PowerPlex16 HS系统直接扩增法检测肋软骨

目的检验PowerPlex16HS直接扩增系统对软骨的有效性。方法采用PowerPlex16HS复合扩增系统对10例腐败尸体的肋软骨进行直接扩增检验。结果所检验肋软骨都获得了完整遗传图谱,检测成功率为100%。结论 PowerPlex16HS系统直接扩增法能够应用于肋软骨的DNA分型检验。     

PowerPlex21 系统在亲子鉴定中的应用评估

目的评估PowerPlex21系统20个STR基因座在亲子鉴定中的检验能力。方法用PowerPlex21系统检测1 704例亲子鉴定,评估该系统在亲子鉴定中的排除能力和突变率。结果采用PowerPlex21系统,累积非父排除率和累积个体识别力均大于0.999 999 999 999 999 999 999 999 999 999。1704例亲子鉴定中有265例排除亲子关系,最常见为8~10个基因座排除;44例表现为1个STR基因座突变。结论 PowerPlex21系统用于亲子鉴定是高效的。     

Expanding Microfinance in Brazil: Credit Utilisation and Performance of Small Firms

We take advantage of the natural experiment generated by the exogenous change in government policy towards microcredit to evaluate the impact of the increased supply of microcredit on the utilisation of credit by micro-entrepreneurs. Based on micro-entrepreneurs' survey and administrative data from a microcredit programme in Brazil, we show that: the increased supply of microcredit raised formal credit utilisation and this does not crowd out the use of informal credit sources; formal credit taking improves business performance; and returns are larger for women- than for men-owned firms, but males employ significantly more workers after taking formal credit than females.     

16～17世纪老挝世俗文学及其特点

16～17世纪是老挝世俗文学的蓬勃发展时期,出现了长篇叙事诗、寓言、诗体小说、纪事体散文等文学体裁,并涌现出大量的优秀文学作品。这些作品在内容和形式上与印度文学和佛教文学相互渗透融合,呈现出佛教化、民族化、同质化等特点。作品既充盈着浓厚的佛教色彩,又贯穿着老挝本土的伦理文化和道德文明。     

Genotype profile for fifteen tetranucleotide repeat loci in two Tibeto-Burman speaking tribal populations of Arunachal Pradesh, India

POPULATION: Two Tibeto-Burman-speaking Adi tribal populations of Arunachal Pradesh, India, Adi Pasi ( n =121) from Upper Siang district, and Adi Minyong ( n =33) from East Siang district were analyzed for polymorphisms at 15 microsatellite loci. The populations belong to Mongoloid ethnicity and are of special significance in genetic studies due to their small population size, relative isolation in remote hilly areas, and traditional subsistence patterns.     

Formpflichtige Abtretung gemeinschaftlicher Schadenersatz- und/oder Gewährleistungsansprüche im WE?

Die Abtretung von aus M?ngeln allgemeiner Teile der WE-Liegenschaft herrührenden Schadenersatz- und/oder Gew?hrleistungsansprüchen im Rahmen eines entgeltlichen "Generalvergleichs" (hier: zwischen den Parteien eines Generalunternehmervertrags) an die Eigentümergemeinschaft/den einzelnen Wohnungseigentümer ist grunds?tzlich formfrei. Dem Gesch?digten kann nur dann eine Verletzung seiner Schadensminderungspflicht (hier: "bislang unterlassene Sanierung" der M?ngel) angelastet werden, wenn entsprechende Ma?nahmen im Einzelfall nach Treu und Glauben erwartet werden durften.     

Präklusion bei (unbedingtem) Staffelmietzins

Bei Vereinbarung eines für unterschiedliche Perioden von vornherein fest vereinbarten Mietzinses unterschiedlicher H?he ("unbedingter Staffelmietzins") beginnt die dreij?hrige Pr?klusivfrist des § 16 Abs 8 MRG auch hinsichtlich aller sp?ter wirksam werdenden Mietzinse mit dem Zeitpunkt der Mietzinsvereinbarung zu laufen.     

Allele frequency distribution based on 17 STR markers in three major Dravidian linguistic populations of Andhra Pradesh, India

Allele frequencies for 15 tetranucleotides and 2 pentanucleotides repeat loci were determined in 317 unrelated, healthy individuals of Andhra Pradesh, India, belonging to three pre-dominant endogamous populations namely, Kappu Naidu, Kamma Chaudhary and Kapu Reddy. Adherence to the expectations of the Hardy-Weinberg equilibrium (HWE) was confirmed for all loci with few exceptions, which were not significant after applying Bonferroni's correction. Statistical parameters of forensic interest; observed heterozygosity, probability of homozygosity, probability of extact test, power of discrimination, match probability, polymorphism information content, power of exclusion and mean paternity index were determined for all loci. The present study reveals that Penta E and D2S1338 are the most informative loci in all the studied populations. The combined power of discrimination was greater than 0.976, whereas the cumulative power of exclusion gave an expected value of 0.9999 for all the tested microsatellite loci. No difference was observed in the discriminatory power of 15 loci in studied populations on comparison with other populations of India. Population differentiation tests revealed significant differences between the studied and neighboring populations at several loci. Analyzed parameters indicate the utility and efficacy of the studied 17 STR systems as a powerful tool in forensic human identification, paternity testing and human population genetic studies.     

Effect of blood stained soils and time period on DNA and allele drop out using Promega 16 Powerplex kit

Blood stained soils may be of great interest in forensic incidents. Amplification of DNA from soil is often inhibited by co-purified contaminants. Different soils types from Pakistan and Turkey were stained with blood and samples were collected systematically after specified intervals. Rapid, inexpensive, large-scale DNA extraction method involving minimal purification was developed. DNA was quantized using Spectrophotometer and Fluorometer and was confirmed by Agarose Gel Electrophoresis. DNA extracted from different soils in different periods showed a remarkable decrease in yield as well as degradation in every extraction. PCR amplification was performed using various DNA targets present in Promega 16 Powerplex^® System kit. Amplification could not carry out in all loci especially in degraded samples taken after 20 days. Allele n locus drop out was noticed which shows that DNA was degraded. For some loci more than 2 alleles were also noticed showing contamination while working with the blood stained soils.     

15 STR loci frequencies in the population from Santa Catarina, Southern Brazil

Allele frequencies for 15 short tandem repeats (STR) loci were determined with a sample of 3000 unrelated individuals from the population of Santa Catarina, Southern Brazil. The loci are most commonly used in forensic and paternity testing, being analyzed by the Powerplex 16 (Promega) commercial kit. The data shows that most polymorphic loci were Penta E and FGA. The distributions of the genotypes in the evaluated loci are in Hardy–Weinberg equilibrium. Comparative analyses between our population data and other Brazilian populations are presented. The calculated forensic parameters showed that the loci are useful for the solution of forensic problems in Brazilian Southern region.