中国汉族与日本群体DYFl55S1基因座的遗传多态性

目的探讨Y染色体DYF155S1基因座的遗传多态性及群体间差异。方法 应用MVR-PCR、荧光显谱及DNA序列分析技术,对来自中国群体(北方汉族,64例)和日本群体(43例)男性个体的DYF155S1基因座进行初步分析。结果107例样本共检出了5种重复序列类型,包括新的命名为6型的重复序列,它是在1型的基础上T22A置换所形成,仅存在于日本群体,可作为民族特征性遗传标记。2群体重复序列的排列方式以3134顺序为主,在中国和日本群体中各占73.44％和67.44％,是黄种人的特点。134顺序在中国群体中占第二位,为17.19％,6134排列占日本群体的16.28％。3’端的4型重复序列的平均数目在日本群体为8.8条,明显低于中国群体的12.5条。结论DYF155S1基因座具有非常高的遗传多态性和明显的群体差异。     

黄芪三仙汤通过海马神经肽Y调节细胞免疫功能的实验研究

目的:探讨黄芪三仙汤调节细胞免疫功能作用机制。方法:将24只雄性大鼠随机分为假手术组、模型组和治疗组。将1μlβ-淀粉样蛋白(Aβ)1-40(10μg/μl)在立体定向仪下注入模型组和治疗组大鼠右侧Meynert核复制阿尔茨海默病(AD)模型,治疗组用黄芪三仙汤灌胃,于4周末观察各组海马区神经肽Y(NPY)、血浆中促肾上腺皮质激素(ACTH)、血清中皮质酮(CORT)含量,胸腺指数,脾脏巨噬细胞数目的变化。结果:模型组大鼠海马组织中NPY的释放减少,血浆中ACTH和血清中CORT含量显著降低,脾脏巨噬细胞数目显著增加(P<0.01)。治疗组NPY的释放增加,血浆中ACTH和血清中CORT含量显著升高,脾脏巨噬细胞数目明显减少(P<0.01)。结论:黄芪三仙汤可以通过海马-NPY-垂体-肾上腺轴调节细胞免疫功能。     

新Y-STR基因座DYS709在汉族人群中的遗传多态性调查

目的筛选新的Y-STR基因座,调查其在汉族人群中的等位基因频率分布,评价其在法医学及其它方面的应用价值。方法在Y染色体基因组DNA中查找候选基因座,在重复顺序两端设计引物,PCR扩增后用银染法显示结果。结果一个重复单位为CTTT的Y-STR基因座DYS709被发现。在102例汉族无关男性个体血样中共检出了7个等位基因。基因多样性为0.7063,个人识别能力(PD)和非父排除率(PE)均为0.7063。结论新筛选到的DYS709具有较高的遗传多态性,在法医学及人类遗传学方面具有应用价值。     

关于转型期企业共青团建设的几点思考

企业团组织引导青年发展,应基于青年特点和企业的实际展开。企业团组织要发挥政治上引领青年、帮助青年成才、对青年的考核等方面的作用。企业团建工作要从健全组织机构、介入企业文化、推进组织创新、规范组织生活、开展青年养成教育等方面入手。     

Y染色体单核苷酸多态性及其在刑事侦查中的应用价值

寻找和研究SNP已成为后基因组时代研究的重要内容和目标。Y染色体的拟常染色体非重组区段的SNP标记,是目前公认的研究早期人类进化和迁移以及法医学应用最理想的工具。Y染色体单核苷酸多态性（SNP）分型和检测方法的发展,决定了它能被广泛运用于刑事侦查当中。Y染色体的命名、标记和分类等技术分析方法有待进一步地发展,以提高其识别的规范性与稳定性。     

多种用工制度下国有企业团工作面临的困难及对策思考

国有企业多种用工制度并存的条件下，共青团工作面临着企业青年职业状态复杂化、思想观念多元化、利益诉求多样化，以及团的组织建设与管理、团的工作与活动开展困难等诸多问题与挑战。解决现有多种用工制度下国企团工作中的困难和问题的主要思路与方法是：以青年为本，竭诚服务青年，促进青年与企业和谐发展，进一步解放思想，突破体制束缚，创造性地开展企业共青团工作。     

