| 血痕中PGM_1亚型的检出及酶谱的保存方法 |
| |
| 引用本文： | 王嵬贾,静涛.血痕中PGM_1亚型的检出及酶谱的保存方法[J].中国法医学杂志,1987(3). |
| |
| 作者姓名： | 王嵬贾 静涛 |
| |
| 作者单位： | 中国医科大学法医血清学教研室，中国医科大学法医血清学教研室 |
| |
| 摘 要： | 本文报告用等电聚焦方法,采用拉丁方设计,对血痕保存的温度、布质及含量,以及血痕中 PGM_1亚型检出时间进行了研究。保存在0℃(6个月)、4℃(2个月)、18℃(1个月)及30℃(3周)的6μL 血痕,PGM_1亚型均可检出。血痕的总量对 PGM_1亚型的检出时间也有一定的影响。不同布质对血痕中 PGM_1亚型的检出时间,无明显差异。另外,利用聚脂膜具有亲水膜面的特点,将 PGM_1原始酶谱贴附在聚酯膜上,可长期保存酶谱。
|
| 关 键 词： | 血痕 PGM_1亚型的检测 拉丁方设计 聚丙烯酰胺等电聚焦 |
PGM_1 SUBTYPING IN BLOODSTAINS AND A METHOD FOR PERMANENT STORAGE OF THE PGM1ZYMOGRAM |
| |
| Wang wei Jia Jingtao.PGM_1 SUBTYPING IN BLOODSTAINS AND A METHOD FOR PERMANENT STORAGE OF THE PGM1ZYMOGRAM[J].Chinese Journal of Forensic Medicine,1987(3). |
| |
| Authors: | Wang wei Jia Jingtao |
| |
| Institution: | Wang wei Jia Jingtao Department of Forensic Serology,China Medical University,Shenyang |
| |
| Abstract: | The effects of the temperature,materials and quantities of blood on results of PGM_1 phenotyping in
bloodstains in a latin square were studied using polyacrylamide gel isoelectric focusing.The detectable
time of the bloodstains kept at 0℃,4℃,18℃ and 30℃ were 6 months,2 months,1 months and
3 weeks respectively.The quantity of blood staining also had an effect on the detectable time of PGM_1
subtyping in bloodstains.However,there was not any significant difference of detectable time of PGM_1
subtyping in bloodstains on different materials was observed.In addition,a method for permanent storage
of zymogram was developed using polyester sheet. |
| |
| Keywords: | PGM_1 subtyping in blood stains Latin square design Polyacrylamide gel isoelelectric focusing |
| 本文献已被 CNKI 等数据库收录！ |
|
