| 应用PCR-RFLP分析鉴别广西鸡毒霉形体 |
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| 引用本文： | 邓显文,谢芝勋,谢志勤,唐小飞,刘加波,廖敏,庞耀珊.应用PCR-RFLP分析鉴别广西鸡毒霉形体[J].中国兽医科学,2004,34(3):41-44. |
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| 作者姓名： | 邓显文 谢芝勋 谢志勤 唐小飞 刘加波 廖敏 庞耀珊 |
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| 作者单位： | 广西兽医研究所,广西,南宁,530001 |
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| 基金项目： | 广西留学基金 (桂科回 0 0 0 90 0 1) |
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| 摘 要： | 针对鸡毒霉形体 (MG) 16S和 2 3SrRNA基因间的高变区序列设计合成了 1对引物 ,对MG菌株进行了PCR扩增 ,获得了 1条 899bp的DNA片段 ,而其他禽病病原DNA无扩增。扩增产物经用限制性内切酶AfaⅠ和DraⅠ进行酶切片段长度多态性分析 ,表明各MG菌株酶切图谱间均存在差异。
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| 关 键 词： | 鸡毒霉形体 PCR 限制性片段长度多态性 广西 |
| 文章编号： | 1000-6419(2004)03-0041-04 |
| 修稿时间： | 2003年11月14 |
Analysis and identification of Mycoplasma gallisepticum from Guangxi Region by PCR-RFLP |
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| DENG Xian-wen,XIE Zhi-xun,XIE Zhi-qin,TANG Xiao-fei,LIU Jia-bo,LIAO Min,PANG Yao-shan.Analysis and identification of Mycoplasma gallisepticum from Guangxi Region by PCR-RFLP[J].Veterinary Science in China,2004,34(3):41-44. |
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| Authors: | DENG Xian-wen XIE Zhi-xun XIE Zhi-qin TANG Xiao-fei LIU Jia-bo LIAO Min PANG Yao-shan |
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| Abstract: | According to Mycoplasma gallisepticum (MG) hypervariable region between 16 S rRNA and 23 S rRNA, a pair of primers was designed. The MG DNA fragment 899 bp in length was specifically amplified by means of using the primers. The PCR product was analysed by the restriction fragment length (polymorphism) (RFLP) method. The results indicated that the RFLP patterns of the amplified fragment were different. The current study may play important role in MG epidemiological investigation in Guangxi Region. |
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| Keywords: | PCR restriction fragment length polymorphism (RFLP) Mycoplasma gallisepitcum |
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