Quantification of human mitochondrial DNA using synthesized DNA standards

Successful mitochondrial DNA (mtDNA) forensic analysis depends on sufficient quantity and quality of mtDNA. A real-time quantitative PCR assay was developed to assess such characteristics in a DNA sample, which utilizes a duplex, synthetic DNA to ensure optimal quality assurance and quality control. The assay's 105-base pair target sequence facilitates amplification of degraded DNA and is minimally homologous to nonhuman mtDNA. The primers and probe hybridize to a region that has relatively few sequence polymorphisms. The assay can also identify the presence of PCR inhibitors and thus indicate the need for sample repurification. The results show that the assay provides information down to 10 copies and provides a dynamic range spanning seven orders of magnitude. Additional experiments demonstrated that as few as 300 mtDNA copies resulted in successful hypervariable region amplification, information that permits sample conservation and optimized downstream PCR testing. The assay described is rapid, reliable, and robust.     

Effectiveness of wound cleansing treatments on maggot (Diptera, Calliphoridae) mortality

Myiasis is defined as an infestation of the organs and/or tissues of human and other animals by fly maggots. Fly species that normally breed in meat or carrion (Diptera: Calliphoridae, Sarcophagidae) may become involved in cutaneous myiasis by colonizing preexisting wounds. Reports of human wound myiasis contracted in hospitals and nursing homes, especially when patients are chronically ill or bed-ridden, are not uncommon across North America and often result in cases of neglect and civil litigation. Based on a case history dealing with this latter situation and circumstances surrounding the treatment of maggot infestation, we designed an experiment to assess the effectiveness of wound cleansing solutions on maggot mortality. Treatments, consisting of four commonly used cleaning solutions (isopropyl alcohol, Dakin's solution, iodine, and hydrogen peroxide) and a control (deionized water), were applied to experimental units (n=5), with each unit consisting of groups of actively feeding Lucilia sericata maggots (Diptera: Calliphoridae). Every 24h, treatments were applied and mortality was assessed for the duration of the study (14 days). Total mean mortality increased over the duration of the experiment, with an initial large increase (10-25%) after the first treatment application, followed by a gradual increase over the remainder of the study. General differences among treatments indicated greatest mean total mortality for Dakin's solution (sodium hypochlorite) (46%), followed by isopropyl alcohol (42%), Betadine (37%), hydrogen peroxide (33%) and lowest mortality for the control (25%); however, no statistically significant differences were observed among treatments and no treatment resulted in 100% maggot mortality. Traditional wound cleansing solutions may not be sufficient for maggot infestations of pre-existing wounds and supplemental treatments may be necessary to effectively treat cases of wound myiasis.     

Social Equity and COVID-19: The Case of African Americans

Emerging statistics demonstrate that COVID-19 disproportionately affects African Americans. The effects of COVID-19 for this population are inextricably linked to areas of systemic oppression and disenfranchisement, which are exacerbated by COVID-19: (1) health care inequality; (2) segregation, overall health, and food insecurity; (3) underrepresentation in government and the medical profession; and (4) inequalities in participatory democracy and public engagement. Following a discussion of these issues, this essay shares early and preliminary lessons and strategies on how public administration scholars and practitioners can lead in crafting equitable responses to this global pandemic to uplift the African American community.     

A semiautomated radioimmunoassay for mass screening of drugs of abuse.

A rapid, semiautomated radioimmunoassay system for detection of morphine, barbiturates, and amphetamines is described. The assays are applicable to large drug abuse screening programs. The heart of the system is the automatic pipetting station which can accomplish 600 pipetting operations per hour. The method uses 15 to 30 mul or urine for the morphine and barbiturate assay and 100 mul for the amphetamine and combined morphine/barbiturate assays. A number of other drugs were tested for interference with the assays and the results are discussed.     

