波尔山羊卵母细胞和早期胚胎的端粒酶活性

利用端粒重复序列扩增(TRAP)法进行了山羊卵母细胞和附植前胚胎的端粒酶活性的测定;结果表明,山羊卵母细胞和早期胚胎的端粒酶活性都为阳性。TPG定量比较发现,卵母细胞的TPG最低,为25.348U;4-细胞的TPG为273.832U,至8-细胞时,TPG又降低到56.117U,随后快速升高,桑椹胚的TPG为251.111U;囊胚的端粒酶活性最高,TPG为519.46U。     

高校体育礼仪教育的功能及其实施途径

体育礼仪主要是指人们在体育交往中所应具备的相互敬重、亲善和友好的行为规范。体育礼仪的内容主要是由内在的体育礼仪文化和外在的体育礼仪行为方式或礼仪程式等组成。通过礼仪教育可以有效地提高大学生的道德素质,增进相互之间的友谊和交流,有助于构建和谐校园。因此,学校要努力营造礼仪文化教育的氛围,加大宣传力度,塑造新型的体育人格和教育关系。     

全球视野中的著作权刑法保护

著作权作为知识产权体系中的一项重要权利,已越来越为国际社会所重视,尤其是步入信息化时代后,保护著作权的重要意义更是得到了显现。我国近年来在著作权立法方面取得了一系列的突破,一方面加入了一些重要的国际公约,并承诺履行公约要求的义务;另一方面逐步完善了著作权的国内立法体系。但在如何打击侵犯著作权刑事犯罪方面,尚有诸多地方有待改善。     

People Trafficking: Conceptual issues with the United Nations Trafficking Protocol 2000

This paper examines the UN 2000 Trafficking Protocol in the context of international responses to the issue of people trafficking. Attention is drawn to the conceptual flaws in this new instrument regarding the failure to address domestic trafficking, not incorporating the purchasing and selling of people as defining characteristics of trafficking, and the lack of clarity around issues of prostitution. Framing the discussion within feminist theory, the essay concludes that women’s campaigning will continue to be crucial to putting issues such as human trafficking on the international political agenda. This is extended to affirm that people’s struggle against oppressive circumstances will lead the tackling of the underlying causes of trafficking.     

The nature, origin and impact of legally binding consequences: the case of the climate regime

The Marrakesh Accords provide a detailed compliance system for the Kyoto Protocol. An innovative feature of this system is an Enforcement Branch authorized to apply punitive measures or “consequences” in the second commitment period to Annex I Parties that have been found to be in non-compliance in the first commitment period. However, even after the latest Conference of the Parties (COP) to the United Nations Framework Convention on Climate Change (UNFCCC), COP-11, and the first COP serving as the meeting of the Parties to the Kyoto Protocol, COP/MOP-1, it is not yet clear whether these consequences will be legally binding. The purpose of this paper is three-fold. First, we examine the legal nature of the punitive consequences embedded in the Marrakesh Accords. Second, we discuss potential motives for making these consequences legally binding. We point out that one such motive is that their implementation requires cooperation by the Party that is in non-compliance. In this regard, Kyotoȁ9s compliance system differs from other international compliance systems equipped with punitive consequences, such as those of the WTO and the UN. Finally, we consider whether making the punitive consequences legally binding is likely to make a difference. The conclusion, which should be of interest to both academic researchers and the policy community, is that the legal status of the consequences is likely to have only a modest effect on compliance levels. A country that deliberately fails to abide by other legally binding commitments under the Kyoto Protocol is also likely to resist the application of punitive consequences, regardless of whether these consequences are made legally binding or not.     

A review of the current understanding of burned bone as a source of DNA for human identification

Identification of incinerated human remains may rely on genetic analysis of burned bone which can prove far more challenging than fresh tissues. Severe thermal insult results in the destruction or denaturation of DNA in soft tissues, however genetic material may be preserved in the skeletal tissues. Considerations for DNA retrieval from these samples include low levels of exogenous DNA, the dense, mineralised nature of bone, and the presence of contamination, and qPCR inhibitors. This review collates current knowledge in three areas relating to optimising DNA recovery from burned bone: 1) impact of burning on bone and subsequent effects on sample collection, 2) difficulties of preparing burned samples for DNA extraction, and 3) protocols for bone decalcification and DNA extraction. Bone decalcification and various DNA extraction protocols have been tested and optimised for ancient bone, suggesting that prolonged EDTA (Ethylenediaminetetraacetic acid) demineralisation followed by solid-phased silica-based extraction techniques provide the greatest DNA yield. However, there is significantly less literature exploring the optimal protocol for incinerated bones. Although burned bone, like ancient and diagenetic bone, can be considered “low-copy”, the taphonomic processes occurring are likely different. As techniques developed for ancient samples are tailored to deal with bone that has been altered in a particular way, it is important to understand if burned bone undergoes similar or different changes. Currently the effects of burning on bone and the DNA within it is not fully understood. Future research should focus on increasing our understanding of the effects of heat on bone and on comparing the outcome of various DNA extraction protocols for these tissues.     

Measures to enhance the success of global climate treaties

We analyze important forces that hamper the formation of successful self-enforcing agreements to mitigate global warming from an economic point of view. The analysis combines two modules: (a) an integrated assessment model that captures the feedback between the economy, environmental damages and the climate system and (b) a game theoretic model that determines stable coalitions in the presence of free-riding incentives. We consider two types of measures to enhance the success of international environmental treaty-making: (a) transfers, aiming at balancing asymmetric gains from cooperation; (b) institutional changes, aiming at making it more difficult to upset stability of a treaty. We find that institutional changes may be as important as transfers and should therefore receive more attention in future international negotiations.

防火墙技术分析

Internet的迅速发展给现代人的生产和生活都带来了前所未有的飞跃,大大提高了工作效率,丰富了人们的生活,弥补了人们的精神空缺;而与此同时给人们带来了一个日益严峻的问题——网络安全。网络的安全性成为当今最热门的话题之一,很多企业为了保障自身服务器或数据安全都采用了防火墙。随着科技的发展,防火墙也逐渐被大众所接受。本文阐述了防火墙工作的方式,以及防火墙的基本分类,并且讨论了每一种防火墙的优缺点。     

针刺持续时间及刺激方案对针刺脑功能成像的影响

目的 利用BOLD-fMRI技术，在保持其他影响因素不变的情况下研究针刺持续时间及针刺刺激方案对脑功能成像激活区定位的影响。方法 选取健康志愿者40例，平均分为2组，为持续时间组及刺激方案组，第1组采用相同的针刺刺激方案（改良组块设计）进行3轮（R1、R2、R3）功能像数据采集，第2组采用3种不同的针刺刺激方案（包括典型组块、改良组块设计及事件相关设计）进行3轮功能像采集，两组数据均采用相同的MR扫描参数，用AFNI软件进行数据处理，用Monte Carlo阈值校正方法确定脑功能激活区，得出每组的组内分析结果及组内不同轮次比较的结果。 结果 持续时间组及刺激方案组的组内fMRI信号存在明显差异。组内不同轮次fMRI信号比较也均存在显著差异的脑区，两组的R2与R1相比均存在fMRI信号减低区域，但刺激方案组的R3与R1相比存在广泛的fMRI信号减低区域，持续时间组的R3与R1相比存在fMRI信号增高区域。 结论 针刺持续时间及刺激方案均会对脑功能成像的激活区定位产生影响。