| FTA卡直接扩增缓冲增强剂的研制 |
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| 引用本文： | 赵兴春,姜伯玮,叶健.FTA卡直接扩增缓冲增强剂的研制[J].刑事技术,2012(3):13-15. |
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| 作者姓名： | 赵兴春 姜伯玮 叶健 |
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| 作者单位： | 1. 公安部物证鉴定中心,北京,100038
2. 中科院上海应用物理研究所,201800 |
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| 摘 要： | 目的研制一种能够配合非直接扩增试剂应用的PCR缓冲增强剂,实现对FTA卡保存样本的直接扩增。方法基于常规STR复合扩增试剂盒的扩增体系及引物组,加入增强剂对FTA卡类样本进行直接扩增。结果获得样本STR分型结果清晰、完整、平衡性好,无明显抑制现象存在。结论所研制的FTA卡直扩增强剂能达到FTA卡保存样本的直接扩增检验要求,可应用于法医STR检验。
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| 关 键 词： | 法医遗传学 直接扩增 复合扩增 FTA卡 缓冲体系 增强剂 |
Development of PCR enhancer for direct amplification of samples on FTA cards |
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| ZHAO Xing-chun
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JIANG Bo-wei
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YE Jian.Development of PCR enhancer for direct amplification of samples on FTA cards[J].Forensic Science and Technology,2012(3):13-15. |
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| Authors: | ZHAO Xing-chun JIANG Bo-wei YE Jian |
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| Institution: | (Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China) |
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| Abstract: | Objective To validate the PCR enhancer for direct amplification of forensic DNA database samples stored on FTA cards. Methods Direct amplification on FTA punches were performed based on the use of commercialized STR amplification kit. Results High quality of STR profiles were obtained from described samples above. Conclusion The newly developed PCR enhancer can be applied to the area of forensics. |
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| Keywords: | forensic genetics direct amplification multiplex amplification FTA card buffer system PCR enhancer |
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