Internal Validation of Human Mitochondrial DNA Quantification Using Real‐Time PCR

The quantity of mitochondrial DNA (mtDNA) template added for amplification and subsequent dye terminator reactions is critical for obtaining quality sequence data. Validation of a human mtDNA real‐time quantitative PCR (qPCR) assay demonstrated its high degree of reproducibility and precision as well as an extremely sensitive threshold of detection (0.0001 pg/μL or approximately six human mtDNA copies/μL). A study of 35 nonprobative bone and teeth evidence samples revealed that 20 pg of mtDNA template is recommended for successful HV1 and HV2 sequence analysis; however, as little as 0.013 pg can generate a full mtDNA profile when using enhanced amplification reactions. The assay can also detect PCR inhibition and is useful for identifying samples that may benefit from re‐purification. Overall, the assay is an excellent method to quantify mtDNA and is useful for determining the best analytical approach for successful sequencing.     

Orientalism,otherness, and the Soviet empire: travelogues by Latvian writers of the Soviet period

ABSTRACT

The development of travel writing in a national literature is closely related to the geographical and mental cartography that characterizes that territory. This article investigates Latvian travelogues of the Soviet era as encounters with other nations within the Soviet empire in order to analyze the role of Latvian travel writings in shaping a feeling of belonging to the empire. In addition to fostering obedience and a sense of belonging to the Soviet empire during the first 15 oppressive years of the Soviet regime, Latvian travelogues of the later years represented the Soviet empire as a conflictual site of the destruction of traditional values and landscapes.     

Imagining the modern city: Miyamoto [Chūjō] Yuriko in Moscow and London, 1927–1930

After her life-changing sojourn in the Soviet Union and Europe in the late 1920s, Miyamoto [Chūjō] Yuriko (1899–1951) devoted herself to the cause of communism. Her ideological conversion was rooted in the context of the modern city – in particular, Moscow and London – and the close interrelation between literary and filmic modernism and the modern city is evident in two key articles she wrote during her time abroad. In this article I will analyse how Yuriko represented her profoundly political experience and interpretation of two cities embodying alternative, opposed modernities – communism and capitalism – through modernist literary techniques and the new artistic technology of film.     

Identification and Individualization of Lophophora using DNA Analysis of the trnL/trnF Region and rbcL Gene

Lophophora williamsii (peyote) is a small, spineless, greenish‐blue cactus found in Mexico and the southwestern United States. Ingestion of the cactus can result in hallucinations due to its content of mescaline. In the United States, L. williamsii is classified as a Schedule I controlled substance. In this study, we use DNA analysis of the chloroplast trnL/trnF region and chloroplast rbcL gene to identify the individuals of Lophophora. Using the rbcL gene, Lophophora specimens could be distinguished from outgroups, but species within the genus could not be distinguished. The trnL/trnF region split the Lophophora genus into several groups based on the length and substructure of an AT‐rich segment of the sequence. Our results indicate that the genetic variability at the trnL/trnF locus is greater than previously recognized. Although DNA structures at the trnL/trnF region and rbcL gene do not align with the classification of Lophophora species, they can be used to aid in forensic analysis.     

耗散结构论对英语写作的启示

英语写作是一个复杂、动态的思维过程。事实上,它是一个耗散结构。英语写作是一个开放的系统,远离平衡态系统,非线性系统和一个有序的系统。研究耗散结构理论可以为英语写作教学提供一个新的研究视点,以期改善写作教学,全面提高学生的英语写作水平。     

日据时期旅行文学论述：身份认同与现代性

日据时期旅行文学研究在台湾方兴未艾。台湾文论界通过对旅行日本、欧美、中国大陆的不同游记的论述,探讨日据时期台湾人身份认同的暧昧两难。台湾社会特殊的历史际遇造成其现代性具有多种不同的表现形态。     

The Literary Journalism of Guadalupe Loaeza and Cristina Pacheco

Abstract — This article examines the writings of the Mexican literary journalists, Guadalupe Loaeza and Cristina Pacheco. It traces the political agendas of the writers through an analysis of their work in two collected volumes, Las niñas bien (Loaeza) and Sopita de fideo (Pacheco). The paper argues that while each author focuses on opposite ends of the social scale, each piece of writing contains an implicit or explicit attack on the Mexican ruling classes which mismanage the economy, squander its wealth and condemn the majority to economic misery.     

《漱玉词》中的闺音原唱——兼及“男子而闺音”的比较分析

缠绵婉约的风格和女性化的柔婉抒情,是诗词两途的重要表现。＂男子而作闺音＂的婉约词,以女性口吻伤怀怨别,甚至能够以假乱真。但《漱玉词》立足于女性创作本位,抒写了天真烂漫的少女情怀、幽怨相思的少妇情怀、饱经忧患与淡忘世情的老妇情怀和意欲突破女性角色的性别焦虑这四种情感类型,其真实、全面、细腻是＂男子而闺音＂的代言词所不及的。这也是《漱玉词》对于词史的巨大贡献所在。     

Getting the fish to see the water: an investigation into students’ perceptions of learning writing skills in academic modules and in a final year real client legal clinic module

In 2010 Tonya Kowalski described the problems faced by students entering clinic for the first time as a one step backward, two step forward phenomenon. Students appeared initially unable to transfer skills and knowledge learned in earlier academic and other settings to clinic but once they were immersed in clinic their skills development improved rapidly. Clinic is often presented as a “bridge to practice” and delivered as the capstone to more traditional elements of an undergraduate degree. However, even with an integrated approach like that at Northumbria Law School, a seamless transition to the skills required for clinic is challenging and gives rise to a constant review of how best to prepare students. Our research focused on legal writing and used focus groups to find out how students participating in the year four clinic at Northumbria University perceived and adapted their previous experiences of writing for use in the clinical context. It identifies strategies which should be considered for integration into non-clinical modules and in the clinical module itself to facilitate this transition from academic orientated writing to practice orientated writing.