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应用提纯的雏鹅病毒性肠炎病毒(NGVEV)抗原制备抗原诊断膜,建立了IgG的金颗粒标记、抗原抗体反应同步试验的斑点免疫金染色(DotIGS)检测雏鹅新型病毒性肠炎(NGVE,暂定)抗体的方法,只需30~40min即可判断结果;与本动物抗GPV,DPV,NDV,IBV,ILTV,MDV,IBDV,EDSV及多杀性巴氏杆菌血清不发生交叉反应;对兔抗NGVEV的IgG的最低检出量为1.0×10-10g/mL。雏鹅感染NGVEV强毒后第3d、成年鹅接种NGVEVCN40弱毒疫苗后第3d即可检测到相应抗体。对重庆市和四川省10个县(市)的617份成年鹅血清进行检测,结果NGVE的血清阳性率为30.44%~36.84%。该法适用于雏鹅感染的早期诊断、成年鹅的血清流行病学调查和疫苗免疫效果评价等。 相似文献
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用反相杂交技术对HLA-DQA1基因分型及其应用 总被引:2,自引:0,他引:2
目的 :HLA -DQA1其因的分型研究及在实际办案中的应用。方法 :PCR结合反相杂交检测技术对HLA -DQA1基因进行分型。结果 :检见7种基因 ,24种基因型 ,获得上海地区HLA -DQA1基因的基因频率及基因型的频率分布数据。结论 :对法医学中常见的血液 (痕 )、牙齿、精斑、混合斑、人体组织等检材进行HLA -DQA1分型检测 ,其结果稳定可靠 ,为实际办案提供了科学依据 相似文献
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Om Kumar Ph.D. Santwana Pradhan M.Sc. Payal Sehgal M.Sc. Yamini Singh M.Sc. Rajagopalan Vijayaraghavan Ph.D. 《Journal of forensic sciences》2010,55(3):801-807
Abstract: Ricin is a glycoprotein from Ricinus communis seeds. It is known to have diverse toxic effects on cells of different visceral organs. In the present study, we purified and denatured ricin in a boiling water bath for different time intervals. We further made an attempt to identify native and denatured ricin by immunobased detection systems. All the antigen/antibody‐based assays identified native and denatured ricin. On SDS–PAGE, only native ricin was observed. In western blotting, ricin boiled for 3.75 min gave a strong band on X‐ray film. On native polyacryl amide gel electrophoresis, native and denatured ricin gave ricin band in 60‐kDa region. The denatured ricin did cause mortality up to 25 mg/kg, while 5 and 10 μg/kg of native ricin caused 50% and 100% mortality, respectively. Detection of native and denatured ricin is very difficult, and the investigating agencies, forensic scientists, and analysts should be very careful while interpreting the results. 相似文献
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王贵青 《云南警官学院学报》2012,(4):119-123
量子点是一种由II—VI族或III—V族元素组成的15nm以下纳米颗粒,因发射光谱颜色可调、荧光量子产率高、荧光发光稳定,寿命长的而被广泛应用于生物医学,荧光成像等领域。量子点的制备方法是有机金属合成法、水相合成法。因量子点的特性,荧光量子点材料在汗手印、血手印的检测中得到广泛应用。将荧光量子点材料应用于潜在手印显现是当今指纹技术的发展趋势之一,具有广阔的发展前景。 相似文献
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