| 旋毛虫单克隆抗体特性鉴定 |
| |
| 引用本文： | 王克领,李春华,马增全,阎玉河,袁文甫,许威光.旋毛虫单克隆抗体特性鉴定[J].中国兽医科学,1999(8). |
| |
| 作者姓名： | 王克领 李春华 马增全 阎玉河 袁文甫 许威光 |
| |
| 作者单位： | 河南省农业科学院畜牧兽医研究所 |
| |
| 摘 要： | 将两株抗旋毛虫单克隆抗体（ＭｃＡｂ）杂交瘤细胞（Ｄ４，Ｅ２）所分泌的ＭｃＡｂ，经纯化后电泳分析，其重链分子质量为５５ｋｕ，轻链分子质量为２９ｋｕ，符合ＩｇＧ抗体的特点。两株ＭｃＡｂ与旋毛虫阳性猪血清所针对的旋毛虫抗原决定簇相同，都不与猪的其它寄生虫发生交叉反应，与抗原的相对亲和力Ｅ２＞Ｄ４，ＭｃＡｂ亲和层析纯化抗原的分子质量为４９ｋｕ，经免疫斑点染色证明为糖蛋白。
|
| 关 键 词： | 旋毛虫 杂交瘤细胞株 单克隆抗体 纯化抗原 |
Identification of Characteristics of Anti Trichinella spiralis Monoclonal Antibodies |
| |
| Wang Keling,Li Chunhua,Ma Zengquan,Yan Yuhe,Yuan Wenfu,Xu Weiguang.Identification of Characteristics of Anti Trichinella spiralis Monoclonal Antibodies[J].Veterinary Science in China,1999(8). |
| |
| Authors: | Wang Keling Li Chunhua Ma Zengquan Yan Yuhe Yuan Wenfu Xu Weiguang |
| |
| Abstract: | The monoclonal antibodies (McAbs) against
WTBX]Trichinella spiralis secreted by two strains of hybridomas (D 4,E 2) were purified and
analysed with electrophoresis, which revealed that the molecular masses of the heavy chain
and the light chain were 55 ku and 29 ku respectively which was in accord with the trait of IgG.
The two McAbs and the Trichinella spiralis postive pig's serum aimed at the same antigenic
determinant but no cross reaction with another parasites in swine occurred; the relative affinity
with antigen of E 2 was stronger than that of D 4. The molecular mass of McAb affinity
chromatography purified antigen was 49 ku. It was proved that the purified antigen was a
glucoprotein by means of an immunity dot staining test. |
| |
| Keywords: | Trichinella spiralis Hybridomas cell strain Monoclonal antibody Purified antigen |
| 本文献已被 CNKI 等数据库收录！ |
|
