汗潜指印DNA提取方法的初步研究

目的建立渗透性载体及非渗透性载体上汗潜指印DNA的提取和检验方法。方法采用C-有-柱法及SiO2法两种DNA提取方法,PCR扩增后310型遗传分析仪检测。结果载玻片上3枚汗潜指印采用2种方法均可扩增出Amel及9个STR位点;纸上3枚汗潜指印用SiO2法检见Amel及9个STR位点,而用C-有-柱法检验结果不稳定。渗透性及非渗透性载体上1及2枚汗潜指印,采用上述两种DNA提取方法,检验结果均不稳定。结论所建立的方法可以检见渗透性及非渗透性载体上汗潜指印DNA,并达到同一认定的程度。     

和谐文化是民族地区和谐发展的潜质内核——以文化广西的建设为例

民族地区和谐发展是民族地区在新的历史阶段发展的美好前景和理想追求，文化在本质上是人的特质外化，文化和谐构成了民族地区和谐的支点显现。文章以广西为例，试从历史的进程、发展的视角、和谐的维度等观照文化和谐是民族地区和谐发展的潜质内核。并从文化和谐的视阈探讨民族地区社会和谐的新图景。     

DNA指纹数据库法律问题初探--美国的纷争和启示

分子生物学科技发展起来后,美国开始建立DNA指纹数据库,以提高辨别罪犯的效率和准确率.然而,这一做法却引起了广泛争议,主要围绕着强制采集DNA样本是否构成"不合理的搜查和扣押",利用DNA样本进行科学研究是否违反了"知情同意"的人体实验伦理原则,以及保存DNA样本是否会侵犯个人隐私这三个方面展开.这些纷争对我们的启示是:既要合理地利用科学技术,又要加强法制,保护公民的合法权利,防止科学技术的负面影响.     

关于指纹汗孔的初步研究

指纹作为法庭科学中非常重要的一种证据,它的价值已毋庸置疑。乳突纹线上的汗孔在现场纹线特别少的情况下,也可以进行人身识别。汗孔的间距、形态、数量以及单条乳突纹线上汗孔的排列等都是可以利用的特征。     

新型粉末状荧光502熏显手印荧光波段研究

目的运用新型粉末状荧光502可一步显现非渗透性光滑客体表面潜手印。为了选择最佳的激发光源和滤光镜来观察熏显后荧光效果,本文对该粉末的荧光性能进行研究。方法使用荧光光谱仪对新型粉末状荧光502进行激发光谱和发射光谱的测试,进而选择合适的激发光源及滤光镜来进行观察和拍照。结果新型粉末状荧光502的光谱范围较宽,在波长为235nm~580nm范围内均可进行激发,具有广谱性,其中对波长在530nm附近的激发光具有最大峰值,即荧光强度最大,对450nm和360nm附近的光也具有很高的吸收峰值,斯托克斯偏移量为45nm。宏观验证实验表明,在仪器分析所得的范围内,显现出的手印效果均较好。结论运用该粉末在熏显手印后可以在紫外、紫光、蓝光、黄光、绿光范围内进行激发,斯托克斯偏移大,荧光亮度高,在观察荧光效果时能够很好的消除背景荧光的干扰,也便于选择滤光镜。手印荧光效果除了受到熏显物质荧光性能的影响外,还与激发光源的品质、滤光片的透过性能以及检材固有的发光特点、客体表面性质有关。     

Surface Modification for the Collection and Identification of Fingerprints and Colorimetric Detection of Urea Nitrate

Glass surfaces were modified with a combination of dyes and reagents to allow for the potential simultaneous recording of a detailed fingerprint and the detection of the explosive urea nitrate (UN), as a proof of principle of surface modification for simultaneous linking of identity to manipulation of explosives. By coating microscope slides with 9,10‐diphenylanthracene (DPA), p‐dimethylaminobenzaldehyde (p‐DMAB) and p‐dimethylaminocinnamaldehyde (p‐DMAC), a colorimetric change was observed in the presence of UN, while revealing a fingerprint with enough resolution to isolate at least 10 minutiae. This is the first step in creating point‐of‐care devices capable of detecting low concentrations of explosives and drug metabolites and connecting them to a fingerprint.     

