血尿肝中毒鼠强硅藻土提取GC／NPD检测法

目的建立硅藻土提取气相色谱测定血、尿、肝中毒鼠强的方法。方法原尿液、血液用水稀释、肝匀浆用6％高氯酸沉淀蛋白的上清液倒入硅藻土小柱中，血和尿用苯洗脱，肝用三氯甲烷洗脱，挥干洗脱液，用甲醇定容至0．1ml。结果血提取率98．4％，尿提取率95．6%，肝提取率98．1％。相对标准偏差低于3．2％，检出限低于20ng／ml（g）。结论该法简便、快速，提取率高，适合作为常规毒物分析方法。

Determination of tetramine in blood, urine and liver by solid-phase extraction with celite and GC/NPD

Objective To establish a simple and rapid method for the determination of tetramine in blood, urine and liver by solid- phase extraction with celite and GC/NPD. Methods Blood diluted with water or urine or supernant of the homogenized liver deproteined with 6% perchloric acid were added to celite column and the elutions were effected with benzene for blood and urine, or chloroform for liver. The eluations were evaporated on water bath. The dried residues were redissolved with 0.1ml methanol respectively. Results Limit of detection for tetramine is below 20ng/g（ml）. The recoveries of tetramine were 98. 4% for blood, 95. 6% for urine and 98. 1% for liver. Conclusion The method can be used in casework for determination of tetramine in blood, urine and liver.