Alpha-amylase kinetic test in bodily single and mixed stains

Recently, in Italy, a murder and a putative sexual violence was accomplished on a child. A bodily fluids mixture on the child's underwear between the victim (female) and the suspect (male) was ascertained by short tandem repeat (STR) DNA typing and, due to the absence of seminal fluid, saliva from the suspect and urine from the child was hypothesized. In order to investigate the possibility of specifically and rapidly detecting saliva stains both alone and mixed with other bodily fluids, we used a quantitative spectrophotometric technique, named Amylase test, for the detection of alpha-amylases. We determined alpha-amylase activity and reaction kinetic curves in several samples collected from the child's underwear. In order to confirm our intuition, we first tested saliva, perspiration, and urine, singularly and in mixtures; second, several forensic stains including saliva, perspiration, urine stains, saliva/perspiration, and saliva/urine mixture stains were tested. Evaluating alpha-amylase activity values and time-course curves' behavior of alpha-amylase reactions we were able to recognize successfully, in all cases, the presence of saliva and to distinguish it specifically from other bodily fluids containing alpha-amylase. A further confirmation of our result was provided by STR DNA typing on several areas of the underwear: a clear correlation between alpha-amylases activity and male DNA was detected on all the samples evaluated.     

In Search of Coherence in EU Foreign Policy

While attributing the main tasks relating to CFSP to various institutions, the EU Treaty mirrors the traditional EU structure, which does not appear to be able to provide the coherence and efficiency needed in the foreign policy field. The Constitutional Treaty attempted to achieve coherence by introducing important changes, including an EU Minister for Foreign Affairs (the “double-hatting” solution). After the CT ratification failures, however, thought must be directed at finding steps that lead towards the CT solutions but are at the same time compatible with the TEU. While double-hatting is difficult to reconcile with some of the TEU's provisions, other measures and devices could to some extent anticipate the CT's perspective without contravening the treaties in force.