I gene. A simple molecular taxonomic key, comprising RFLP from the restriction enzymes HinfI and DraI, enabled the differentiation of all species used. Further development of PCR–RFLP, including more extensive and intensive examination of blow flies, would benefit forensic laboratories in the accurate identification of evidence consisting of immature blow flies.     

从运动生化视角谈基层民警力量训练

基层民警力量训练存在着训练意识不强、训练方法不科学、重技能轻体能、健身器械和场馆不足等问题,应该从运动生物化学的角度出发,运用运动生物化学反应基本原理指导警察力量训练,遵循运用生物化学基本原理进行力量训练原则,依据运动生物化学原理进行力量训练,以提高基层民警力量训练的实效。     

易扶安置区风险链:易地扶贫搬迁安置区社会稳定风险的一个解释框架

易地扶贫搬迁初期,政府和移民对贫困认知存在分歧,搬迁便意味着风险环境酝酿。生活过渡期,由于政府扶持政策的脱节,移民的生计存在可持续困境,加之被激活的发展渴求,风险便被移民感知,谨慎抵抗抑或自助便成常态。而在社会放大机制下,这些不稳定要素便有了酿成社会稳定风险的可能,表现出“风险环境—风险感知—风险行为—社会放大—社会稳定风险”的风险传导链特征,呈现出双向传递性、集聚性和扩散性。如此,为实现安置区的稳定发展,应加快构建易扶安置区社会稳定风险预警指标体系,做好风险评估,并有针对性地探索形成基于安置区特征的有效治理路径。