Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X-12 QS Kit from blood samples on FTA cards

The onus of proof in criminal cases is beyond any reasonable doubt, and the issue on the lack of complete internal validation data can be manipulated when it comes to justifying the validity and reliability of the X-chromosomal short tandem repeats analysis for court representation. Therefore, this research evaluated the efficiency of the optimized 60% reduced volumes for polymerase chain reaction (PCR) amplification using the Qiagen Investigator® Argus X-12 QS Kit, as well as the capillary electrophoresis (CE) sample preparation for blood samples on Flinder's Technology Associates (FTA) cards. Good-quality DNA profile (3000–12,000 RFU) from the purified blood sample on FTA card (1.2 mm) were obtained using the optimized PCR (10.0 μL of PCR reaction volume and 21 cycles) and CE (9.0 μL Hi-Di™ Formamide and 0.3 μL DNA Size Standard 550 [BTO] and 27 s injection time) conditions. The analytical and stochastic thresholds were 100 and 200 RFU, respectively. Hence, the internal validation data supported the use of the optimized 60% reduced PCR amplification reaction volume of the Qiagen Investigator® Argus X-12 QS Kit as well as the CE sample preparation for producing reliable DNA profiles that comply with the quality assurance standards for forensic DNA testing laboratories, while optimizing the analytical cost.     

Embodying the Deepest Contradiction: A Rejoinder to Alan Rudy

Andrew Ross: The Chicago Gangster Theory of Life: Nature's Debt to Society . London: Verso. 1994.

Alexander Wilson: The Culture of Nature: North American Landscape from Disney to the Exxon Valdez . Cambridge, Mass.: Blackwell. 1992.

Simon Schama: Landscape and Memory . New York: Alfred A. Knopf. 1994.     

The Meta-industrial Class and Why We Need It

The paper suggests that the appropriate 'agents of history' in an era of globalisation and ecological crisis are 'meta-industrial' workers. This hitherto nameless class carries out hands-on reproductive labours at the interface of 'humanity' and 'nature' using 'holding skills', a grounded epistemology and ethic consonant with genuine democracy and local sustainability. Pointing to the unexamined neo-liberal assumptions of many environmental philosophers, the author suggests that only an 'embodied materialist' epistemology and ethic can do justice to class, race, gender, and species diversity.     

Moving to an embodied materialism

Edited by U.K. Green Party members David Kemball‐Cook, Mallen Baker, and Chris Haltingly and endorsed for publication by the Green Party Council, The Green Budget: An Emergency Programme for the United Kingdom was published by Green Print, London, in 1991. Joseph Schumpeter once wrote that the public budget is the “skeleton of the state stripped of all misleading ideologies.”.     

（Re）Integrating spirituality into higher education

This article examines the issue of accountability and governance among higher learning institutions in discharging their duties to inculcate good behaviour and mould their graduates into human business leaders. In order to appreciate the role of higher education and the requirement of the market place for graduates, we go backward in origin into the past to learn the education philosophy that has shaped today＇s vocation. The distinctiveness of this article is that we review the advances in the science of quantum physics to understand the current worldview of reality. The way forward for higher learning institution to reintegrate spirituality in molding young generation who actualize authentic leadership and rightness-of-action founded upon the interconnectedness model of the universe is deliberated.