应用DNA Typer15TM Direct试剂盒对河南地区汉族人群14个基因座遗传多态性的调查

目的调查河南地区汉族人群14个STR基因座的遗传多态性。同时简要介绍本实验室建库流程。方法应用DNA Typer15TM Direct试剂盒检测359名河南地区汉族无关个体14个STR基因座的等位基因频率,并应用统计软件计算群体遗传学参数。结果 14个STR基因座的基因型分布均符合Hardy-Weinberg平衡(P>0.05),14个基因座的杂合度(H)在0.694～0.922之间,匹配概率(Pm)在0.017～0.131之间,个人识别率(PD)在0.869～0.983之间,多态信息含量(PIC)在0.670～0.910之间,非父排除概率(PE)在0.418～0.841之间。结论 14个STR基因座在河南汉族人群中有较高的多态性,所得的群体遗传学数据可为法医学个人识别和亲子鉴定提供结果评估的依据。应用DNA Typer 15TM Direct试剂盒构建DNA数据库简单经济实用。

The application of DNA Typer15TM Direct kit in the DNA database construction

Objective To investigate the polymorphism of 14 STR loci in Henan Han population and briefly introduce the DNA database construction process.Methods DNA Typer15TM Direct kit was used to detect the allelic frequencies of 14 STR loci in 359 unrelated Henan Han individuals the population genetics and parameters were calculate by using statistic software.Results The results demonstrated that all loci were found to be no deviation from Hardy-Weinberg equilibrium(P>0.05).Heterozygosity(H)ranged from 0.694 to 0.922,matching probability(Pm)ranged from 0.017 to 0.131,power of discrimination(PD)ranged from 0.869 to 0.983,polymorphism information content(PIC)ranged from 0.670 to 0.910,power of exclusion(PE)ranged from 0.418 to 0.841.Conclusion The 14 STR loci are relatively abundant in polymorphic information for identification and paternity test in Henan Han population.Using DNA Typer15TM Direct kit to build a DNA database is very simple and economical.