心肌早期缺血再灌流损伤HSP70表达研究

目的　探讨早期缺血再灌流损伤心肌组织热休克蛋白 70 (HSP70 )的变化 ,为心源性猝死的法医病理学鉴定寻找新的依据。　方法　建立家兔早期心肌缺血再灌流动物模型 ,用免疫组化SABC法观察HSP70的表达。　结果　心肌缺血 15min再灌流 30min后 ,再灌流区心内膜下可见HSP70阳性表达 ;缺血30min再灌流 30min后 ,HSP70阳性反应细胞数目较多 ,散布于心肌全层 ;缺血 6 0min再灌流 30min后 ,再灌流区阳性表达明显减少 ,而其边缘见阳性表达略有增强。各实验组正常区和对照组心肌组织均未见阳性反应。　结论　心肌组织HSP70的阳性表达是一项显示心肌早期缺血再灌流损伤的灵敏指标 ,HSP70的免疫组化检测对缺血再灌流损伤所致心源性猝死的死后诊断具有一定的应用价值。

Expression of HSP70 in early myocardial ischemia and reperfusion injury.

Objective To study the changes of the expression of HSP70 in myocardial tissue after early myocardial ischemia and reperfusion injury (MI/R), and search a new evidence for forensic pathological diagnosis in case of sudden cardiac death. Methods The rabbit model of MI/R was established, the expression of HSP70 in myocardial tissue was observe used immunohistochemical SABC method. Results The expression of HSP70 was detected in the subendothelial area of MI/R after 15 min ischemia followed by 30 min reperfusion. The positive expression of HSP70 was diffused in all myocardial tissue of MI/R after 30 min ischemia followed by 30 min reperfusion. However, the positive expression was markedly decreased in the area of MI/R but slightly increased in its margin area after 60 min ischemia followed by 30 min reperfusion. The negative staining in myocardium was found in the control group and nonischemic experimental groups. Conclusion The positive expression of HSP70 in myocardial tissue is a sensitive marker of MI/R. The detection expression of HSP70 can helpful to the postmortem diagnosis of sudden cardiac death caused by MI/R.