Y chromosome J2 subtyping in an Italian sample: Population and forensic implications

In the recent years the Y chromosome genealogy has been refined by a number of newly discovered SNPs. The non-random distribution of the Y chromosome lineages worldwide makes fundamental the dissection and characterisation of haplogroups associated with specific geographic areas. In Southern Europe the haplogroup J2, as defined by the M172 marker, can reach frequencies up to 35%, making the dissection of such lineage critical for population studies. Here we present a study on J2 chromosomes from the Italian peninsula. Populations and forensic implications are discussed. A total of 900 individuals were previously genotyped for a number of SNPs, including M172. More than 200 of these have been now genotyped for 7 SNPs within the J2 lineage using a multiplex SNaPshot approach. The different distribution of the various lineages in different geographic areas probably reflects different historical demographic events and points to differential Y chromosome haplotype distribution, with implication for forensic application of this genetic marker.     

Developmental Validation of the Quantifiler® Duo DNA Quantification Kit for Simultaneous Quantification of Total Human and Human Male DNA and Detection of PCR Inhibitors in Biological Samples*

Abstract: The Quantifiler^® Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. Pooled human male genomic DNA is used to generate standard curves for both human (ribonuclease P RNA component H1) and human male (sex determining region Y) specific targets. A shift in the cycle threshold (C_T) values for the internal positive control monitors the presence of PCR inhibitors in a sample. The assay is human specific and exhibits a high dynamic range from 0.023 to 50 ng/μL. In addition, the multiplex assay can detect as little as 25 pg/μL of human male DNA in the presence of a 1000-fold excess of human female DNA. The multiplex assay provides assessment of the DNA extract and guidance for the selection of the appropriate AmpFℓSTR^® Amplification Kit to obtain interpretable short tandem repeat profiles.     

Developmental validation of the Microreader™ Y Prime Plus ID System: An advanced Y-STR 38-plex system for forensic applications

Y-STR is widely used in sexual assaults and familial searches of suspects. Here, we reported a novel 38-plex STR genotyping system designed for forensic applications. Microreader? Y Prime Plus ID System (YPP) amplifies 38 loci in one reaction, including 29 loci from commonly used Yfiler® Plus PCR Amplification Kit & PowerPlex® Y23 System (DYS393, DYS570, DYS19, DYS392, DYS549, Y GATA H4, DYS460, DYS458, DYS481, DYS635, DYS448, DYS533, DYS449, DYS456, DYS389I, DYS390, DYS389Ⅱ, DYS438, DYS391, DYS439, DYS437, DYS385a/b, DYS643, DYS518, DYS576, DYF387S1a/b, and DYS627), 6 commonly used loci for the Y-STR database (DYS444, DYS447, DYS596, DYF404a/b, DYS527a/b, DYS557) and one Y-indel specific for the Chinese population. YPP is designed for different types of samples, such as blood card and swabs. In this work, YPP was validated following SWGDAM guidelines (2016) and guidelines from Ministry of Public Security of the People’s Republic of China, including PCR-based, sensitivity, accuracy and precision, mixture, stability and inhibitor, and species specificity. The results indicate that the Microreader? Y Prime Plus ID System is a powerful identification kit designed for forensic databases.     

9个Y-STR基因座荧光复合扩增系统的法医学应用

目的建立9个Y-STR基因座的复合扩增系统,提高Y-STR的法医学检测效能。方法6-FAM标记DYS434、Y-GATA-A10、DYS438、DYS439,HEX标记DYS531、DYS557、DYS448,TAMRA标记DYS456、DYS444引物,PCR复合扩增,毛细管电泳得到结果,考察扩增系统的个体识别能力、灵敏度、特异性、组织同一性。结果所建立的9个Y-STR复合扩增系统分型清晰,单倍型多样性达0.9968,特异性好,灵敏度高(0.5ngDNA),并且在男女混合斑检验上较常染色体STR分型更有优势。结论9个Y-STR复合扩增系统具有较高的识别能力,对建立Y染色体STR数据库,研究群体遗传学和进行法医学混合斑物证鉴定有重要意义。