牛分枝杆菌重组MPT83蛋白间接ELISA方法的建立

以纯化的牛分枝杆菌重组MPT83蛋白为包被抗原,建立了检测牛分枝杆菌抗体的间接ELISA方法。确定了间接ELISA各组分的最适反应条件:抗原包被浓度为1μg/mL,酶标二抗稀释度为1∶1600,血清稀释度为1∶60,抗原和血清、血清和二抗均在37℃反应30 min,底物在37℃显色15 min,D655 nm阴性、阳性临界值为0.5。经阻断试验、交叉试验、重复性试验,表明该方法特异性强、重复性好。用该方法对18份结核菌素试验阳性牛血清和36份结核菌素试验阴性牛血清进行检测,结果显示,阳性血清的符合率为27.8%,阴性血清的符合率为91.7%。     

副猪嗜血杆菌抗体间接血凝检测方法的建立及应用

将副猪嗜血杆菌(Haemophilus parasuis,HPS)血清型4、5型分离菌株经超声波破碎处理后的产物致敏醛化红细胞,建立了检测HPS抗体的间接血凝试验方法。最适反应条件为绵羊红细胞30℃醛化5 h,4、5型菌株浓缩抗原以1∶8稀释致敏红细胞。免疫猪2周后检出率达89%。对15种HPS血清型阳性血清进行检测,结果均为阳性;对猪瘟病毒、口蹄疫病毒、猪圆环病毒、猪细小病毒、猪伪狂犬病病毒、猪生殖与呼吸综合征病毒、猪肺炎支原体、猪链球菌、猪肺疫巴氏杆菌、Ⅰ相支气管败血波氏杆菌及胸膜肺炎放线杆菌阳性血清进行检测,结果均为阴性。敏感性为琼脂扩散试验的16倍。对1 665份猪血清进行检测,阳性率为47.1%。结果表明,该方法敏感性较高,特异性强,重复性好,可用于疫苗免疫后抗体水平的检测及副猪嗜血杆菌的流行病学调查。     

“直接适用的法“与公共秩序保留制度比较研究

在国际私法中,适用直接适用的法和公共秩序保留制度都可以达到排除外国法,从而维护本国的主权和利益的效果,其中"直接适用的法"作为20世纪中期兴起的一种理论学说,反映了随着国家职能的加强,国家要求进一步加强对政治、经济、生活干预的要求.而传统国际私法中的公共保留制度在近来也发生了变化.作为能够达到同样目的的两种手段,两者之间关系如何?是否具有互换性?本文拟就此问题进行分析阐述,并对公共秩序保留制度中间接限制模式的存在提出新的见解.     

全部为间接证据案件的审查

全部为间接证据的案件往往表现为言词证据缺失或者言词证据表现为间接证据的案件。全部为间接证据的案件,是一种较特殊的案件,因而其证据的审查判断必须遵守特殊的规则,其中包括间接证据的属实性、一致性、完整性、排他性和合理性。通过"链接法"、"排除法"、"反证法"的运用,得出唯一的、排他的正确结论。     

股东关系维度代理问题及其治理机制研究

股东“异质化”的现实演进和股东关系的弱式平等使得股东关系维度代理问题日渐成为公司治理的重点与难点。股东关系维度代理问题在中国股份公司中有其特殊性,如股东间的代理关系情境极其复杂、控制权私利攫取成本较低、中小股东消极主义尤为明显、多元维度代理问题相互交织等。公司立法必须注重强化自治和利益平衡,多措并举构建防范股东关系维度代理问题的结构性治理机制。     

汉代儒学的述行策略及其流变面向——兼论“罢黜百家”与百家“曲胜”

There are two paradox voices about the revival of Confucianism constructed by typical phenomena, the victory and the corruption, which display the two directions of Confucianism’s transformation in Han dynasty. Behind the paradox, it’s the academic trend of skills overweighing truth and inter-ccommodation between Zhuzi(诸子); tracing the history, the performative thinking of Confucianism accommodating Zhuzi and carrying out its kingcrafts by submitting to monarch can be clearly sketched. The Confucianism doesn’t win separately by banning Zhuzi , whereas it makes the others succeed indirectly.     

陕甘宁边区的民主选举原则小议

陕甘宁边区的政权依据新形式下的民主选举原则,既保证了无产阶级在各级政府中的地位,又广泛地吸收了各抗日阶级、阶层的代表到政权机关中去,从而体现了抗日民众当家作主的权利。这种民主选举原则在陕甘宁边区,以及其他抗日民主区域得到实施和发展,成为激发广大人民抗日积极性和创造性的动力,坚持和扩大民族革命战争无穷力量的源泉。今天,仍不失为我们应遵循的民主选举原则。     

关于中国反垄断立法的几点思考

中国反垄断立法进程缓慢源于思想认识、行政垄断、行业和政府的阻力。对垄断和不正＂-5竞争分别立法适合我国国情;设立多元化的反垄断执法机构有利于节省行政资源;如何设立地方反垄断执法机构对规制行政垄断起着重要作用。     

Displacing your Principal. Two Historical Case Studies of Some Interest for the Constitutional Future of Europe

The framework of public decisions, and particularly the provision of law, is seen here as an agency contract. What distinguishes this contract is the nature of the right delegated to the agent: The capacity to make law gives the opportunity to take advantage of the incompleteness of the constitutional contract. The agency relationship may be loosened or even reversed. This article tries to draw some lessons from the English and American history in that matter and applies them to the making of the future European State.     

Violence as a part of the drug scene

Our study explores the violence that occurs in the drug scene from the perspective of those who use illicit drugs in Finland. We conducted 56 theme interviews with people with experience using multiple substances and studied how they produced agency related to violent acts. We were interested in the kinds of meanings they gave to violence and how these meanings varied according to different positions: (1) experiencing violence (victim), (2) committing violence and (3) witnessing violence. We found that in the drug scene, these positions were not fixed but overlapping and intertwined. Violence was usually projected onto the psychopharmacological properties of the substances or rationalized as being necessary for survival. The relations to and positions of violence differed considerably between men and women. Whereas in men’s interviews violence was often anchored to masculine power and questions of honour, in women’s accounts it was often related to intimate partner violence. However, irrespective of the individual’s position and gender, violence appeared to be a damaging experience that weakened his or her self-esteem. This vulnerability is encountered when people wish to quit the drug scene and its internal normative rules.