GC—MS同时测定血液中苯海索、氯丙嗪和氯氮平

目的建立GC—MS同时测定血液中苯海索、氯丙嗪和氯氮平的方法。方法血液中加入内标SKFszs。．在pH〉10条件下用V（苯）：v（乙酸乙酯）=1：l提取,用GC—MS全扫描法进行定性检测：以地西泮一d;为内标,样品在pH〉10条件下用V（苯）：v（乙酸乙酯）=l：1提取,用GC—MS选择离子监测方法进行定量检测．结果苯海索、氯丙嗪和氯氮平在20～10000ng／mL范围内呈线性关系,最小检测限分别为0．3、0．3和0．7n幽L（信噪比≥3）,方法回收率为79．9％～85．5％,日内、日间精密度均小于5．1％。结论本方法可同时分析血液中苯海索、氯丙嗪和氯氮平,方法灵敏度高、快速、操作简便,适用于苯海索、氯丙嗪和氯氮平的血药浓度监测和急性中喜案件竹枪测

Simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in blood by GC-MS

Objective To develop a method to measure trihexyphenidyl, chlorpromazine and clozapine in human blood with GC-MS. Methods The specimens were alkalized （pH〉10） and extracted with V（ben- zene）：V （ethyl acetate）= 1：1, and qualitatively analyzed using GC-MS-Full Scan with internal standard SKFs~A. The specimens were alkalized（pH〉10） and extracted with V（benzene）：V（ethyl acetate ） =l ： l, and quantita- tively analyzed using GC-MS-SIM with internal standard diazepam-ds. Results The lowest detection limits of trihexyphenidyl, chlorpromazine and clozapine were 0.3, 0.3 and 0.7ng/mL （S/N≥3） respectively. The calibration curve in 20-10 000 ng/mL showed a good linear distribution. The recovery rate was 79.9% to 85.5%. The RSDs of intraday and interday were less than 5.1%. Conclusion The established method was simple, sensitive and accurate for simultaneous determination of trihexyphenidyl, ehlorpromazine and elozap- ine in human blood, and can be applied in forensic toxicological cases.