Analysis of Δ-tetrahydrocannabinol in oral fluid samples using solid-phase extraction and high-performance liquid chromatography–electrospray ionization mass spectrometry

An analytical method using solid-phase extraction (SPE) and high-performance liquid chromatography–mass spectrometry (LC–MS) has been developed and validated for the confirmation of Δ⁹-tetrahydrocannabinol (THC) in oral fluid samples. Oral fluid was extracted using Bond Elut LRC-Certify solid-phase extraction columns (10 cm³, 300 mg) and elution performed with n-hexane/ethyl acetate. Quantitation made use of the selected ion-recording mode (SIR) using the most abundant characteristic ion [THC + H⁺], m/z 315.31 and the fragment ion, m/z 193.13 for confirmation, and m/z 318.00 for the protonated internal standard, [d₃-THC + H⁺]. The method proved to be precise for THC, in terms of both intra-day and inter-day analyses, with coefficients of variation less than 10%, and the calculated extraction efficiencies for THC ranged from 76 to 83%. Calibration standards spiked with THC between 2 and 100 ng/mL showed a linear relationship (r² = 0.999). The method presented was applied to the oral fluid samples taken from the volunteers during the largest music event in Portugal, named Rock in Rio-Lisboa. Oral fluid was collected from 40 persons by expectoration and with Salivette^®. In 55% of the samples obtained by expectorating, THC was detected with concentration ranges from 1033 to 6552 ng/mL and in 45% of cases THC was detected at concentrations between 51 and 937 ng/mL. However, using Salivette^® collection, 26 of the 40 cases had an undetectable THC.     

Persistent Harassment: Targets and Perpetrators Among Young Adults

In Portugal, stalking has recently been recognized as a crime. This study used an online survey to ask 3,367 college students about their experiences of persistent harassment and targets’ and perpetrators’ perceptions of fear and conduct legitimacy. Targets (34.5%) typically mentioned that they had experienced a frightening experience but considered it to have been wrong but not very serious. Perpetrators (8.9%) mostly perceived that their targets had not experienced fear and judged their own conduct to have been normal. Ordinal logistic regressions indicated that intimate partner stalking, targets’ fear, and threatening and violent behaviors predicted less conduct legitimization for both targets and perpetrators.     

The Limits of a Commitment? Public Responses to Asylum Policy in Sweden over Time

It is hardly an exaggeration to claim that one of the most turbulent political areas in recent years has been asylum policy, which has disclosed a rapidly increasing inflow of asylum seekers, and, in many countries, has been followed by fierce media discussion and political controversies. In Sweden, this development has been heated as the Swedish self‐image is one of providing generous policies, which is also reflected in terms of strong refugee policy. The article uses this example to explore assumptions about public responsiveness in previous policy feedback literature and to examine the link between citizens' attitudes towards immigration and changes in asylum policy output, measured as asylums granted, over time in the period 1990–2015. It focuses especially on the link through which citizens become aware of policy output, operationalized as media visualization, and find that including media reveals a suppressed relationship between policy output and public attitudes. The relationship is negative and thus confirms the assumptions of the thermostatic models. Second, the article shows that feedback is mediated by political orientation: People defining themselves politically as right‐oriented respond with negative feedback when the number of granted asylums increases, while left‐oriented people do not change their attitudes. Based on these findings it is concluded, first, that analyses of democratic responsiveness need to incorporate a clear measure of the link by which exogenous factors become visible. Second, the importance needs to be stressed of considering important cleavages in the population in order to display responsiveness processes fairly.     

Lost in Santa Martha. Limitaciones para una etnografía en una cárcel de mujeres

En este artículo propongo un examen de algunos de los obstáculos establecidos por las autoridades penitenciarias para llevar a cabo un proyecto etnográfico en una cárcel de mujeres de la Ciudad de México. Mi intención es plantear la importancia de formas de investigación-acción en contextos que, como el mexicano, se ven afectados por una profunda crisis de los aparatos del Estado –en particular presto atención a los sistemas educativo, penal y de justicia- que impacta con mayor severidad a grupos vulnerables como es el caso de las mujeres en reclusión que están en el centro de este estudio de caso.     

