| 薄层色谱法检测丁丙诺啡 |
| |
| 引用本文： | 刘冬娴,贺江南.薄层色谱法检测丁丙诺啡[J].中国法医学杂志,2007,22(4):242-244. |
| |
| 作者姓名： | 刘冬娴 贺江南 |
| |
| 作者单位： | 湖南省公安高等专科学校,湖南,长沙,410006 |
| |
| 摘 要： | 目的建立丁丙诺啡薄层色谱分析方法。方法血浆先用乙醇除蛋白,尿、水剂样品则直接加pH10.8Na2CO3/NaHCO3缓冲溶液后用氯仿提取,提取液经净化浓缩后进行薄层色谱分析。结果选定碘化铋钾作显色剂,丁丙诺啡薄层色谱分析展开效果较好,检测灵敏度为0.5μg,血浆、尿液和水剂中检出限分别为0.7、0.7和0.6μg/ml。结论该方法适用于涉毒案件中丁丙诺啡的日常检验。
|
| 关 键 词： | 法医毒物学 薄层色谱 丁丙诺啡 血浆 尿 水样 |
| 文章编号： | 1001-5728(2007)04-0242-03 |
| 修稿时间： | 2006年8月14日 |
Determination of buprenorphine by thin layer chromatography |
| |
| LIU Dong-xian,HE Jiang-nan.Determination of buprenorphine by thin layer chromatography[J].Chinese Journal of Forensic Medicine,2007,22(4):242-244. |
| |
| Authors: | LIU Dong-xian HE Jiang-nan |
| |
| Abstract: | Objective A rapid and inexpensive method,based on thin-layer chromatography,is described for the qualitative determination of buprenorphine.Methods After adding Na2CO3/NaHCO3 buffer solution(pH 10.8),buprenorphine in specimen was extracted with chloroform.Extract phase was refined and concentrated,then entirely added on the silica-gel GF254 plate.Potassium iodobismuthater solution was used to show analyee on the plate.Results The limits of buprenorphine in plasma,urine and aqua were 0.7,0.7 and 0.6μg/ml respectively.Conclusion The method is suitable for routine laboratory detemination of buprenorphine for the dope cases. |
| |
| Keywords: | Forensic toxicology Thin layer chromatography Buprenorphine Plasma Urine Aqua |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
