口蹄疫病毒3C基因与增强型绿色荧光蛋白基因的真核共表达

为验证siRNAs对口蹄疫病毒(FMDV)复制的抑制效果,用反转录聚合酶链反应(RT-PCR)扩增Asia 1型口蹄疫病毒Jiangsu/China/2005株的3C基因,克隆入增强型绿色荧光蛋白(EGFP)表达载体pEGFP-N1中,并进行双酶切、PCR及测序鉴定。将阳性重组质粒转染PK-15细胞,检测EGFP的表达和3C基因转录水平。结果显示,经PCR及双酶切鉴定,目的基因片段大小与预期相符,测序结果与Jiangsu/China/2005株相应序列一致。荧光显微镜和流式细胞仪均检测到细胞内有EGFP表达,实时荧光定量PCR检测到细胞内有3C基因的转录。证实,成功构建了FMDV 3C基因与EGFP共表达质粒并在PK-15细胞中获得了表达。     

On the promise and perils of citizenship: heuristic concepts,Zimbabwean example

Increasingly, struggles in the name of citizenship inspire and catch the imagination and support of individuals and groups found in a variety of locales within a nation as well as transnational spaces. At the same time, their consequences may be quite different from the assumptions and dreams of those involved in perpetuating and imagining these struggles. To analyse how new social citizenship claims can embolden and channel struggles in particular directions with varied results – the promise and perils of citizenship more broadly – I suggest that one should pay attention to the promulgators of such visions of citizenship, the techniques of promoting their claims and the cultural politics and political economies of belonging in the locales of mobilization. Drawing on an ethnographic example of a farm labour struggle in the late 1990s in Zimbabwe, I explore the importance of attending to wider shifts in the political importance of citizenship as well as its entanglement in particular localities. Through examining how farm workers are situated through such struggles, I show the promise and limits of citizenship in addressing social justice concerns of a group historically marginalized through racialized, classed and gendered processes.     

‘The sleep has been rubbed from their eyes’: social citizenship and the reproduction of local hierarchies in rural Hungary and Romania

In this paper, we make three interrelated points. First, while much of the recent literature on new forms of citizenship has focused on the diversity of large cities and new forms of migration, we seek to establish rural sites as important arenas for negotiating citizenship. We stress that far from being homogeneous, villages in their struggles over belonging are affected by long-standing diversity as well as global discourses. Second, we seek to complicate the interpretation of the demise of socialism as a radical break manifested in a diminished role of the state. We show that if the central state retreats, local state actors may gain in importance for local negotiations of citizenship. Third, we explore how the local state actors sometimes use their new powers over social rights to recreate boundaries of belonging through public performances tied to the administration of these rights. We go on to explore the normative basis for these performances and indicate that membership is still based on a contribution of work to the common good. This can best be conceptualised as a shifting continuity rather than a sharp break after 1989.     

‘Changing teams’: a participant perspective on citizenship ceremonies

Several European countries of immigration have recently introduced citizenship ceremonies on either a mandatory or voluntary basis. Yet, little is known about how the target group for such ceremonies experiences them, and to what extent citizenship ceremonies achieve their goals of contributing to a greater sense of belonging. In this article, we analyse interviews with more than 50 newly naturalised Norwegian citizens about their reasons to attend or to not attend the voluntary ceremony in Norway. We find little resistance to the idea of citizenship ceremonies. For some participants, the ceremony seems to have great emotional value; the majority, however, chooses not to participate.     

Dual citizenship and theories of democracy

Dual/multiple citizenship has become a widespread phenomenon in many parts of the world. This acceptance or tolerance of overlapping memberships in political communities represents an important element in the ongoing readjustment of the relationship between citizens and political communities in democratic systems. This article has two goals and parts. First, it evaluates dual citizenship from the perspective of five normative theories of democracy. Liberal and republican as well as multicultural and deliberative understandings of democracy deliver a broad spectrum of arguments in favour of dual citizenship. Only communitarians fear that dual citizenship endangers national democracies. Nevertheless, empirical evidence and national policies largely contradict these fears. The second part of the article reverses the perspective and shows that most theories of democracy do not only legitimate and facilitate the acceptance of dual citizenship – the phenomenon of multiple citizenships induces innovation in democratic theory in turn. A second look at the relationship between dual citizenship and theories of democracy reveals that dual citizenship stimulates refinements, expansions and reconceptualisations of these theories for a transnationalising world.     

