微量生物物证提取套装在现场微量血痕DNA检验中的应用

目的探讨微量生物物证提取套装应用于提取现场微量血痕DNA的检验效果。方法将静脉血制成地面血痕。分别应用微量生物物证提取套装法和普通法提取血痕。分别于恒温摇床放置2、24、48、72、96h(每组50份)进行血痕DNA检验,对比各组检验结果。结果恒温摇床上分别放置24、48、72、96h后,微量生物物证提取套装法的DNA检出率(分别为100.00%、100.00%、100.00%、96.00%)均高于普通法(分别为62.00%、26.00%、10.00%、0),差异均具有统计学意义(P<0.05)。恒温摇床上放置2h,两种方法的DNA检出率差异无统计学意义(P>0.05)。结论微量生物物证提取套装可有效提升放置时间较久的现场微量血痕的检出率和检出时限。

Application of Trace Biological Evidence Collection Kit in DNA Examination of Trace Bloodstain Samples from the Scene

Objective To evaluate the effects of DNA examination of trace bloodstain samples from the scene collected with Trace Biological Evidence Collection kit.Methods Venous blood was made into bloodstains on the ground.The trace bloodstain samples were collected with Trace Biological Evidence Collection kit and common methods,respectively.DNA examination of trace bloodstain samples(50 from each group)was conducted on the constant temperature shaker for 2,24,48,72,and 96 h,respectively,and the examination results of every group were compared.Results When the trace bloodstain samples were placed on the constant temperature shaker for 24,48,72,and 96 h,the DNA detection rates in the group which used Trace Biological Evidence Collection kit(100.00%,100.00%,100.00%,96.00%)were significantly higher than those in the group using common methods(62.00%,26.00%,10.00%,0),the differences had statistical significance(P<0.05).When the trace bloodstain samples were placed on the constant temperature shaker for 2 h,the differences of DNA detection rates between the two groups had no statistical significance(P>0.05).Conclusion The Trace Biological Evidence Collection kit can effectively improve DNA detection rate and extend detection time limit for trace bloodstain samples from the scene that have been stored for a relatively long time.