Portuguese population and paternity investigation studies with a multiplex PCR--the AmpFlSTR Profiler Plus

A Portuguese Caucasian population of 146 unrelated individuals was studied. DNA samples were amplified by multiplex PCR for D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820 using the AmpFlSTR Profiler Plus PCR Amplification Kit (Perkin-Elmer). All loci met Hardy-Weinberg expectations. Forensic statistical parameters were according to those obtained by other authors. Statistical differences were observed concerning three loci when comparing the Portuguese Caucasian population and an Italian Caucasian population, although these differences mainly concern the less frequent alleles. Eighty-three paternity investigation cases were analysed. Exclusions in between three and nine loci were observed in all the 23 exclusion cases obtained. Most of the non-exclusion cases had probability of paternity > 99.9%. Two cases with an isolated genetic incompatibility between the alleged father and the child were detected, which may indicate probable mutation cases. These results demonstrate that the AmpFlSTR Profiler Plus is a suitable multiplex for paternity investigation in the Portuguese population.     

Allele frequencies data and statistic parameters for 16 STR loci-D19S433, D2S1338, CSF1PO, D16S539, D7S820, D21S11, D18S51, D13S317, D5S818, FGA, Penta E, TH01, vWA, D8S1179, TPOX, D3S1358-in the Rio de Janeiro population, Brazil

Allele frequencies for 16 short tandem repeats (STR) loci were determined with a sample of 230-300 unrelated individuals from the population of Rio de Janeiro, Brazil. The loci are the most commonly used in forensic and paternity testing, being analysed by the Identifiler (Applied Biosystems) and PowerPlex 2.1 (Promega) commercial kits. It was proved that Penta E and D18S51 are the most polymorphic loci.     

DNA fingerprinting of mosses

Our study introduces the use of DNA fingerprinting of clonal plants in combination with phylogenetic and vegetation studies as a prospective forensic tool in criminal investigations. In this homicide case, the bryophyte species found on the suspects were identified as Brachythecium albicans, Calliergonella lindbergii, and Ceratodon purpureus. Colonies of all three species occurred at the crime site. DNA fingerprinting analyses were conducted for B. albicans and C. lindbergii, which were expected to reproduce mainly clonally, unlike C. purpureus, and included samples found on the suspects and samples collected from the crime site and other locations. It was concluded that B. albicans found on the suspects was likely to originate from the crime scene and that the sample of C. lindbergii may also have originated from the same site.     

Juvenile Delinquency in Central European Cities: A Comparison of Registration and Processing Structures in the 1990s

The authors trace and compare the developments in recorded juvenile delinquency in Hamburg, Prague, Cracow and Budapest from 1991-1997 and then analyse the processing and selection procedures of the various justice systems. They devote special attention to ethnic minorities within this filtering process. The most salient feature is that the crime rates and processing structures in the former socialist countries display considerable similarities. It would almost be possible to speak of a specific type of criminal justice system with a typical form of reaction. While in the West, the large number of suspects is considerably reduced during later stages of selection to a much smaller number (those actually sentenced and/or imprisoned), what the authors call a funnel model, in the East a smaller number of suspected offenders enters this selection process, but tends to remain within it and be sentenced - the cylinder model. These procedural structures have changed little in the 1990s, and there has certainly not been any increasing alignment of the Eastern systems with the Western one. Indeed, the difference has, if anything, become greater. These lower crime statistics as compared with the West - represented here by Hamburg - are, however, not only the result of equally large discrepancies between the real crime rates, but in this regard the pro-active crime prevention measures of the police, which in Hamburg have caused the inclusion of an increased number of juveniles and foreigners in the crime statistics since 1995, have also had a great effect. The research project thus clearly demonstrates the importance of interpreting crime statistics neither as a true representation nor as a distorted reflection of the activities of a criminal justice system. Instead, these statistics should be seen as reflecting specific processing procedures and methods of crime control.     

Breastfeeding Duration and Reported Child Maltreatment in a Population-based Alaskan Birth Cohort

Journal of Family Violence - Research suggests that breastfeeding may reduce child maltreatment risk. Alaska has high rates of both breastfeeding initiation and maltreatment reports to Child...