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| 用于DNA分离的毛细管电泳无胶筛分介质研究进展 | |
| 引用本文： | 王燕,赵兴春,姜伯玮,孙敬,叶健.用于DNA分离的毛细管电泳无胶筛分介质研究进展[J].中国法医学杂志,2012,27(1):41-44. |
| 作者姓名： | 王燕 赵兴春 姜伯玮 孙敬 叶健 |
| 作者单位： | 1. 中国人民公安大学,北京,100038 2. 公安部物证鉴定中心,北京,100038 3. 中国科学院上海应用物理研究所,上海,201806 |
| 基金项目： | 国家科技支撑计划资助项目(2008BAK45B04) |
| 摘 要： | 毛细管电泳是一种在生命科学应用最为广泛的生物分子分离技术,目前已广泛应用于DNA测序,SNP检测,DNA片段分析等。筛分介质是毛细管电泳性能的关键因素,筛分介质的种类、结构、分子量及所形成的溶液决定着生物分子的迁移特性、分辨率、读出长度、重现性等参数,对分离结果有重大影响。本文对近年来用于DNA分离的无胶筛分介质的研究和应用情况进行综述,希望能对相关研究和鉴定提供参考和借鉴。 |
| 关 键 词： | 法医遗传学 毛细管电泳 筛分介质 聚合物 |
Research progress on sieving media of capillary electrophoresis in DNA separation |
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| Wang Yan , Zhao Xingchun , Jiang Bowei , Sun Jing , Ye Jian.Research progress on sieving media of capillary electrophoresis in DNA separation[J].Chinese Journal of Forensic Medicine,2012,27(1):41-44. | |
| Authors: | Wang Yan Zhao Xingchun Jiang Bowei Sun Jing Ye Jian |
| Institution: | 1.Chinese Peple’s Public Security University,Beijing 100038,China;2.Institute of Forensic Science Ministry of Public Security,Beijing 100038,China;3.Shanghai Institute of Applied Physics,Chinese Academy of Science,Shanghai 201806,China) |
| Abstract: | Capillary electrophoresis technology,as one kind of biomolecule separation methods in life science,has been widely used in DNA sequencing,SNP and DNA fragment analysis,etc.Sieving media is a key factor in the performance capillary electrophoresis,and its category,conformation and related property will determine the migration characteristics of biological molecules,resolution,length of read and reproducibility.Therefore,sieving media has a significant influence on the separation results.A review on polymers used as DNA separation media in capillary electrophoresis is presented. |
| Keywords: | forensic genetics capillary electrophoresis sieving matrix polymers |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |