贪污罪量刑基准体系的构建及回归分析

根据刑法规定,通过贪污罪的犯罪结果与处刑数量之间的线性关系,把定量分析方法应用于贪污罪的处刑实践,并依此建立了贪污罪的量刑基准体系,以准确确定被告人应当承担刑事责任的数量,以减少司法实践中法官个体之间对贪污罪行为处刑的差异。     

国家公务员考核量化测评方法研究

建立科学的分类考核要素和量化测评体系,是弱化主观因素的影响、提高考核结果科学性、准确性的关键之一。为解决国家公务员考核量化测评过程中主观因素影响较大的问题,可运用层次分析法与模糊评判法来促进国家公务员考核定量化。在重视与现行公务员考核量化测评方法衔接的基础上,通过适当补充、完善考核要素指标,采用层次分析法(AHP)确定一级考核指标因素和二级考核因子的权值,以建立模糊综合测评的模型。     

Smart and secure borders through automated border control systems in the EU? The views of political stakeholders in the Member States

The European Commission launched the “Smart Borders” policy process in 2011 to enhance border security in the European Union (EU) using technologisation and harmonisation. This includes the use of automated border control (ABC) systems. The Member States crucially shape the process, weighing security technologies and costs, privacy and rights, and further institutional choices. We examine the views of political stakeholders in four Member States by conducting a systematic empirical and comparative study unprecedented in the existing, political-theory-inspired research. In our Q methodological experiments, political stakeholders in Finland, Romania, Spain and the UK rank-ordered a sample of statements on Smart Borders, ABC and harmonisation. The factor analysis of the results yielded three main views: the first criticising ABC as a security technology, the second welcoming the security gains of automation and the third opposing harmonised border control. While impeding harmonisation, the results offer a consensus facilitating common policy.     

犯罪心理学研究中运用量化方法引起的理论思考

量化与统计分析是一项重要的研究方法,在犯罪心理学研究中得到了广泛运用,同时也存在着使用的局限性。犯罪人有很强的心理掩饰性,对包括犯罪人在内的人的心理的测量实际上是一种间接测量,测得的数据是心理学家标定的数据,兼有客观性与主观性。量化研究如果不与科学思维相结合,有可能得出浅薄平庸甚至错误的结论。心理学研究应当以“人性”为基点,多种方法综合运用,实现量化方法与非量化方法的有机结合。     

CELO病毒fiber1基因部分片段的原核表达及抗原性鉴定

根据鸡胚致死孤儿(CELO)病毒Phelps株的fiber1基因(GenBank序列号U46933)序列设计了1对引物,采用PCR扩增CELO病毒fiber1基因C端(包含Knobs区)的部分片段,并将其克隆到质粒表达载体pGEX6p1,获得的阳性克隆命名为pGEXAF807。序列分析表明,所获得的DNA片段核苷酸序列与CELO病毒Phelps株的fiber1基因序列完全一致。将pGEXAF807转化大肠埃希氏菌DH5α,经37℃、0.6mmol/LIPTG诱导表达5h,SDSPAGE分析超声裂解后的上清和沉淀。结果显示,目的蛋白以包涵体形式存在,蛋白分子质量约为54.6ku,目的蛋白量占菌体总蛋白的35.7%。用CELO病毒多克隆血清做Westernblotting试验,结果表明目的蛋白具有较好的抗原活性。     

Implementation of a robotized real-time PCR setup for the use of the Quantifiler™ Human DNA Quantification Kit

Human DNA quantification occupies a central role within the DNA analytic process of forensic casework samples as DNA quantification results have an important impact on the quality of the short tandem repeat data. Manual processing for the setup of quantification reactions can be time consuming and labor intensive. Therefore automation of quantitative real-time PCR setup was an important component of our DNA-analysis automation concept. Here we show the implementation of a robotized setup for the Quantifiler™ Human DNA Quantification Kit.     

Developmental Validation of the Quantifiler® Duo DNA Quantification Kit for Simultaneous Quantification of Total Human and Human Male DNA and Detection of PCR Inhibitors in Biological Samples*

Abstract: The Quantifiler^® Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. Pooled human male genomic DNA is used to generate standard curves for both human (ribonuclease P RNA component H1) and human male (sex determining region Y) specific targets. A shift in the cycle threshold (C_T) values for the internal positive control monitors the presence of PCR inhibitors in a sample. The assay is human specific and exhibits a high dynamic range from 0.023 to 50 ng/μL. In addition, the multiplex assay can detect as little as 25 pg/μL of human male DNA in the presence of a 1000-fold excess of human female DNA. The multiplex assay provides assessment of the DNA extract and guidance for the selection of the appropriate AmpFℓSTR^® Amplification Kit to obtain interpretable short tandem repeat profiles.     

试论道路交通事故责任量化的可能性

道路交通事故责任是追究当事人法律责任的依据。鉴于目前交通事故责任在认定和划分上都存在一定的缺陷，有必要对其进行近一步的完善。     

付立叶红外显微镜及其附件检验微量纤维

通过付立叶红外显微镜及其附件ATR、金刚石池对微量纤维进行检验,从而达到对微量或痕量纤维准确确定其种属和比对检验是否相同的目的,方法准确、快捷、可靠.     

贪污罪量刑基准体系的构建及回归分析

应当根据刑法规定,通过贪污罪的犯罪结果与处刑数量之间的线性关系,把定量分析方法应用于贪污罪的处刑实践,并依此建立了贪污罪的量刑基准体系,以准确确定被告人应当承担刑事责任的数量,这样,可以减少司法实践中法官个体之间对贪污罪行为处刑的差异。