人线粒体DNA序列分析在法医学中的应用研究及其进展

综述人线粒体DNA(m tDNA)序列分析在法医学种属鉴别、个体识别,以及个体年龄推断中的应用研究及其进展,展望对m tDNA异质性的研究及建立人m tDNA数据库,并具有重要的法医学实践意义。     

Sequencing of intersecting lines by combined lifting process and scanning electron microscopy

The chronological order in which two intersecting writing or typed strokes were made can be determined for several combinations of writing media by scanning electron microscope (SEM) examination, a lifting and transfer technique using Kromekote paper, or a combination of the transfer technique followed by SEM examination of the remaining material in the intersection. When non-destructive examination cannot provide unambiguous determination of sequence, judicious choice by the document examiner of which technique to use for a given combination of writing media and paper type can be made using known characteristics of the materials involved. The range of line-crossings which can be reliably sequenced is greater using a combined transfer and SEM technique than when the examination is limited to either technique alone.     

如何界定"治安"的含义

从治安的词源、语词、专业术语等方面考证,现代意义上的治安包含三个层面一是治安秩序;二是治安问题;三是治安工作.     

走之底笔顺识别方法

目的：建立走之底笔顺的科学识别方法。方法：收集实验笔迹样本,由样本书写人报告笔顺,依据形态对样本字分类,归纳各种形态与笔顺间的规律。结果：共获得188份有效样本,书写人报告了3种走之底笔顺,走之底折笔的收笔动向、被包围部件的位置、点笔与折笔的联系等方面可以作为识别笔顺的依据。结论：建立了走之底规范笔顺、通用笔顺和特殊笔顺的识别规则。依据这套规则。可以准确识别40％以上的不连笔走之底的笔顺。     

Sequence polymorphisms at the DXS6789, DXS8377 and DXS101 loci in three Asian populations

Sequence analyses of X-chromosomal short tandem repeats, DXS6789, DXS8377 and DXS101 were performed for representatives of 3 Asian populations: 130 Japanese, 61 Bangladeshi and 89 Indonesian males. At DXS6789, the sequence polymorphism was found in 7 alleles in the Japanese, 3 in the Bangladeshis and 3 in the Indonesians. At DXS8377, the sequence polymorphism was found in 13 alleles in the Japanese, 9 in the Bangladeshis and in all alleles identified in the Indonesians. At DXS101, the sequence polymorphism was found in 7 alleles in the Japanese, 9 in the Bangladeshis and 8 in the Indonesians. Because sequence polymorphisms were found in most of the alleles at the DXS6789, DXS8377 and DXS101 loci, it was concluded that sequencing was essential for identifying the alleles at these loci in all 3 Asian populations.     

4株猪瘟流行野毒株病毒结构蛋白E2基因的核苷酸序列分析

应用RT-PCR和nPCR扩增由4株甘肃省近期(1997-1998)猪瘟流行野毒株的E2基因,将其克隆到pGEMTEasy载体上,经转化、筛选、鉴定后,测出核苷核序列.4株流行毒株的E2基因核苷酸序列同源性为89.2%-99.7%,相应的氨基酸序列同源性为93.8%-99.0%.这4株流行毒株;与C-株(疫苗种毒)E2基因的核苷酸序列同源性为82.2%-84.3%,相应的氨基酸序列同源性为87.9%-90.2%,表明近期猪瘟流行毒株与C-株的gp55蛋白之间存在一定的差异.     

Unleashing novel STRS via characterization of genome in a bottle reference samples

The higher level of multiplexing possible with current sequencing technologies encourages adoption of additional STR loci to aid in mixture interpretation [1]. However, characterization of these loci and orientation on the human genome is vital for interlaboratory comparability and databasing. Currently, when a laboratory publishes population data from a locus not previously characterized for forensic use, there is no robust way to verify the locus designation, repeat region format, and fidelity of target. To address this, we have evaluated short- and long-read sequence data generated for reference materials included in the Genome in a Bottle Consortium (GIAB) [2] with the goal of reporting STR sequences for loci which may be of interest to the forensic community. Initially, we have analyzed GIAB data using Marshfield sets of primers (published in [3]), targeting over 600 microsatellite loci with STRaitRazor 3.0 [4]. In the future, this approach can be expanded to include other loci of interest. High-confidence STR sequence data will be made publicly available via GenBank record creation within the STRSeq BioProject [5]. As the cell lines represented in GIAB reference materials are available for purchase, this STR dataset represents a robust method for researchers to confirm targeted loci.     

Unusual FGA and D19S433 off-ladder alleles and other allelic variants at the STR loci D8S1132, vWA, D18S51 and ACTBP2 (SE33)

A very short FGA allele *14 and a long D19S433 allele *19.2 were detected and sequenced, as well as the new D8S1132 alleles *12.1, *14 and *15.1. Further new sequence data (vWA allele *18.3, D18S51 allele *11.2, SE33 alleles *24.2, *32, *34 and *37, including the rare variant allele *13.2) are described.     

Comparative study of D1S80 typing by capillary electrophoresis and sequencing

The D1S80 locus is very useful for personal identification in Japan. To obtain a correct allele over 45, we examined PCR amplification product of the allele over 45 both by direct sequencing and fragment analysis using capillary electrophoresis. Direct sequencing finally determined the allele as being 57. However, it was calculated to be an allele of 56 by comparison with size markers for capillary electrophoresis. The difference could be attributed to the electrophoretic size markers. This finding indicates that the direct sequencing may be useful to determine the allele over 45 in the D1S80 locus.     

Further allelic variation at the STR-loci ACTBP2 (SE33), D3S1358, D8S1132, D18S51 and D21S11

ACTBP2 (SE33), D3S1358, D8S1132, D18S51 and D21S11 are frequently used STR-loci in the forensic field. This study reports sequence data of further new or rare alleles at these loci, varying in length or in sequence, which were detected in course of investigations for various purposes.