| 利用DNA条形码技术鉴别植物 |
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| 引用本文： | 夏攀,曹禹,王创,许炎,周怀谷.利用DNA条形码技术鉴别植物[J].中国司法鉴定,2014(3):50-52. |
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| 作者姓名： | 夏攀 曹禹 王创 许炎 周怀谷 |
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| 作者单位： | 1. 上海市公安局物证鉴定中心上海市现场物证重点实验室公安部法医物证学现场应用技术重点实验室,上海,200083
2. 浙江大学植物生理学及生物化学国家重点实验室,浙江杭州,310058 |
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| 基金项目： | 公安部科技强警基础工作专项项目 |
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| 摘 要： | 目的研究利用DNA条形码技术鉴定上海地区常见植物叶片种类,在物种鉴定水平的基础上,进一步讨论利用分子标记技术对该类物证进行同一认定可行性。方法随机选取上海地区常见的9种植物,在其非新鲜的状态下利用高盐法提取植物组织的DNA,PCR扩增叶绿体rbcL和matK基因片段并且回收测序,通过数据库比对确定植物的物种。结果利用双基因鉴定分析系统,在随机收集的9份样品中,均达到物种水平的鉴定。结论利用DNA条形码技术,可以在物种水平准确地鉴定植物叶片类物证。
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| 关 键 词： | 法医遗传学 DNA条形码 植物 |
Application of the DNA Bar Code Technology in the Identification of Plant Species |
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| XIA Pan,CAO Yu,WANG Chuang,XU Yan,ZHOU Huai-gu.Application of the DNA Bar Code Technology in the Identification of Plant Species[J].Chinese Journal of Forensic Sciences,2014(3):50-52. |
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| Authors: | XIA Pan CAO Yu WANG Chuang XU Yan ZHOU Huai-gu |
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| Institution: | XIA Pan, CAO Yu, WANG Chuang, XU Yan, ZHOU Huai-gu (1. Institute of Forensic Science, Key Laboratory of Crime Scene Evidence, Shanghai Public Security Bureau; Key Laboratory of Site Application of Forensic Evidence and Technology, Ministry of Public Security, Shanghai 200083, China; 2. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China) |
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| Abstract: | Objective To identify the species of common plants in Shanghai by the DNA bar code technology, and to study the feasibility of identifying plant evidence with molecular markers. Method Nine different plant species were randomly selected in Shanghai. The plant DNA was extracted from these samples by an optimized high salt CTAB method. The rbeL and matK genes were amplified by PCR, The PCR products were sequenced and searched in the database. Results In the randomly collected nine different plant samples, the plant species were exactly identified by two gene systems. Conclusion The DNA bar code technology could be used to precisely identify plant species. |
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| Keywords: | forensic genetics DNA bar code plant |
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