| 禽脑脊髓炎病毒单克隆抗体的制备与初步应用 |
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| 引用本文： | 徐建生,唐波,成大荣,董国雄,吕玲,曹军.禽脑脊髓炎病毒单克隆抗体的制备与初步应用[J].中国兽医科学,2006,36(2):89-92. |
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| 作者姓名： | 徐建生 唐波 成大荣 董国雄 吕玲 曹军 |
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| 作者单位： | 扬州大学,兽医学院,江苏,扬州,225009 |
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| 摘 要： | 利用提纯的禽脑脊髓炎病毒(AEV)Van Roekel株作为免疫原,免疫6周龄BALB/c小鼠,采取其脾细胞与SP2/0骨髓瘤细胞融合,用间接ELISA法筛选,间接免疫荧光法(IFA)和免疫组织化学法鉴定,经3次亚克隆得到了稳定分泌抗AEV单克隆抗体的杂交瘤细胞株F11和G2,制备了腹水,并利用该单克隆抗体初步建立了Dot-ELISA、间接ELISA和IFA等特异性检测AEV抗原的方法。
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| 关 键 词： | 禽脑脊髓炎病毒 单克隆抗体 斑点-酶联免疫吸附法 |
| 文章编号： | 1673-4696(2006)02-0089-04 |
| 修稿时间： | 2005年8月29日 |
Preparation and preliminary application of monoclonal antibody to avian encephalomyelitis virus |
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| XU Jian-sheng,TANG Bo,CHENG Da-rong,DONG Guo-xiong,L Ling,CAO Jun.Preparation and preliminary application of monoclonal antibody to avian encephalomyelitis virus[J].Veterinary Science in China,2006,36(2):89-92. |
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| Authors: | XU Jian-sheng TANG Bo CHENG Da-rong DONG Guo-xiong L Ling CAO Jun |
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| Institution: | XU Jian-sheng,TANG Bo,CHENG Da-rong,DONG Guo-xiong,L(U) Ling,CAO Jun |
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| Abstract: | 6-week old BALB/c mice were immunized with purified avian encephalomyelitis viruses at two weeks interval.Mice were killed and their spleen cells fused into SP2/0 myeloma cells on day 3 after third immunization.Indirect-ELISA,indirect immunofluorescent assay and immunohistochemistry were employed to select and characterize the fused cells secreting antibody to AEV.As a result,two hybridoma cell lines steadily secreting antibody to AEV,named F_(11)and G_(2),were obtained after subcloning for three times.Ascites were prepared with the hybridoma and indirect fluorescent assay,indirect ELISA and Dot-ELISA were developed with the monoclonal antibody for the detection of AEV. |
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| Keywords: | avian encephalomyelitis virus monoclonal antibody Dot-ELISA |
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