Spectra interference between diquat and paraquat by second derivative spectrophotometry

A rapid and accurate method, combining solid-phase extraction and second-order derivative spectrophotomety approaches, is developed for the simultaneous determination of diquat (DQ) and paraquat (PQ) in blood, tissue and urine samples. Supernatant resulting from the precipitation of protein (with trichloroacetic acid) in plasma and tissue or Amberlite IRA-401 resin treated urine are passed through a mini-column packed with Wakogel gel (Silica gel). Analytes are then eluted with a non-organic solvent, 0.2mol/l HCl solution containing 2mol/l NH(4)Cl. UV spectrum of the eluent in 220-350nm range provides effective screen to detect the presence of DQ and/or PQ. In the presence of DQ or PQ alone, the analyte present is quantitated by conventional zero- or second-order derivative spectrophotometry. The calibration curve in the 0.1-5.0mg/l range for either analyte obeys Beer's law. When both DQ and PQ are present, their concentrations are determined by the peak amplitudes of their respective second-derivative spectra after the addition of alkaline dithionite reagent. Interference is negligible when the DQ/PQ concentration ratio is within the 5.0-0.2 range.Using a 2-ml of sample size, the detection limits for DQ and PQ in plasma are 0.02 and 0.005mg/l. The corresponding detection limits for urine samples (10ml sample size) are 0.004 and 0.001mg/l. Recoveries of DQ and PQ in triplicate plasma and urine samples spiked with 0.5mg/l of analytes are 93 and 85%. The precision of the proposed method resulting from triplicate study of spiked urine samples varies from 3.2 to 4.6% at 0.5mg/l of DQ and PQ, respectively.     

"传统"与"现代"的对话:论遗传资源获取及惠益分享与知识产权

信息社会中随着研究视角从"文化多样性"深入到"遗传多样性"领域,遗传资源荻取及惠益分享与知识产权的法律问题得到彰显.其根本冲突主要表现在三个层面:传统知识与原有知识产权保护体系的协调;遗传资源提供方与利用方的利益协调;经济利益与环境利益矛盾冲突的协调.就主体要件而言,群体主义权利主体模式具有可行性;就程序要件而言,事先知情同意规则、许可规则、来源地规则具有必要性;就知识产权体系下的惠益分享方式而言,积极参与具体研发过程具有战略性.在调整遗传资源与知识产权的诸多利益关系过程中,需要警惕四大风险:知识产权过度扩张而导致"反公有物悲剧";知识产权成为利益集团谋求私利工具并危害公共福利:利益分配新失衡致使"弱势主体"再次错失发展机遇;以牺牲环境利益为代价的技术开发.     

Determination of paraquat from formalin-fixed tissue

In this paper, a sulfuric acid digestion method and a clean-up technique by using cation exchange resin followed by XAD-2 resin has been developed for the determination of paraquat from formalin-fixed tissue at the submicrograms per gram level. Formalin-fixed tissue is dissolved by hot sulfuric acid, then paraquat is isolated and purified with cation exchange chromatography. The eluted paraquat forms an ion-pair with sodium dodecyl sulfate, it is then adsorbed on XAD-2 resin. Paraquat is eluted, extracted and reduced with solvent mixtures, NaCl solution and dithionite reagent, respectively. The calibration graphs of zero-order and second-derivative spectroscopy are linear in the range of 0.01-5.0 mg/kg. The relative standard deviation was less than 5% and the detection limit was 0.02 mg/kg based on 0.5-g samples. The sensitivity of the proposed method could be increased by using larger sample sizes. The method was precise and gave a quantitative recovery of paraquat spiked into formalin-fixed liver homogenates (78%). The proposed method has been satisfactorily applied to the determination of paraquat in the formalin-fixed tissues of suspected poisoned cases. It has been shown to be of great value in the field of forensic toxicology especially when formalin-fixed tissue only is available.     

The concentration of criminal victimization and patterns of routine activities

Although many repeat victimization studies have focused on describing the prevalence of the phenomenon, this study attempted to explain variations in the concentration of victimization by applying routine activities as a theoretical model. A multivariate analysis of repeat victimization based on the 2005 Taiwan criminal victimization data supported the general applicability of the routine activity model developed in Western culture for predicting repeat victimization. Findings that diverged from Western patterns included family income to assault, gender to robbery, and marital status, family income, and major activity to larceny incidents. These disparities illustrated the importance of considering the broader sociocultural context in the association between risk predictors and the concentration of criminal victimization. The contradictory results and nonsignificant variance also reflected untapped information on respondents' biological features and psychological tendencies. Future victimization research would do well to integrate measurements that are sensitive to salient sociocultural elements of the society being studied and individuals' biological and psychological traits.     

An empirical assessment of the process of restorative justice

This study involved an empirical assessment of restorative justice processes with an application of a theoretical model proposed by Presser and Van Voorhis (2002). Their model identified three common procedural activities associated with restorative justice: dialogue, relationship building, and communication of moral values. This study utilized secondary data, consisting of observation and interview data, originally obtained by Sherman, Braithwaite, Strang, and Barnes (1999) for their Reintegrative Shaming Experiments (RISE) in Australia, 1995-1999, to test the theoretical model. The results generally supported the hypothesis that the restorative justice program engaged offenders in dialogue, relationship building, and moral communication to a greater degree than traditional court proceedings. An unexpected result emerged in the interview data showing that violent offenders in the restorative justice program did not report a greater sense of relationship building than those in court proceedings. Possible explanations accounting for the anomaly are provided. Implications for policy and future studies derived from the findings are also discussed.     

论传统手工技艺的商业秘密保护

传统手工技艺是非物质文化遗产的重要组成部分。随着全球经济的发展,产业化、国际化进程给传统手工技艺带来了前所未有的冲击,因此,如何保护我国传统手工技艺已刻不容缓。本文从界定传统手工技艺的范畴入手,重点分析了当前我国传统手工技艺的发展现状,并在此基础上提出了适用商业秘密保护传统手工技艺的措施,特别是针对我国传统手工技艺适用商业秘密保护存在的困境,提出了解决对策。