犬11个STR基因座的遗传多态性

目的调查犬11个STR基因座的群体遗传多态性。方法应用自主构建的犬11个STR基因座（PEZ1、PEZ2、PEZ3、PEZ5、PEZ6、PEZ8、PEZ12、FH2010、FH2054、FH2132和FH2611）荧光复合扩增体系，扩增105只犬的样本，统计各基因座扩增结果，并分析其群体遗传参数。结果11个STR基因座的累积非父排除率和累积个体识别率分别为0．9330621和0．9999999．平均杂和度和平均多态信息含量分别为0．502和0．640。结论该11个犬STR基因座的遗传多态性较好，可以有效用于犬的个体识别和亲权鉴定。

Genetic Polymorphism of Eleven Canine STR Loci

Objective To investigate the polymorphism of 11 canine STR loci. Methods A fluorescent multiplex system with 11 STR loci（PEZI, PEZ2, PEZ3, PEZ5, PEZ6, PEZ8, PEZ12, FH2010, FH2054, FH2132 and FH2611） was constructed independently and performed to amplify 105 samples from dogs. The character of these loci was analyzed with the PCR data. Results The distributions of genotypes and allele frequencies of 11 STR loci were obtained. The total power of discrimination for the 11 loci in canine population was 0.999 999 9 and the cumulative probability of exclusion was 0.933 062 1. The observed heterozygosity and polymorphism information content（PIC） were 0.502 and 0.640, respectively. Conclusion Each of the eleven canine STR loci has a high genetic polymorphism and can be applied for the parentage testing and individual identification. The fluorescent multiplex system is a reliable method in forensic application.