鸡myostatin基因成熟区段DNA的克隆及其在甲醇酵母中的融合表达

根据GenBank中鸡myostatin(AF019621)成熟区段的cDNA序列设计了1对引物,利用PCR技术扩增了萧山鸡myostatin的成熟区段,构建了重组真核表达载体myostatin-pPIC9K,并在甲醇酵母中融合表达了myostatin蛋白。结果发现,萧山鸡myostatin基因存在2处碱基变异:T204→C204(编码的氨基酸没有变),C205→T205(编码的氨基酸由Pro变成Ser),从而产生了1个EspⅠ或Bpu1102Ⅰ限制性内切酶酶切位点。核苷酸序列同源性分析表明,myostatin基因成熟区段DNA在不同物种间具有高度的保守性。SDS-PAGE鉴定结果表明,表达的目的蛋白(26 ku)具有生物学活性。     

论价值冲突与犯罪

美国犯罪学家柯布林和米勒提出的价值冲突论,为我们深入研究犯罪问题提供了新的视角。价值冲突是导致行为失范进而引发犯罪的直接原因。我国转型时期的价值冲突主要表现为传统与现代的价值冲突、经济与道德的价值冲突、公平与效率的价值冲突、个体与整体的价值冲突等。面对激烈的价值冲突,只有从各方面大力提高文化融合能力,才能有效地控制犯罪的发生。     

论南诏的民族政策

南诏是一个多民族集合体政权。其对辖地各民族的治策、移民政策和发展辖地各民族经济交流的政策,具有实用性、复杂性和灵活性的特点。南诏的民族政策,既巩固和发展了南诏的统治,也促进了云南及周边地区的社会发展和各民族的交往。南诏的民族政策也有局限性,主要表现在居于支配地位的白蛮和乌蛮贵族对其他民族进行压迫和掠夺。     

包容的价值取向与包容性增长的融合途径探析

包容的价值取向作为一种价值导向和发展观,在人的发展和社会主义核心价值体系建设中发挥着越来越大的作用,包容性增长是包容的价值取向在经济领域的投射。但随着经济社会的发展,包容性增长中重视效率和效益的问题被放大,掩盖了其所蕴含的社会包容性发展问题。因此,用包容性发展的概念取代包容性增长的概念,并实现包容的价值取向与包容性增长的融合便成为重要的研究课题。本文在论述二者融合的必要性的基础上,探讨了二者融合的途径,以期用二者的融合促进人与社会的包容以及人的和谐发展、全面发展。     

新城疫病毒融合蛋白抗体间接ELISA检测方法的建立

用新城疫病毒(NDV)F48E8株作为包被抗原,建立了检测由鸡痘病毒载体介导表达的NDV融合蛋白(F)抗体的间接ELISA方法.通过对20份阳性血清的P/N值与ELISA抗体效价(ET)的自然对数值(lnET)的线性回归分析,获得了回归方程lnET =0.201×P/N+4.632.应用该ELISA方法对表达NDV F基因的重组鸡痘病毒免疫鸡群进行了血清抗体检测,结果表明,血清的ELISA抗体效价与相应血清中和试验的抗体效价高度相关.攻毒保护试验的结果显示,ELISA抗体效价与机体的保护力表现一定程度的相关性.     

The role of people in information-sharing: perceptions from an analytic unit of a regional fusion center

Information sharing research has largely focused on capturing operational practices related to the exchange of information. A noticeable shortcoming of this developing literature is the lack of scholarly attention focused specifically on analytic personnel. The present study employs semi-structured interviews with 23 personnel that comprise the analytic unit within a regional fusion center located in a large metropolitan area in the United States. Findings indicate that there is significant ambiguity surrounding the organizational and geographic audiences to which intelligence products should be tailored. Moreover, it appears information sharing is sparse and reliant upon personal relationships between analysts and external organizations. Lastly, analysts do not receive feedback pertaining to the content and effectiveness of intelligence products created. These findings may be generalized to other analytical organizations whose primary goal is to gather, analyze and disseminate intelligence. Action steps for policymakers are provided.     

论法律移植与法律融合

在中外法制史上，法律移植与法律融合是非常普遍的现象，是各国法律建设中的一个重要的内容。印度和日本移植外来法的政治、社会、文化奈件不同，所走的道路也存在较大差异，但比较两国移植外来法，改造本土法，使两者并存融合的过程，仍可总结成功的经验和共同的规律。     

Multi-resolution image fusion technique and its application to forensic science

Image fusion is a process of combining two or more images into an image. It can extract features from source images, and provide more information than one image can. Multi-resolution analysis plays an important role in image processing, it provides a technique to decompose an image and extract information from coarse to fine scales. In some practical forensic examinations (such as the cartridge image check), we cannot obtain all information from just one image; on the contrary, we need information from images with difference light sources (or light ways). In this paper, we apply an image fusion method based on multi-resolution analysis to forensic science. Synthetic and real images (such as images from closed-up photography and flash photography) are used to show the capability of the multi-resolution image fusion technique.     

跨文化传播时代的广告

广告文化作为文化传播的一种形式,必须为弘扬民族文化、实现民族文化的合理拓展作出承诺。从广告传播的角度来看,广告若欲将信息传递给目标受众,就必须采用本民族容易接受的文化形式和文化价值观。因此,在广告传播中采用民族题材、弘扬民族文化价值观的广告作品十分多见。这些广告表现了同一文化的亲和力,极大的唤起了消费者的文化认同,从而导致其购买行为,而客观上它们又弘扬了民族传统。     

Comparison of feature selection and data fusion of Fourier transform infrared and Raman spectroscopy for identifying watercolor ink

The identification of different kinds of watercolor inks is an important work in the field of forensic science. Four different kinds of watercolor ink Spectroscopy data fusion strategies (Fourier Transform Infrared spectroscopy and Raman spectroscopy) combined with a non-linear classification model (Extreme Learning Machine) were used to identify the brand of watercolor inks. The study chose Competitive Adaptive Reweighted Sampling (CARS), Random Frog (RF), Variable Combination Population Analysis-Genetic Algorithm (VCPA-GA), and Variable Combination Population Analysis-Iteratively Retains Informative Variables (VCPA-IRIV) to extract characteristic variables for mid-level data fusion. The Cuckoo Search (CS) algorithm is used to optimize the extreme learning machine classification model. The results showed that the classification capacity of the mid-level fusion spectra model was more satisfactory than that of single Infrared spectroscopy or Raman spectroscopy. The CS-ELM models based on infrared spectroscopy used to recognize the watercolor ink according to brands (ZHENCAI, DELI, CHENGUANG, and STAEDTLER) obtained an accuracy of 66.67% in the test set using all spectral datasets. The accuracy of CS-ELM models based on Raman spectroscopy was 67.39%. The characteristic wavelength selection algorithms effectively improved the accuracy of the CS-ELM models. The classification accuracy of the mid-level spectroscopy fusion model combined with the VCPA-IRIV algorithm was 100%. The data fusion method increased effectively spectral information. The method could satisfactorily identify different brands of watercolor inks and support the preservation of artifacts, paintings, and forensic document examination.