| 气相色谱-串联质谱法测定血液中乙基葡萄糖醛酸苷 |
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| 引用本文： | 沈保华,严慧,赵晖,卓先义.气相色谱-串联质谱法测定血液中乙基葡萄糖醛酸苷[J].法医学杂志,2009,25(6):431-433. |
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| 作者姓名： | 沈保华 严慧 赵晖 卓先义 |
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| 作者单位： | 1. 司法部司法鉴定科学技术研究所,上海市法医学重点实验室,上海,200063
2. 司法部司法鉴定科学技术研究所,上海市法医学重点实验室,上海,200063;复旦大学,上海医学院,上海,200032 |
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| 基金项目： | 国家科技部公益基金资助项目 |
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| 摘 要： | 目的建立血液中乙基葡萄糖醛酸苷（ethyl glucuronide,EtG）的气相色谱-串联质谱（GC-MS/MS）测定方法。方法血液用乙腈沉淀蛋白,离心后,将上清液转移吹干,残留物经N,O-双（三甲基硅烷基）三氟乙酰胺衍生化,用GC-MS/MS进行检测。结果血液中EtG的检出限为0.05μg/mL,线性范围为0.1～10μg/mL（r=0.9999）。对送检案例血液检材进行EtG检测,效果良好。结论本方法适用于血液中EtG的检测。
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| 关 键 词： | 法医毒理学 血液 乙基葡萄糖醛酸苷 气相色谱-串联质谱法 |
Determination of Ethyl Glucuronide in Blood by GC-MS/MS |
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| SHEN Bao-hua,YAN Hui,ZHAO Hui,ZHUO Xian-yi.Determination of Ethyl Glucuronide in Blood by GC-MS/MS[J].Journal of Forensic Medicine,2009,25(6):431-433. |
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| Authors: | SHEN Bao-hua YAN Hui ZHAO Hui ZHUO Xian-yi |
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| Institution: | SHEN Bao-hua1,YAN Hui1,ZHAO Hui1,2,ZHUO Xian-yi1 (1. Shanghai Key Laboratory of Forensic Medicine,Institute of Forensic Science,Ministry of Justice,P.R.China,Shanghai 200063,China,2. Shanghai Medical College,Fudan University,Shanghai 200032,China) |
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| Abstract: | Objective To develop a method for determining ethyl glucuronide（EtG） in human blood with gas chromatograph-tandem mass spectrometry （GC-MS/MS）. Methods Human blood protein was precipitated with acetonitrile. The supernatant was transferred and air flow dried after centrifugated. The residue was derived with N, O -bis （trimethylsilyl）trifluoroacetamide （BSTFA）, and analyzed with GC -MS/MS. Results The detection limit of EtG in blood was 0.05 μg/mL. Calibration curve covered a span from 0.1-10 μg/mL with a good linear relationship （r=0.999 9）. The method showed a excellent performance when was used to authentic blood sample analysis for EtG. Conclusion The method is suitable for blood EtG analysis. |
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| Keywords: | forensic toxicology blood ethyl glucuronide GC-MS/MS |
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