| J亚群禽白血病病毒膜表面糖蛋白基因gp85的克隆与表达 |
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| 引用本文： | 杨玉莹,秦爱建,赵振华,叶建强,顾玉芳,王金铃.J亚群禽白血病病毒膜表面糖蛋白基因gp85的克隆与表达[J].中国兽医科学,2003,33(7):3-6. |
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| 作者姓名： | 杨玉莹 秦爱建 赵振华 叶建强 顾玉芳 王金铃 |
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| 作者单位： | 1. 内蒙古农业大学,内蒙古,呼和浩特,010018
2. 扬州大学,江苏,扬州,225009 |
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| 摘 要： | 以pGEM IMC2 .2 重组质粒为模板扩增禽白血病病毒内蒙株IMC10 2 0 0 gp85基因 ,构建了转移载体pFastBacl IMCgp85 ,并利用Bac to Bac表达系统获得了重组杆状病毒rBac IMCgp85。转染Sf 9细胞后的表达研究表明 ,重组杆状病毒可高效表达IMC10 2 0 0 的gp85基因 ,表达产物具有病毒的抗原特异性 ,与ALV J单抗JE 9有强的间接免疫荧光反应。蛋白质印迹法分析表明 ,表达产物的分子质量约 5 4ku。
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| 关 键 词： | 禽白血病病毒 J亚群 gp85基因 克隆 表达 |
| 文章编号： | 1000-6419(2003)07-0003-04 |
| 修稿时间： | 2003年2月12日 |
Cloning and expression of env-gp85 of ALV-J IMC10200 strain |
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| YANG Yu ying ,QIN Ai jian ,ZHAO Zhen hua ,YE Jian qiang ,GU Yu fang ,WANG Jin ling.Cloning and expression of env-gp85 of ALV-J IMC10200 strain[J].Veterinary Science in China,2003,33(7):3-6. |
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| Authors: | YANG Yu ying QIN Ai jian ZHAO Zhen hua YE Jian qiang GU Yu fang WANG Jin ling |
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| Institution: | YANG Yu ying 1,QIN Ai jian 2,ZHAO Zhen hua 1,YE Jian qiang 2,GU Yu fang 1,WANG Jin ling 1 |
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| Abstract: | This report described the cloning and expression of envelope gene gp85 of IMC 10200 strain of avian leukosis virus subgroup J (ALV J). The sequence encoding the gp85 domain of ALV J IMC 10200 strain was amplified from pGEM IMC 2.2 vector, and cloned into vector pFast Bacl. By the Bac to Bac baculovirus expression system, rBac IMC gp85 was obtained. Immunofluorescence assay with JE 9 monoclonal antibody against ALV J showed that the recombinant gp85 gene product was expressed in Sf9 cells infected with rBac IMC gp85. The molecular weight of expressed protein was about 54 ku by western blotting. |
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| Keywords: | avian leukosis virus subgroup J gp85 gene clone expression |
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