兔F型多杀性巴氏杆菌的分离鉴定及其致病性

对从山东某獭兔养殖场病死仔兔肺中分离的1株疑为巴氏杆菌菌株进行了菌落形态学观察及培养特性、革兰氏染色、生化反应、仔兔致病性回归试验、小鼠毒力试验,并采用PCR方法对该分离株进行了种属及荚膜血清型鉴定。结果显示,该分离菌株革兰氏染色阴性,生化试验及PCR鉴定结果显示为F型多杀性巴氏杆菌,能引起獭兔仔兔肺出血、坏死,并致部分仔兔死亡。表明该分离菌株为比较少见的兔F型多杀性巴氏杆菌。     

我国民事诉讼标的及其识别问题分析

民事诉讼标的是指法院予以审理和裁判的对象。我国的民事诉讼标的理论吸收大陆法系诉讼标的理论基础,形成民事纠纷说、新二分支说、分别识别说与为实践部门普遍接纳的旧实体法说等诸学说。其争议焦点主要围绕给付之诉的诉讼标的识别标准进行。从利于诉讼实践的角度出发,仍应坚持实体法学说,但诉讼标的的识别要以原告向法院提出的实体法律关系或请求权为准,未向或未能向法院提出的实体法律关系或请求权不能作为识别依据。     

从龚如心巨额遗产继承案看我国文件鉴定工作的完善

应尽快制定全国统一的《文件检验鉴定程序规则》;组织制定全国统一的《文件检验鉴定技术标准》;进一步规范文件检验鉴定文书的制作;强化文件检验鉴定人出庭作证的意识,提高出庭作证的技巧;正确认识文件检验鉴定结论的作用,不能单以鉴定结论决定判决结果。     

论刑法对社会同一性的自我确认--适时性原则之讨论

法规范在认识上被充分确保与整个社会系统维持一种同一性关系的最关键点在于刑法对社会同一性予以自我确认,而这种自我确认的实现途径就在于将适时性作为刑事立法的一项基本原则。适时性原则,主要是指刑事立法要遵循适应社会现实需要,以确认与社会保持同一性的原则。其价值蕴涵反映出刑法对社会同一性自我确认的本质属性。     

猪瘟病毒GSLZ株的分离与鉴定

采集甘肃省兰州市某猪场疑似猪瘟样品,将RT-PCR检测为阳性的样品处理后接种PK-15细胞并盲传至第12代,结果在盲传2代之后的细胞培养物中均可检测到猪瘟病毒(CSFV)E2基因.经浓缩、纯化的病毒在电镜下呈约25 nm典型的CSFV病毒粒子,将其命名为GSLZ株.对E2基因核苷酸序列的分析显示,该分离株与标准参考毒株...     

两株乙型脑炎病毒的分离鉴定与生物学特性研究

2009年从四川多个地区的猪场采集流产死胎及库蚊样本12份,采用BHK-21细胞培养方法分离出2株乙型脑炎病毒,分别命名为JEV-CZ1与JEV-YA1,PrM/C基因与疫苗株SA-14-14-2的同源性分别为90.9%与99.6%,基因分型研究显示JEV-CZ1株为基因Ⅰ型乙型脑炎病毒,JEV-YA1株为基因Ⅲ型。分离株在BHK-21细胞中能产生明显的细胞病变,其细胞半数感染量(TCID50)分别为10-6.206 TCID50/0.025mL与10-4.943 TCID50/0.025mL。经脑内注射测得分离株对体重9～11g小鼠的半数致死量(LD50)分别为9.97LD50/0.1mL和6.95LD50/0.1mL,其中JEV-CZ1株毒力较强。小鼠接种分离株3～4d后出现抽搐、肢体痉挛、异常兴奋等神经症状,5～7d后全部死亡,剖检可见脑部充血、水肿;病理切片观察可见脑内神经细胞变性坏死,其他器官也出现不同程度的病变。结果表明这2个分离株符合乙型脑炎病毒的特征。     

决策行为中的后悔情绪

后悔与我们的日常决策、认知方式、情绪体验等都密切相关。当个体意识到或者想象出,先前采取其他的行为将产生更好的结果时,个体就会体验到后悔。许多研究证据表明后悔,将会影响人们的行为决策。具体介绍了后悔的影响因素以及对决策行为的作用。     

Mononationals,hyphenationals, and shadow-nationals: multiple citizenship as practice

Multiple citizenship has in recent decades moved from an unwanted phenomenon in international relations to a fairly common transnational status. Multiple citizenship has nevertheless so far been studied mainly as a political and juridical status by comparing national legislations. Much less notice has been given to actual dual citizens' citizen participation and construction of citizens' identities. Only when citizenship is studied as these kinds of practices do the hypothetic possibilities and problems associated with the status get their meanings and contents. This paper concentrates on examining dual citizens' identifications to their respective citizenships and how these affiliations transfer into possible citizen participation. Results are based on extensive analysis of survey (n = 335) and interviews (n = 48) carried out among dual citizens living in Finland. Contents and forms of dual citizens' national identification and citizen participation were reviewed through ideal types: resident-mononationals, expatriate-mononationals, hyphenationals, and shadow-nationals.     

mtDNA多态性在嗜尸性昆虫种类鉴定中的应用

嗜尸性昆虫的种类鉴定是利用昆虫学知识进行法医学研究的重要步骤之一.近年来研究表明,利用分子生物学技术,特别是线粒体DNA(mitochondrial DNA,mtDNA)序列分析技术对嗜尸性昆虫进行种类鉴别,能够得到较好的效果.本文对嗜尸性昆虫mtDNA的分子生物学特性、结构以及嗜尸性昆虫种类鉴定中常用的mtDNA序列...     

Positive identification by a skull with multiple epigenetic traits and abnormal structure of the neurocranium, viscerocranium, and the skeleton

Wormian bones are small ossicles appearing within the cranial sutures in more than 40% of skulls, most commonly at the lambdoid suture and pterion. During the skeletal analysis of an unidentified male war victim, we observed multiple wormian bones and a patent metopic suture. Additionally, the right elbow was deformed, probably as a consequence of an old trauma. The skull was analyzed by cranial measurements and computerized tomography, revealing the presence of cranial deformities including hyperbrachicrania, localized reduction in hemispheral widths, increased cranial capacity, and sclerosis of the viscerocranium. Besides unique anatomical features and their anthropological value, such skeletal abnormalities also have a forensic value as the evidence to support the final identification of the victim.