| 用引物Y_3、Y_4和PCR方法鉴定性别的法医学应用 |
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| 引用本文： | 顾丽华,刘珠耀,王令新,唐建平.用引物Y_3、Y_4和PCR方法鉴定性别的法医学应用[J].中国法医学杂志,1994(2). |
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| 作者姓名： | 顾丽华 刘珠耀 王令新 唐建平 |
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| 作者单位： | 上海市刑事科学技术研究所!200083 |
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| 摘 要： | 用Y3、Y4和Alu9．1、Alug．2两对引物和PCR方法检测陈旧血痕和毛根的性别获得成功。引物Y3、Y4扩增的靶序列位于Y染色体特异3．4Kb重复序列中，扩增产物为460bp；引物Alu9．1、Alu9.2用以扩增男女共有的Alu重复序列，扩增产物为130bP。室温保存13年之久的19例脐带血血痕（男性9冽，女性10例）和室温保存10～11个月的10例已知性别自然脱落毛根（男性6例，女性4例）的性别测定结果均正确；对一起凶杀案的血痕性别测定为定案提供了重要证据。本方法简化了样品的前处理过程。
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| 关 键 词： | 聚合酶链反应（PCR） 性别鉴定 血痕 毛根 |
THE SEX DETERMINATION OF THE BLOOD STAINS AND HAIR BY POLYMERASE CHAIN REACTION WITH PRIMERS Y3,Y4 |
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| Abstract: | We have succeeded in sex determination of old blood stains and hair roots by polymerase chain reaction (PCR) with primers Y3,Y4 and Alu9. 1,Alu9. 2. The target sequences of primers Y3, Y4 arelocated in 3. 4kb repeated sequences of Y-chromosome DNA and that of primers Alu9. 1, Alu9. 2 arecommon human gene. The length of amplified products of Y3,Y4 and Alu9. 1,Alu9. 2 are 460bp and130bp, respectively. The results of sex determination of fetal umbilical cord blood stains of thirteenyears old (1O samples of female, 9 samples of male) and natural - shed hair roots of 11 monthsagewere correct. This method was used in forensic medicine practice. The PCR method is simple and easyto perform. |
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| Keywords: | DNA polymerace chain reaction (PCR) Sex identification Blood stain Hair root |
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