| UPLC-MS/MS分析尿液中阿片类化合物 |
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| 引用本文： | 展长淑,沈保华,卓先义.UPLC-MS/MS分析尿液中阿片类化合物[J].法医学杂志,2010,26(3):188-191. |
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| 作者姓名： | 展长淑 沈保华 卓先义 |
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| 作者单位： | 1. 苏州大学,医学部法医学系,江苏,苏州,215123;司法部司法鉴定科学技术研究所,上海市法医学重点实验室,上海,200063
2. 司法部司法鉴定科学技术研究所,上海市法医学重点实验室,上海,200063 |
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| 基金项目： | 国家质检公益性行业科研专项，科研院所社会公益研究专项 |
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| 摘 要： | 目的 建立尿液中同时分析可待因(codeine,COD)、6-单乙酰吗啡(6-monoacetylmorphine,6-MAM)、吗啡(morphine,MOR)、吗啡-3-葡萄糖醛酸苷(morphine-3-glucuronide,M3G)和吗啡-6-葡萄糖醛酸苷(morphine-6-glucuronide,M6G)的超高效液相色谱-串联质谱(UPLC-MS/MS)方法.方法 以吗啡-d3(MOR-d3)和吗啡-3-葡萄糖醛酸苷-d3(M3G-d3)为内标,尿液用乙腈沉淀蛋白后,过SiroccoTM蛋白沉淀板,UPLC-MS/MS法分离检测.结果 尿液中COD和MAM检出限为0.2 ng/Ml,定量限为0.5 ng/Ml;MOR、M3G和M6G检出限为0.5 ng/Ml,定量限为1 ng/Ml;线性相关系数r≥0.999 7;日内精密度和日间精密度均在10%以内;回收率70.0%～98.3%,基质效应50.5%～99.0%.结论 所建方法简便、快速、准确,可以满足法庭毒物分析的需要.
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| 关 键 词： | 法医毒理学 可待因 海洛因 尿 |
Simultaneous Determination of Opioid Compounds in Human Urine by UPLC-MS/MS |
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| ZHAN Chang-shu,SHEN Bao-hua,ZHUO Xian-yi.Simultaneous Determination of Opioid Compounds in Human Urine by UPLC-MS/MS[J].Journal of Forensic Medicine,2010,26(3):188-191. |
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| Authors: | ZHAN Chang-shu SHEN Bao-hua ZHUO Xian-yi |
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| Institution: | ZHAN Chang-shu1,2,SHEN Bao-hua2,ZHUO Xian-yi2(1.Department of Forensic Medicine,Medical College of Soochow University,Suzhou 215123,China,2.Shanghai Key Laboratory of Forensic Medicine,Institute of Forensic Science,Ministry of Justice,P.R.China,Shanghai 200063,China) |
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| Abstract: | Objective To propose a method for simultaneous determination of codeine(COD),6-monoacetyl-morphine(6-MAM),morphine(MOR),morphine-3-glucuronide(M3G) and morphine-6-glucuronide(M6G) in human urine by ultra performance liquid chromatography with tandem mass spectrometry(UPLC-MS/MS).Methods After precipitation of protein by acetonitrile,the urine samples,with added the morphine-d3(MOR-d3) and morphine-3-Glucuronide-d3(M3G-d3) as internal standards,were pre-treated by SiroccoTM protein precipitation plate,and th... |
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| Keywords: | UPLC-MS/MS |
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