Formalization and consistency heighten organizational rule following: Experimental and survey evidence

This study examines the attributes of organizational rules that influence rule following. Rule following fosters organizational effectiveness by aligning individual behaviours with organizational preference. While a range of theoretical explanations have been offered for rule following, the characteristics of rule design and implementation have received less empirical attention. Borrowing from the green tape theory of effective rules, this study examines the influence of two particular characteristics—rule formalization and rule consistency—on rule following. Three studies, which include two vignette experiments and a survey of two local government organizations, provide the data for the research. The results suggest that rule formalization and rule consistency independently increase rule following, with mixed evidence of interaction effects. The broad implication is that public managers must attend to both rule design and implementation to foster organizational rule following.     

Transforming international communications strategies

The strategy used by governments to communicate with foreign populations has changed dramatically in the twentieth century. The need perceived by governments to use such a strategy in the first place derived from numerous social changes in the 150 years before World War I, most particularly the increased role played in politics by the masses organized in nation‐states. The shifting strategy of persuasive communication since then forms part of a broader transformation of international political communication which includes technological change, organizational developments, and absolute but not necessarily relative growth in international communications transactions.

The predominant strategy developed in World War I was propaganda. It used fairly straightforward appeals to rationality, sense of morality, and such important but readily accessible emotions as hatred. Its excesses, especially the wide dissemination of atrocity stories which did not bear up well under later dispassionate examination, at once made all propaganda suspect and alerted governments to the necessity of refining their communicative techniques for the eventuality of future international conflicts.

The years before and during World War II saw international communicators—especially Goebbels in Nazi Germany and the Anglo‐American psychological warriors—develop a new strategy which utilized new knowledge about the psychological roots of human behavior. In addition to straightforward appeals to the consciousness of individuals, they sought to manipulate their audiences by playing on their deep‐seated desires and fears. Of particular importance for Nazi Germany were the allegedly racial ties of the individual to a people and the need for figures of authority.

First major experiments were made in World War II to develop an even more basic strategy of international communication: structuring the situation in which people learn their predispositions, perspectives, and behaviors. Its basic idea is to create situations in which the communicator does not have to tell the targeted audience anything at all, but in which the audience, left to its own devices, can only come to the conclusions desired by the communicator. Thus strategic bombing sought to destroy the morale of the enemy's home front and, through that action, to weaken its war‐making capacity. The postwar occupation of Germany originally sought to create a new environment in which Germans could be reeducated to democracy. Subsequent emphasis has also been on communications aimed at generating certain predispositions, such as a desire for consumer goods or a particular political style, which then spawn appropriate perspectives and behaviors.

This new strategy of structural communication has its limits. The most important of these is the difficulty of controlling all aspects of a foreign population's communications environment (which includes traditions, face‐to‐face networks, and conflicting sources of information). The potential importance of the strategy nonetheless makes it likely that governments will continue to work toward its development and implementation for their own international political purposes.     

A Test of Hartnett's Revisions to the Pubic Symphysis and Fourth Rib Methods on a Modern Sample

Estimating age at death is one of the most important aspects of creating a biological profile. Most adult age estimation methods were developed on North American skeletal collections from the early to mid‐20th century, and their applicability to modern populations has been questioned. In 2010, Hartnett used a modern skeletal collection from the Maricopia County Forensic Science Centre to revise the Suchey‐Brooks pubic symphysis method and the ??can et al. fourth rib methods. The current study tests Hartnett's revised methods as well as the original Suchey‐Brooks and ??can et al. methods on a modern sample from the William Bass Skeletal Collection (N = 313, mean age = 58.5, range 19–92). Results show that the Suchey‐Brooks and ??can et al. methods assign individuals to the correct phase 70.8% and 57.5% of the time compared with Hartnett's revised methods at 58.1% and 29.7%, respectively, with correctness scores based on one standard deviation of the mean rather than the entire age range. Accuracy and bias scores are significantly improved for Hartnett's revised pubic symphysis method and marginally better for Hartnett's revised fourth rib method, suggesting that the revised mean ages at death of Hartnett's phases better reflect this modern population. Overall, both Hartnett's revised methods are reliable age estimation methods. For the pubic symphysis, there are significant improvements in accuracy and bias scores, especially for older individuals; however, for the fourth rib, the results are comparable to the original ??can et al. methods, with some improvement for older individuals.