Citizenship values and asset accumulation: the case of Argentine migrants

Using diverse conceptualisations of citizenship, this article analyses the effect of the accumulation of civic and political assets on the transformation of citizenship values among Argentine migrants to Spain and returnees. Focusing on the transnational spaces, this article analyses important civic and political capabilities accumulated by a group of migrants and explores the impact of the assets accumulated in the transnational context. This research uses data drawn from 19 Argentine immigrants to Barcelona and 30 Argentine returnees from the cities of Madrid, Barcelona and Palma de Mallorca. Findings indicate that the migratory experience generated gains in the civic and political capabilities of this group of migrants and returnees and that living in Spain promoted the development of a more responsible, analytical and, in some cases, active citizenship. As holders of ‘multiple-perspectives’, interviewees were in a privileged position to critically analyse both the sending and receiving societies. Moreover, respondents implemented a number of practices acquired in the host society, in their home society, although this transfer generally remained at an individual level.     

Reconfiguring citizenship and nationality: dual citizenship of Taiwanese migrants in China

Many researchers have redefined citizenship to better understand the membership status aspired and demanded by contemporary migrants. As a result, the concept of ‘membership’ as opposed to citizenship was proposed in delineating the decoupling between citizenship and nationality; immigrant demands for rights and state policies in response can thereby be interpreted without considering the political meanings of citizenship. However, the decoupling of citizenship and national identity can be challenged when it comes to dual citizenship, especially when the homeland and host states are engaged in political tensions. This article examines the shifting policies of China (the People's Republic of China, or PRC) and Taiwan (the Republic of China, or ROC) towards the citizenship conferred to Taiwanese migrants in China. The findings of this research suggest that political dimension (including political rights and obligations) should be regarded as an integral part of citizenship (i.e. national membership) especially in the rival-state context. The Taiwan–China case can contribute to our understanding of citizenship policy changes under the double pressure of inter-state rivalry and globalization. The globalizing forces help create conditions for ‘flexible citizenship’ in the ‘zones of hypergrowth’, while in the case of Taiwan–China inter-state competition draws governments and people back to zones of loyalty, the nationally defined memberships.     

Introduction: claiming social citizenship

Social citizenship in the classical sense of T.H. Marshall has been declared to be eroded and to have lost its significance. The introduction to this special issue challenges this assumption and argues that recent anthropological work on social citizenship in post-colonial, post Cold War and post-socialist states have shown that social citizenship is relevant and is being claimed by citizens of these states. Historical notions of citizenship as well as claiming rights to state support in return for having worked for the state are at work here. Furthermore the contributions to this issue illustrate how notions and practices of social citizenship compete and sometimes replace other practices of claiming citizenship on the basis of ethnicity, nationality or cultural ties.     

Labor migration,ethnic kinship,and the conundrum of citizenship in Turkey

This paper explores the ambiguous purchase that claiming Turkish ethnicity has in Bulgarian Turkish migrants' attempts to access formal and social citizenship. I suggest that despite the new Citizenship Law, which appears to eliminate ethnic privilege, the emphasis on Turkish ethnicity continues to play a significant role in the migrants' attempts at inclusion. I seek to resolve this seeming tension between, on the one hand, the continuing significance of ‘Turkishness’ in migrants' discursive claims, and, on the other hand, the failure of most of these claims to materialize in practice by addressing the question of social and economic capital. Although ethnic belonging continues to be an important facet of citizenship, social class makes a significant difference in determining who qualifies as a citizen and has access to social citizenship. I thus argue that we need to expand the current terms of the debate on the inclusiveness of citizenship in Turkey, which revolve around ‘denationalization’ and ‘postnationalism,’ to include questions of class-based exclusion.     

Historical apologies as acts of symbolic inclusion – and exclusion? Reflections on institutional apologies as politics of cultural citizenship

Institutional apologies for historical injustices can be conceived as acts of symbolic inclusion directed to people whose collective experiences and memories of the past have not been recognized in the hegemonic narratives of the past. However, in this article it is argued that such apologies also have exclusionary potential as vehicles of symbolic politics of citizenship in that they may designate the apologizing community, so that it effectively excludes cultural ‘aliens’, like migrants, from the community of ‘remedial’ citizens. The article suggests a crucial point is the rhetoric shifts when one is appealing to both cultural and political solidarity, as when apologizing in the name of the state but simultaneously invoking ‘our’ nation and ‘our’ history. Thus, the increasing number of institutional historical apologies is not necessarily incompatible with the trend of reinforcing the symbolic boundaries around ‘our’ historical–cultural communities that has been visible recently, e.g. in the demands for cultural canons and citizenship tests in many Western societies.