A Novel Method for the Photographic Recovery of Fingermark Impressions from Ammunition Cases Using Digital Imaging

The photographic preservation of fingermark impression evidence found on ammunition cases remains problematic due to the cylindrical shape of the deposition substrate preventing complete capture of the impression in a single image. A novel method was developed for the photographic recovery of fingermarks from curved surfaces using digital imaging. The process involves the digital construction of a complete impression image made from several different images captured from multiple camera perspectives. Fingermark impressions deposited onto 9‐mm and 0.22‐caliber brass cartridge cases and a plastic 12‐gauge shotgun shell were tested using various image parameters, including digital stitching method, number of images per 360° rotation of shell, image cropping, and overlap. The results suggest that this method may be successfully used to recover fingermark impression evidence from the surfaces of ammunition cases or other similar cylindrical surfaces.     

论潜伏性人身损害的赔偿

随着科学技术的发展和疾病知识的普及,潜伏性人身损害的赔偿问题日益引起重视。既有立法基本不对损害进行界定,潜伏性人身损害更不会在立法文本中得到体现,但是既有损害概念的学理解释可以为潜伏性人身损害的赔偿提供认识基础。对潜伏性人身损害予以赔偿有其必要性,但是也会对传统侵权法带来挑战。虽然如此,既有司法实践还是积极回应受害人的赔偿诉求,从概念扩张和利益平衡等角度进行了大胆探索。为了有效解决潜伏性人身损害的赔偿问题,既需要从损害概念的更新方面做出努力,又需要从诉讼中的证明责任、证明能力等角度进行变革。     

Fingerprint reagents with dual action: color and fluorescence

We define "dual fingerprint reagents" as chemical formulations that produce with latent fingerprints in one stage impressions that are both colored and fluorescent. Solutions containing ninhydrin and group IIb metal salts appear to be true dual reagents. Application of these formulations to latent fingerprints on paper is as efficient as the two-step process beginning with ninhydrin and followed by treatment with metal salt. In the color mode, fingerprint detectability with the two ninhydrin-metal salt reagents (one with zinc chloride and the other with cadmium chloride) is comparable with that of ninhydrin itself, in spite of the difference in color. The sensitivity is significantly higher in the fluorescence mode. To view the latent impressions the exhibits are treated with ninhydrin-metal salt reagents and observed under white light illumination and under fluorescence conditions. Cooling to liquid nitrogen temperature enhances the fluorescence considerably. In the shorter wavelength domain, ninhydrin-metal salt reagents exhibit higher sensitivity than the recently reported dual reagent, genipin. The latter is advantageous, however, in the longer wavelength domain, on paper items with strong self-fluorescence, such as brown wrapping paper or paper printed with fluorescent ink. Upon reduction of the ninhydrin concentration 10-fold, ninhydrin-metal salt formulations become purely fluorogenic reagents; no color is noticed but the fluorescence is as intense as with concentrated solutions. Working at lower concentrations is an advantage from ecological and economical viewpoints.     

Infrared spectroscopic imaging for noninvasive detection of latent fingerprints

The capability of Fourier transform infrared (FTIR) spectroscopic imaging to provide detailed images of unprocessed latent fingerprints while also preserving important trace evidence is demonstrated. Unprocessed fingerprints were developed on various porous and nonporous substrates. Data-processing methods used to extract the latent fingerprint ridge pattern from the background material included basic infrared spectroscopic band intensities, addition and subtraction of band intensity measurements, principal components analysis (PCA) and calculation of second derivative band intensities, as well as combinations of these various techniques. Additionally, trace evidence within the fingerprints was recovered and identified.