病毒性心肌炎和扩张型心肌病心肌Fas蛋白表达

目的探讨病毒性心肌炎（viral myoearditis，VMC）和扩张型心肌病（dilated cardiomyopathy，DCM）的发病机制及相互关系。方法应用改良的免疫组织化学技术观察VMC、DCM和对照组（各20例）心肌Fas蛋白的表达，并对其阳性反应结果进行Ridit检验。结果与对照组相比，VMC和DCM组Fas蛋白阳性表达明显升高，经Ridit检验，3组阳性结果差异有统计学意义（P〈O．005）；进一步采用两两比较Ridit检验，对照组和其他两组之间差异有统计学意义（P〈O．05），而VMC和DCM组间差异无统计学意义（P〉0．05）。结论VMC和DCM心肌组织中Fas蛋白表达明显高于对照组，提示VMC和DCM的发病都有细胞凋亡的参与：凋亡可能是VMC引起心肌损伤及演变为心肌病的一个发病机制。

Expression of Fas Protein in Myocardiac Tissue of Viral Myocarditis and Dilated Cardiomyopathy

Objective To study the pathogenesis of viral myocarditis （VMC） and dilated cardiomyopathy （DCM） and their relationship. Methods Sixty samples including 20 VMC, 20 DCM and 20 controls were collected. The expression of Fas protein in myocardium of each group was deteeted by modified immuno-histochemistry with unequivocal brown staining in the myocardial membrane scored as positive, and the results of positive reaction were analyzed By Ridit test. Results Fas protein expression increased obviously in VMC and DCM groups as compared with that of the control group. The difference of positive results Between each group analyzed by Ridit test was statistically significant （P〈0.005）. Statistically significant differences were found between VMC and control groups as well as between DCM and control groups （P〈0.05）, but not between VMC and DCM groups（P〉0.05） by multiple comparison Ridit test. Conclusion The expression of Fas protein is significantly higher in the VMC and DCM groups than in that of the control group. These results suggest that both the VMC and DCM may share a similar pathogenesis, which most likely involves cell apoptosis.